ID   LUXA1_PHOLU             Reviewed;         360 AA.
AC   P19839;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=Alkanal monooxygenase alpha chain;
DE            EC=1.14.14.3 {ECO:0000269|PubMed:2204626};
DE   AltName: Full=Bacterial luciferase alpha chain;
GN   Name=luxA;
OS   Photorhabdus luminescens (Xenorhabdus luminescens).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Morganellaceae; Photorhabdus.
OX   NCBI_TaxID=29488;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND CATALYTIC ACTIVITY.
RC   STRAIN=ATCC 29999 / DSM 3368 / BCRC 14801 / CIP 106429 / NCIMB 12670 / Hb;
RX   PubMed=2204626; DOI=10.1016/s0021-9258(17)46262-4;
RA   Szittner R., Meighen E.;
RT   "Nucleotide sequence, expression, and properties of luciferase coded by lux
RT   genes from a terrestrial bacterium.";
RL   J. Biol. Chem. 265:16581-16587(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 29999 / DSM 3368 / BCRC 14801 / CIP 106429 / NCIMB 12670 / Hb;
RX   PubMed=1644764; DOI=10.1128/jb.174.16.5371-5381.1992;
RA   Meighen E.A., Szittner R.B.;
RT   "Multiple repetitive elements and organization of the lux operons of
RT   luminescent terrestrial bacteria.";
RL   J. Bacteriol. 174:5371-5381(1992).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Hm;
RX   PubMed=2383248; DOI=10.1016/0006-291x(90)92106-a;
RA   Johnston T.C., Rucker E.B., Cochrum L., Hruska K.S., Vandegrift V.;
RT   "The nucleotide sequence of the luxA and luxB genes of Xenorhabdus
RT   luminescens HM and a comparison of the amino acid sequences of luciferases
RT   from four species of bioluminescent bacteria.";
RL   Biochem. Biophys. Res. Commun. 170:407-415(1990).
CC   -!- FUNCTION: Light-emitting reaction in luminous bacteria.
CC       {ECO:0000269|PubMed:2204626}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a long-chain fatty aldehyde + FMNH2 + O2 = a long-chain fatty
CC         acid + FMN + 2 H(+) + H2O + hnu; Xref=Rhea:RHEA:17181,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:17176, ChEBI:CHEBI:30212, ChEBI:CHEBI:57560,
CC         ChEBI:CHEBI:57618, ChEBI:CHEBI:58210; EC=1.14.14.3;
CC         Evidence={ECO:0000269|PubMed:2204626};
CC   -!- SUBUNIT: Heterodimer of an alpha and a beta chain.
CC       {ECO:0000250|UniProtKB:P07740}.
CC   -!- MISCELLANEOUS: The synthesis of this protein is regulated by a complex
CC       control mechanism that has been termed autoinduction.
CC   -!- SIMILARITY: Belongs to the bacterial luciferase oxidoreductase family.
CC       {ECO:0000305}.
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DR   EMBL; M57416; AAA27623.1; -; Genomic_DNA.
DR   EMBL; M90093; AAA27619.1; -; Genomic_DNA.
DR   EMBL; M55977; AAA27626.1; -; Genomic_DNA.
DR   PIR; B35411; B35411.
DR   RefSeq; WP_033875256.1; NZ_FMWJ01000001.1.
DR   AlphaFoldDB; P19839; -.
DR   SMR; P19839; -.
DR   STRING; 29488.KS18_21620; -.
DR   PRIDE; P19839; -.
DR   GeneID; 45654919; -.
DR   KEGG; ag:AAA27623; -.
DR   BRENDA; 1.14.14.3; 4782.
DR   GO; GO:0047646; F:alkanal monooxygenase (FMN-linked) activity; IEA:UniProtKB-EC.
DR   GO; GO:0008218; P:bioluminescence; IEA:UniProtKB-KW.
DR   CDD; cd01096; Alkanal_monooxygenase; 1.
DR   Gene3D; 3.20.20.30; -; 1.
DR   InterPro; IPR033924; Alkanal_monooxygenase.
DR   InterPro; IPR018235; Bacterial_luciferase_CS.
DR   InterPro; IPR011251; Luciferase-like_dom.
DR   InterPro; IPR036661; Luciferase-like_sf.
DR   InterPro; IPR002103; Luciferase_bac/NFP.
DR   Pfam; PF00296; Bac_luciferase; 1.
DR   PRINTS; PR00089; LUCIFERASE.
DR   SUPFAM; SSF51679; SSF51679; 1.
DR   PROSITE; PS00494; BACTERIAL_LUCIFERASE; 1.
PE   1: Evidence at protein level;
KW   Flavoprotein; FMN; Luminescence; Monooxygenase; Oxidoreductase;
KW   Photoprotein.
FT   CHAIN           1..360
FT                   /note="Alkanal monooxygenase alpha chain"
FT                   /id="PRO_0000220167"
SQ   SEQUENCE   360 AA;  41001 MW;  9410729A95DFB93E CRC64;
     MKFGNFLLTY QPPQFSQTEV MKRLVKLGRI SEECGFDTVW LLEHHFTEFG LLGNPYVAAA
     YLLGATKKLN VGTAAIVLPT AHPVRQLEDV NLLDQMSKGR FRFGICRGLY NKDFRVFGTD
     MNNSRALAEC WYGLIKNGMT EGYMEADNEH IKFHKVKVNP AAYSRGGAPV YVVAESASTT
     EWAAQFGLPM ILSWIINTNE KKAQLELYNE VAQEYGHDIH NIDHCLSYIT SVDHDSIKAK
     EICRKFLGHW YDSYVNATTI FDDSDQTRGY DFNKGQWRDF VLKGHKDTNR RIDYSYEINP
     VGTPQECIDI IQKDIDATGI SNICCGFEAN GTVDEIIASM KLFQSDVMPF LKEKQRSLLY