ID   LUXA_ALIFS              Reviewed;         354 AA.
AC   P19907;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Alkanal monooxygenase alpha chain;
DE            EC=1.14.14.3 {ECO:0000250|UniProtKB:P24113};
DE   AltName: Full=Bacterial luciferase alpha chain;
GN   Name=luxA;
OS   Aliivibrio fischeri (Vibrio fischeri).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Aliivibrio.
OX   NCBI_TaxID=668;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=MJ-1;
RX   PubMed=3340562; DOI=10.1093/nar/16.2.777;
RA   Foran D.R., Brown W.M.;
RT   "Nucleotide sequence of the LuxA and LuxB genes of the bioluminescent
RT   marine bacterium Vibrio fischeri.";
RL   Nucleic Acids Res. 16:777-777(1988).
CC   -!- FUNCTION: Light-emitting reaction in luminous bacteria.
CC       {ECO:0000250|UniProtKB:P24113}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a long-chain fatty aldehyde + FMNH2 + O2 = a long-chain fatty
CC         acid + FMN + 2 H(+) + H2O + hnu; Xref=Rhea:RHEA:17181,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:17176, ChEBI:CHEBI:30212, ChEBI:CHEBI:57560,
CC         ChEBI:CHEBI:57618, ChEBI:CHEBI:58210; EC=1.14.14.3;
CC         Evidence={ECO:0000250|UniProtKB:P24113};
CC   -!- SUBUNIT: Heterodimer of an alpha and a beta chain.
CC       {ECO:0000250|UniProtKB:P07740}.
CC   -!- SIMILARITY: Belongs to the bacterial luciferase oxidoreductase family.
CC       {ECO:0000305}.
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DR   EMBL; X06758; CAA29931.1; -; Genomic_DNA.
DR   EMBL; U55385; AAB00442.1; -; Genomic_DNA.
DR   EMBL; U55819; AAB00449.1; -; Genomic_DNA.
DR   PIR; S00574; S00574.
DR   AlphaFoldDB; P19907; -.
DR   SMR; P19907; -.
DR   BioCyc; MetaCyc:LUXAVIBFI-MON; -.
DR   BRENDA; 1.14.14.3; 71.
DR   GO; GO:0047646; F:alkanal monooxygenase (FMN-linked) activity; IEA:UniProtKB-EC.
DR   GO; GO:0008218; P:bioluminescence; IMP:CACAO.
DR   GO; GO:0052043; P:modification by symbiont of host cellular component; IMP:CACAO.
DR   CDD; cd01096; Alkanal_monooxygenase; 1.
DR   Gene3D; 3.20.20.30; -; 1.
DR   InterPro; IPR033924; Alkanal_monooxygenase.
DR   InterPro; IPR018235; Bacterial_luciferase_CS.
DR   InterPro; IPR011251; Luciferase-like_dom.
DR   InterPro; IPR036661; Luciferase-like_sf.
DR   InterPro; IPR002103; Luciferase_bac/NFP.
DR   Pfam; PF00296; Bac_luciferase; 1.
DR   PRINTS; PR00089; LUCIFERASE.
DR   SUPFAM; SSF51679; SSF51679; 1.
DR   PROSITE; PS00494; BACTERIAL_LUCIFERASE; 1.
PE   3: Inferred from homology;
KW   Flavoprotein; FMN; Luminescence; Monooxygenase; Oxidoreductase;
KW   Photoprotein.
FT   CHAIN           1..354
FT                   /note="Alkanal monooxygenase alpha chain"
FT                   /id="PRO_0000220171"
SQ   SEQUENCE   354 AA;  40343 MW;  E8C403581DD5A318 CRC64;
     MKFGNICFSY QPPGETHKLS NGSLCSAWYR LRRVGFDTYW TLEHHFTEFG LTGNLFVAAA
     NLLGRTKTLN VGTMGVVIPT AHPVRQLEDV LLLDQMSKGR FNFGTVRGLY HKDFRVFGVD
     MEESRAITQN FYQMIMESLQ TGTISSDSDY IQFPKVDVYP KVYSKNVPTC MTAESASTTE
     WLAIQGLPMV LSWIIGTNEK KAQMELYNEI ATEYGHDISK IDHCMTYICS VDDDAQKAQD
     VCREFLKNWY DSYVNATNIF NDSNQTRGYD YHKGQWRDFV LQGHTNTNRR VDYSNGINPV
     GTPEQCIEII QRDIDATGIT NITCGFEANG TEDEIIASMR RFMTQVAPFL KEPK