ID   LUXA_KRYAS              Reviewed;         357 AA.
AC   P18299;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1990, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Alkanal monooxygenase alpha chain;
DE            EC=1.14.14.3 {ECO:0000250|UniProtKB:P19839};
DE   AltName: Full=Bacterial luciferase alpha chain;
GN   Name=luxA;
OS   Kryptophanaron alfredi symbiont.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae.
OX   NCBI_TaxID=28177;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2256783; DOI=10.1007/bf00245234;
RA   Haygood M.G.;
RT   "Relationship of the luminous bacterial symbiont of the Caribbean
RT   flashlight fish, Kryptophanaron alfredi (family Anomalopidae) to other
RT   luminous bacteria based on bacterial luciferase (luxA) genes.";
RL   Arch. Microbiol. 154:496-503(1990).
CC   -!- FUNCTION: Light-emitting reaction in luminous bacteria.
CC       {ECO:0000250|UniProtKB:P19839}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a long-chain fatty aldehyde + FMNH2 + O2 = a long-chain fatty
CC         acid + FMN + 2 H(+) + H2O + hnu; Xref=Rhea:RHEA:17181,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:17176, ChEBI:CHEBI:30212, ChEBI:CHEBI:57560,
CC         ChEBI:CHEBI:57618, ChEBI:CHEBI:58210; EC=1.14.14.3;
CC         Evidence={ECO:0000250|UniProtKB:P19839};
CC   -!- SUBUNIT: Heterodimer of an alpha and a beta chain.
CC       {ECO:0000250|UniProtKB:P07740}.
CC   -!- SIMILARITY: Belongs to the bacterial luciferase oxidoreductase family.
CC       {ECO:0000305}.
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DR   EMBL; M36597; AAA91213.1; -; Genomic_DNA.
DR   AlphaFoldDB; P18299; -.
DR   SMR; P18299; -.
DR   GO; GO:0047646; F:alkanal monooxygenase (FMN-linked) activity; IEA:UniProtKB-EC.
DR   GO; GO:0008218; P:bioluminescence; IEA:UniProtKB-KW.
DR   CDD; cd01096; Alkanal_monooxygenase; 1.
DR   Gene3D; 3.20.20.30; -; 1.
DR   InterPro; IPR033924; Alkanal_monooxygenase.
DR   InterPro; IPR018235; Bacterial_luciferase_CS.
DR   InterPro; IPR011251; Luciferase-like_dom.
DR   InterPro; IPR036661; Luciferase-like_sf.
DR   InterPro; IPR002103; Luciferase_bac/NFP.
DR   Pfam; PF00296; Bac_luciferase; 1.
DR   PRINTS; PR00089; LUCIFERASE.
DR   SUPFAM; SSF51679; SSF51679; 1.
DR   PROSITE; PS00494; BACTERIAL_LUCIFERASE; 1.
PE   3: Inferred from homology;
KW   Flavoprotein; FMN; Luminescence; Monooxygenase; Oxidoreductase;
KW   Photoprotein.
FT   CHAIN           1..357
FT                   /note="Alkanal monooxygenase alpha chain"
FT                   /id="PRO_0000220163"
SQ   SEQUENCE   357 AA;  40386 MW;  36842D3F6E3DECDF CRC64;
     MKFGNFLLTY QPPQLDQKEV IKRLVNLGQA SESCGFDTAW LLEHHFTEFG LLGNPYVAAA
     NLLGATKKLH VGTAAVVLPT AHPVRQLEDV NLLDQMSKGR FKFGICRGLY DKDFRVFGTD
     MSNSRELMNS WYDIMTKGMI EGHVSSDNEH IKFPKVKVSP NSYTQRGAPV YVVAESASTT
     EWAAERGLPI ILSWIINNNE KKSQLDLYNE IALEHGHDVS NIDHCMSYIT SVDHNSNKAK
     DICRDFLAHW YDSYLNATSI FDDSNQTKGY DFNKGQWRNF VLKGHKDTNR RIDYSYEINP
     VGTPQECIEI IQSDIDATGI HNICCGFEAN GSETEIIASM KLFQSDVMPY LKEKSNC