ID   LUXA_VIBCL              Reviewed;         103 AA.
AC   P24114;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-1992, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Alkanal monooxygenase alpha chain;
DE            EC=1.14.14.3 {ECO:0000250|UniProtKB:P19839};
DE   AltName: Full=Bacterial luciferase alpha chain;
DE   Flags: Fragment;
GN   Name=luxA;
OS   Vibrio cholerae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=666;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 14547 / LMG 4406 / NCIMB 41 / CIP 69.41, UM4082, and UM4102;
RX   PubMed=1854193; DOI=10.1128/aem.57.5.1286-1293.1991;
RA   Palmer L.M., Colwell R.R.;
RT   "Detection of luciferase gene sequence in nonluminescent Vibrio cholerae by
RT   colony hybridization and polymerase chain reaction.";
RL   Appl. Environ. Microbiol. 57:1286-1293(1991).
CC   -!- FUNCTION: Light-emitting reaction in luminous bacteria.
CC       {ECO:0000250|UniProtKB:P19839}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a long-chain fatty aldehyde + FMNH2 + O2 = a long-chain fatty
CC         acid + FMN + 2 H(+) + H2O + hnu; Xref=Rhea:RHEA:17181,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:17176, ChEBI:CHEBI:30212, ChEBI:CHEBI:57560,
CC         ChEBI:CHEBI:57618, ChEBI:CHEBI:58210; EC=1.14.14.3;
CC         Evidence={ECO:0000250|UniProtKB:P19839};
CC   -!- SUBUNIT: Heterodimer of an alpha and a beta chain.
CC       {ECO:0000250|UniProtKB:P07740}.
CC   -!- MISCELLANEOUS: The synthesis of this protein is regulated by a complex
CC       control mechanism that has been termed autoinduction. In fully induced
CC       cells luciferase comprises up to 5% of the soluble protein.
CC   -!- MISCELLANEOUS: Strain ATCC 14547 is luminescent.
CC   -!- MISCELLANEOUS: Strain UM4102 is dimly luminescent.
CC   -!- MISCELLANEOUS: Strain UM4082 is non-luminescent.
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DR   EMBL; M60437; AAA27532.1; -; Genomic_DNA.
DR   EMBL; M60438; AAA27533.1; -; Genomic_DNA.
DR   EMBL; M60439; AAA27534.1; -; Genomic_DNA.
DR   PIR; B49775; B49775.
DR   PIR; C49775; C49775.
DR   AlphaFoldDB; P24114; -.
DR   SMR; P24114; -.
DR   GO; GO:0047646; F:alkanal monooxygenase (FMN-linked) activity; IEA:UniProtKB-EC.
DR   GO; GO:0008218; P:bioluminescence; IEA:UniProtKB-KW.
DR   Gene3D; 3.20.20.30; -; 1.
DR   InterPro; IPR036661; Luciferase-like_sf.
DR   InterPro; IPR002103; Luciferase_bac/NFP.
DR   PRINTS; PR00089; LUCIFERASE.
DR   SUPFAM; SSF51679; SSF51679; 1.
PE   3: Inferred from homology;
KW   Flavoprotein; FMN; Luminescence; Monooxygenase; Oxidoreductase;
KW   Photoprotein.
FT   CHAIN           <1..>103
FT                   /note="Alkanal monooxygenase alpha chain"
FT                   /id="PRO_0000220170"
FT   VARIANT         101
FT                   /note="Y -> F (in strain: UM4082)"
FT   VARIANT         101
FT                   /note="Y -> S (in strain: UM4102)"
FT   NON_TER         1
FT   NON_TER         103
SQ   SEQUENCE   103 AA;  12337 MW;  D0DE5D3EEAB137D2 CRC64;
     INTHEKKAQL DLYNEIALEH GHDIQNIDHC LSYITSVDHD SQRAKDICRQ FLAHWYDSYV
     NATRIFDDSD QTKGYDFNKG QWRDFVLKGH RDTNRRIDYS YEI