ID   LUXB1_PHOLU             Reviewed;         327 AA.
AC   P19840;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=Alkanal monooxygenase beta chain;
DE            EC=1.14.14.3 {ECO:0000269|PubMed:2204626};
DE   AltName: Full=Bacterial luciferase beta chain;
GN   Name=luxB;
OS   Photorhabdus luminescens (Xenorhabdus luminescens).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Morganellaceae; Photorhabdus.
OX   NCBI_TaxID=29488;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND CATALYTIC ACTIVITY.
RC   STRAIN=ATCC 29999 / DSM 3368 / BCRC 14801 / CIP 106429 / NCIMB 12670 / Hb;
RX   PubMed=2204626; DOI=10.1016/s0021-9258(17)46262-4;
RA   Szittner R., Meighen E.;
RT   "Nucleotide sequence, expression, and properties of luciferase coded by lux
RT   genes from a terrestrial bacterium.";
RL   J. Biol. Chem. 265:16581-16587(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 29999 / DSM 3368 / BCRC 14801 / CIP 106429 / NCIMB 12670 / Hb;
RX   PubMed=1644764; DOI=10.1128/jb.174.16.5371-5381.1992;
RA   Meighen E.A., Szittner R.B.;
RT   "Multiple repetitive elements and organization of the lux operons of
RT   luminescent terrestrial bacteria.";
RL   J. Bacteriol. 174:5371-5381(1992).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Hm;
RX   PubMed=2383248; DOI=10.1016/0006-291x(90)92106-a;
RA   Johnston T.C., Rucker E.B., Cochrum L., Hruska K.S., Vandegrift V.;
RT   "The nucleotide sequence of the luxA and luxB genes of Xenorhabdus
RT   luminescens HM and a comparison of the amino acid sequences of luciferases
RT   from four species of bioluminescent bacteria.";
RL   Biochem. Biophys. Res. Commun. 170:407-415(1990).
CC   -!- FUNCTION: Light-emitting reaction in luminous bacteria. The specific
CC       role of the beta subunit is unknown, but it is absolutely required for
CC       bioluminescence activity. {ECO:0000269|PubMed:2204626}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a long-chain fatty aldehyde + FMNH2 + O2 = a long-chain fatty
CC         acid + FMN + 2 H(+) + H2O + hnu; Xref=Rhea:RHEA:17181,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:17176, ChEBI:CHEBI:30212, ChEBI:CHEBI:57560,
CC         ChEBI:CHEBI:57618, ChEBI:CHEBI:58210; EC=1.14.14.3;
CC         Evidence={ECO:0000269|PubMed:2204626};
CC   -!- SUBUNIT: Heterodimer of an alpha and a beta chain.
CC       {ECO:0000250|UniProtKB:P07740}.
CC   -!- SIMILARITY: Belongs to the bacterial luciferase oxidoreductase family.
CC       {ECO:0000305}.
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DR   EMBL; M57416; AAA27624.1; -; Genomic_DNA.
DR   EMBL; M90093; AAA27620.1; -; Genomic_DNA.
DR   EMBL; M55977; AAA27627.1; -; Genomic_DNA.
DR   PIR; C35411; C35411.
DR   AlphaFoldDB; P19840; -.
DR   SMR; P19840; -.
DR   STRING; 29488.KS18_21625; -.
DR   KEGG; ag:AAA27624; -.
DR   BRENDA; 1.14.14.3; 4782.
DR   GO; GO:0047646; F:alkanal monooxygenase (FMN-linked) activity; IEA:UniProtKB-EC.
DR   GO; GO:0008218; P:bioluminescence; IEA:UniProtKB-KW.
DR   CDD; cd01096; Alkanal_monooxygenase; 1.
DR   Gene3D; 3.20.20.30; -; 2.
DR   InterPro; IPR033924; Alkanal_monooxygenase.
DR   InterPro; IPR018235; Bacterial_luciferase_CS.
DR   InterPro; IPR011251; Luciferase-like_dom.
DR   InterPro; IPR036661; Luciferase-like_sf.
DR   InterPro; IPR002103; Luciferase_bac/NFP.
DR   Pfam; PF00296; Bac_luciferase; 1.
DR   PRINTS; PR00089; LUCIFERASE.
DR   SUPFAM; SSF51679; SSF51679; 1.
DR   PROSITE; PS00494; BACTERIAL_LUCIFERASE; 1.
PE   1: Evidence at protein level;
KW   Flavoprotein; FMN; Luminescence; Monooxygenase; Oxidoreductase;
KW   Photoprotein.
FT   CHAIN           1..327
FT                   /note="Alkanal monooxygenase beta chain"
FT                   /id="PRO_0000220177"
FT   VARIANT         85
FT                   /note="A -> R (in strain: Hm)"
SQ   SEQUENCE   327 AA;  37595 MW;  1905AF01658BE56E CRC64;
     MKFGLFFLNF INSTTVQEQS IVRMQEITEY VDKLNFEQIL VYENHFSDNG VVGAPLTVSG
     FLLGLTEKIK IGSLNHIITT HHPVAIAEEA CLLDQLSEGR FILGFSDCEK KDEMHFFNRP
     VEYQQQLFEE CYEIINDALT TGYCNPDNDF YSFPKISVNP HAYTPGGPRK YVTATSHHIV
     EWAAKKGIPL IFKWDDSNDV RYEYAERYKA VADKYDVDLS EIDHQLMILV NYNEDSNKAK
     QETRAFISDY VLEMHPNENF ENKLEEIIAE NAVGNYTECI TAAKLAIEKC GAKSVLLSFE
     PMNDLMSQKN VINIVDDNIK KYHMEYT