ID   LUXB2_PHOLU             Reviewed;         324 AA.
AC   P23147;
DT   01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1991, sequence version 1.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Alkanal monooxygenase beta chain;
DE            EC=1.14.14.3 {ECO:0000269|PubMed:1995589};
DE   AltName: Full=Bacterial luciferase beta chain;
GN   Name=luxB;
OS   Photorhabdus luminescens (Xenorhabdus luminescens).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Morganellaceae; Photorhabdus.
OX   NCBI_TaxID=29488;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND CATALYTIC ACTIVITY.
RC   STRAIN=Hw;
RX   PubMed=1995589; DOI=10.1128/jb.173.4.1399-1405.1991;
RA   Xi L., Cho K.W., Tu S.C.;
RT   "Cloning and nucleotide sequences of lux genes and characterization of
RT   luciferase of Xenorhabdus luminescens from a human wound.";
RL   J. Bacteriol. 173:1399-1405(1991).
CC   -!- FUNCTION: Light-emitting reaction in luminous bacteria. The specific
CC       role of the beta subunit is unknown, but it is absolutely required for
CC       bioluminescence activity. {ECO:0000269|PubMed:1995589}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a long-chain fatty aldehyde + FMNH2 + O2 = a long-chain fatty
CC         acid + FMN + 2 H(+) + H2O + hnu; Xref=Rhea:RHEA:17181,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:17176, ChEBI:CHEBI:30212, ChEBI:CHEBI:57560,
CC         ChEBI:CHEBI:57618, ChEBI:CHEBI:58210; EC=1.14.14.3;
CC         Evidence={ECO:0000269|PubMed:1995589};
CC   -!- SUBUNIT: Heterodimer of an alpha and a beta chain.
CC       {ECO:0000250|UniProtKB:P07740}.
CC   -!- SIMILARITY: Belongs to the bacterial luciferase oxidoreductase family.
CC       {ECO:0000305}.
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DR   EMBL; M62917; AAA63566.1; -; Genomic_DNA.
DR   AlphaFoldDB; P23147; -.
DR   SMR; P23147; -.
DR   GO; GO:0047646; F:alkanal monooxygenase (FMN-linked) activity; IEA:UniProtKB-EC.
DR   GO; GO:0008218; P:bioluminescence; IEA:UniProtKB-KW.
DR   CDD; cd01096; Alkanal_monooxygenase; 1.
DR   Gene3D; 3.20.20.30; -; 2.
DR   InterPro; IPR033924; Alkanal_monooxygenase.
DR   InterPro; IPR018235; Bacterial_luciferase_CS.
DR   InterPro; IPR011251; Luciferase-like_dom.
DR   InterPro; IPR036661; Luciferase-like_sf.
DR   InterPro; IPR002103; Luciferase_bac/NFP.
DR   Pfam; PF00296; Bac_luciferase; 1.
DR   PRINTS; PR00089; LUCIFERASE.
DR   SUPFAM; SSF51679; SSF51679; 1.
DR   PROSITE; PS00494; BACTERIAL_LUCIFERASE; 1.
PE   1: Evidence at protein level;
KW   Flavoprotein; FMN; Luminescence; Monooxygenase; Oxidoreductase;
KW   Photoprotein.
FT   CHAIN           1..324
FT                   /note="Alkanal monooxygenase beta chain"
FT                   /id="PRO_0000220178"
SQ   SEQUENCE   324 AA;  37115 MW;  E7D81324D344A975 CRC64;
     MKFGLFFLNF INSTTIQEQS IARMQEITEY VDKLNFEQIL VCENHFSDNG VVGAPLTVSG
     FLLGLTEKIK IGSLNHVITT HHPVRIAEEA CLLDQLSEGR FILGFSDCER KDEMPFFNRP
     EQYQQQLFEE CYDIINDALT TGYCNPNGDF YNFPKISMNP HAYTQNGPRK YVTATSCHVV
     EWAAKKGIPL IFKWDDSNEV KHEYAKRYQA IAGEYGVDLA EIDHQLMILV NYSEDSEKAK
     EETRAFISDY ILAMHPNENF EKKLEEIITE NSVGDYMECT TAAKLAMEKC GTKGILLSFE
     SMSDFTHQIN AIDIVNDNIK KYHM