ID   LUXB_PHOLL              Reviewed;         324 AA.
AC   Q7N574;
DT   10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Alkanal monooxygenase beta chain;
DE            EC=1.14.14.3 {ECO:0000250|UniProtKB:P19840};
DE   AltName: Full=Bacterial luciferase beta chain;
GN   Name=luxB; OrderedLocusNames=plu2082;
OS   Photorhabdus laumondii subsp. laumondii (strain DSM 15139 / CIP 105565 /
OS   TT01).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Morganellaceae; Photorhabdus.
OX   NCBI_TaxID=243265;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 15139 / CIP 105565 / TT01;
RX   PubMed=14528314; DOI=10.1038/nbt886;
RA   Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A.,
RA   Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F.,
RA   Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S., Medigue C.,
RA   Lanois A., Powell K., Siguier P., Vincent R., Wingate V., Zouine M.,
RA   Glaser P., Boemare N., Danchin A., Kunst F.;
RT   "The genome sequence of the entomopathogenic bacterium Photorhabdus
RT   luminescens.";
RL   Nat. Biotechnol. 21:1307-1313(2003).
CC   -!- FUNCTION: Light-emitting reaction in luminous bacteria. The specific
CC       role of the beta subunit is unknown, but it is absolutely required for
CC       bioluminescence activity. {ECO:0000250|UniProtKB:P19840}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a long-chain fatty aldehyde + FMNH2 + O2 = a long-chain fatty
CC         acid + FMN + 2 H(+) + H2O + hnu; Xref=Rhea:RHEA:17181,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:17176, ChEBI:CHEBI:30212, ChEBI:CHEBI:57560,
CC         ChEBI:CHEBI:57618, ChEBI:CHEBI:58210; EC=1.14.14.3;
CC         Evidence={ECO:0000250|UniProtKB:P19840};
CC   -!- SUBUNIT: Heterodimer of an alpha and a beta chain.
CC       {ECO:0000250|UniProtKB:P07740}.
CC   -!- SIMILARITY: Belongs to the bacterial luciferase oxidoreductase family.
CC       {ECO:0000305}.
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DR   EMBL; BX571866; CAE14375.1; -; Genomic_DNA.
DR   RefSeq; WP_011146337.1; NC_005126.1.
DR   AlphaFoldDB; Q7N574; -.
DR   SMR; Q7N574; -.
DR   STRING; 243265.plu2082; -.
DR   PRIDE; Q7N574; -.
DR   EnsemblBacteria; CAE14375; CAE14375; plu2082.
DR   GeneID; 24166557; -.
DR   KEGG; plu:plu2082; -.
DR   eggNOG; COG2141; Bacteria.
DR   HOGENOM; CLU_027853_3_0_6; -.
DR   OMA; WVDLEVF; -.
DR   OrthoDB; 1434838at2; -.
DR   BioCyc; PLUM243265:PLU_RS10400-MON; -.
DR   Proteomes; UP000002514; Chromosome.
DR   GO; GO:0047646; F:alkanal monooxygenase (FMN-linked) activity; IEA:UniProtKB-EC.
DR   GO; GO:0008218; P:bioluminescence; IEA:UniProtKB-KW.
DR   CDD; cd01096; Alkanal_monooxygenase; 1.
DR   Gene3D; 3.20.20.30; -; 2.
DR   InterPro; IPR033924; Alkanal_monooxygenase.
DR   InterPro; IPR018235; Bacterial_luciferase_CS.
DR   InterPro; IPR011251; Luciferase-like_dom.
DR   InterPro; IPR036661; Luciferase-like_sf.
DR   InterPro; IPR002103; Luciferase_bac/NFP.
DR   Pfam; PF00296; Bac_luciferase; 1.
DR   PRINTS; PR00089; LUCIFERASE.
DR   SUPFAM; SSF51679; SSF51679; 1.
DR   PROSITE; PS00494; BACTERIAL_LUCIFERASE; 1.
PE   3: Inferred from homology;
KW   Flavoprotein; FMN; Luminescence; Monooxygenase; Oxidoreductase;
KW   Photoprotein; Reference proteome.
FT   CHAIN           1..324
FT                   /note="Alkanal monooxygenase beta chain"
FT                   /id="PRO_0000220176"
SQ   SEQUENCE   324 AA;  37291 MW;  A5EA280507019967 CRC64;
     MKFGLFFLNF INSTTVQEQS IVRMQEITEY VDKLNFEQIL VYENHFSGNG VVGAPLTVSG
     FLLGLTEKIK IGSLNHIITT HHPVRIAEEA CLLDQLSEGR FILGFSDCEK KDEMRLFNRP
     VEYQQQLFEE CYEIINDALT TGYCNPDNDF YSFPKISVNP HAYTQGGPRR YVTATSHHIV
     EWAAKKGIPL IFKWDDSNDV RYEYAERYKA VADKYGIDLS AIDHQLMVLV NYNEDSHKAK
     QETRAFIRDY VLEMYPNENL ENKLEEIITE NAVGDYTECI AAAKLAIEKC GAKSVLLSFE
     PMNDLMHQKN VINIVNDNIK KYHM