LUXB_VIBHA
ID LUXB_VIBHA Reviewed; 324 AA.
AC P07739;
DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 2.
DT 25-MAY-2022, entry version 114.
DE RecName: Full=Alkanal monooxygenase beta chain;
DE EC=1.14.14.3 {ECO:0000250|UniProtKB:P19840};
DE AltName: Full=Bacterial luciferase beta chain;
GN Name=luxB;
OS Vibrio harveyi (Beneckea harveyi).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=669;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC STRAIN=ATCC 33843 / NCIMB 1871 / 392 / MAV;
RX PubMed=3514602; DOI=10.1016/s0021-9258(19)89176-7;
RA Johnston T.C., Thompson R.B., Baldwin T.O.;
RT "Nucleotide sequence of the luxB gene of Vibrio harveyi and the complete
RT amino acid sequence of the beta subunit of bacterial luciferase.";
RL J. Biol. Chem. 261:4805-4811(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-12.
RX PubMed=3997817; DOI=10.1016/s0021-9258(18)88948-7;
RA Cohn D.H., Mileham A.J., Simon M.I., Nealson K.H., Rausch S.K., Bonam D.,
RA Baldwin T.O.;
RT "Nucleotide sequence of the luxA gene of Vibrio harveyi and the complete
RT amino acid sequence of the alpha subunit of bacterial luciferase.";
RL J. Biol. Chem. 260:6139-6146(1985).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS), AND SUBUNIT.
RX PubMed=7756289; DOI=10.1021/bi00020a002;
RA Fisher A.J., Raushel F.M., Baldwin T.O., Rayment I.;
RT "Three-dimensional structure of bacterial luciferase from Vibrio harveyi at
RT 2.4-A resolution.";
RL Biochemistry 34:6581-6586(1995).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS), AND SUBUNIT.
RX PubMed=8703001; DOI=10.1074/jbc.271.36.21956;
RA Fisher A.J., Thompson T.B., Thoden J.B., Baldwin T.O., Rayment I.;
RT "The 1.5-A resolution crystal structure of bacterial luciferase in low salt
RT conditions.";
RL J. Biol. Chem. 271:21956-21968(1996).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
RX PubMed=9020763; DOI=10.1021/bi962511x;
RA Tanner J.J., Miller M.D., Wilson K.S., Tu S.C., Krause K.L.;
RT "Structure of bacterial luciferase beta 2 homodimer: implications for
RT flavin binding.";
RL Biochemistry 36:665-672(1997).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS).
RX PubMed=9007973; DOI=10.1002/pro.5560060103;
RA Thoden J.B., Holden H.M., Fisher A.J., Sinclair J.F., Wesenberg G.,
RA Baldwin T.O., Rayment I.;
RT "Structure of the beta 2 homodimer of bacterial luciferase from Vibrio
RT harveyi: X-ray analysis of a kinetic protein folding trap.";
RL Protein Sci. 6:13-23(1997).
CC -!- FUNCTION: Light-emitting reaction in luminous bacteria. The specific
CC role of the beta subunit is unknown, but it is absolutely required for
CC bioluminescence activity. {ECO:0000250|UniProtKB:P19840}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a long-chain fatty aldehyde + FMNH2 + O2 = a long-chain fatty
CC acid + FMN + 2 H(+) + H2O + hnu; Xref=Rhea:RHEA:17181,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:17176, ChEBI:CHEBI:30212, ChEBI:CHEBI:57560,
CC ChEBI:CHEBI:57618, ChEBI:CHEBI:58210; EC=1.14.14.3;
CC Evidence={ECO:0000250|UniProtKB:P19840};
CC -!- SUBUNIT: Heterodimer of an alpha and a beta chain.
CC {ECO:0000269|PubMed:7756289, ECO:0000269|PubMed:8703001}.
CC -!- SIMILARITY: Belongs to the bacterial luciferase oxidoreductase family.
CC {ECO:0000305}.
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DR EMBL; M10961; AAA88686.1; -; Genomic_DNA.
DR PIR; A23866; A23866.
DR RefSeq; WP_010991820.1; NZ_UAVF01000022.1.
DR PDB; 1BRL; X-ray; 2.40 A; B/D=1-324.
DR PDB; 1BSL; X-ray; 1.95 A; A/B=1-324.
DR PDB; 1LUC; X-ray; 1.50 A; B=1-324.
DR PDB; 1XKJ; X-ray; 2.50 A; A/B=1-324.
DR PDB; 3FGC; X-ray; 2.30 A; B/D=1-324.
DR PDBsum; 1BRL; -.
DR PDBsum; 1BSL; -.
DR PDBsum; 1LUC; -.
DR PDBsum; 1XKJ; -.
DR PDBsum; 3FGC; -.
DR AlphaFoldDB; P07739; -.
DR SMR; P07739; -.
DR MINT; P07739; -.
DR GeneID; 57823102; -.
DR BRENDA; 1.14.14.3; 4778.
DR EvolutionaryTrace; P07739; -.
DR GO; GO:0047646; F:alkanal monooxygenase (FMN-linked) activity; IEA:UniProtKB-EC.
DR GO; GO:0008218; P:bioluminescence; IEA:UniProtKB-KW.
DR CDD; cd01096; Alkanal_monooxygenase; 1.
DR Gene3D; 3.20.20.30; -; 2.
DR InterPro; IPR033924; Alkanal_monooxygenase.
DR InterPro; IPR018235; Bacterial_luciferase_CS.
DR InterPro; IPR011251; Luciferase-like_dom.
DR InterPro; IPR036661; Luciferase-like_sf.
DR InterPro; IPR002103; Luciferase_bac/NFP.
DR Pfam; PF00296; Bac_luciferase; 1.
DR PRINTS; PR00089; LUCIFERASE.
DR SUPFAM; SSF51679; SSF51679; 1.
DR PROSITE; PS00494; BACTERIAL_LUCIFERASE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Flavoprotein; FMN; Luminescence;
KW Monooxygenase; Oxidoreductase; Photoprotein.
FT CHAIN 1..324
FT /note="Alkanal monooxygenase beta chain"
FT /id="PRO_0000220181"
FT STRAND 2..7
FT /evidence="ECO:0007829|PDB:1LUC"
FT STRAND 13..15
FT /evidence="ECO:0007829|PDB:1LUC"
FT HELIX 17..32
FT /evidence="ECO:0007829|PDB:1LUC"
FT TURN 33..35
FT /evidence="ECO:0007829|PDB:1BRL"
FT STRAND 38..41
FT /evidence="ECO:0007829|PDB:1LUC"
FT STRAND 46..49
FT /evidence="ECO:0007829|PDB:1LUC"
FT HELIX 55..65
FT /evidence="ECO:0007829|PDB:1LUC"
FT STRAND 67..77
FT /evidence="ECO:0007829|PDB:1LUC"
FT HELIX 78..80
FT /evidence="ECO:0007829|PDB:1XKJ"
FT HELIX 83..96
FT /evidence="ECO:0007829|PDB:1LUC"
FT STRAND 101..106
FT /evidence="ECO:0007829|PDB:1LUC"
FT HELIX 111..116
FT /evidence="ECO:0007829|PDB:1LUC"
FT HELIX 121..123
FT /evidence="ECO:0007829|PDB:1LUC"
FT HELIX 124..141
FT /evidence="ECO:0007829|PDB:1LUC"
FT STRAND 142..144
FT /evidence="ECO:0007829|PDB:1LUC"
FT STRAND 147..150
FT /evidence="ECO:0007829|PDB:1LUC"
FT STRAND 155..157
FT /evidence="ECO:0007829|PDB:1BSL"
FT STRAND 170..173
FT /evidence="ECO:0007829|PDB:1LUC"
FT HELIX 177..186
FT /evidence="ECO:0007829|PDB:1LUC"
FT STRAND 190..192
FT /evidence="ECO:0007829|PDB:1LUC"
FT HELIX 198..214
FT /evidence="ECO:0007829|PDB:1LUC"
FT HELIX 219..221
FT /evidence="ECO:0007829|PDB:1BSL"
FT STRAND 225..232
FT /evidence="ECO:0007829|PDB:1LUC"
FT HELIX 236..254
FT /evidence="ECO:0007829|PDB:1LUC"
FT TURN 255..257
FT /evidence="ECO:0007829|PDB:1BSL"
FT HELIX 260..270
FT /evidence="ECO:0007829|PDB:1LUC"
FT STRAND 271..275
FT /evidence="ECO:0007829|PDB:1LUC"
FT HELIX 276..290
FT /evidence="ECO:0007829|PDB:1LUC"
FT STRAND 293..298
FT /evidence="ECO:0007829|PDB:1LUC"
FT HELIX 305..317
FT /evidence="ECO:0007829|PDB:1LUC"
FT HELIX 319..324
FT /evidence="ECO:0007829|PDB:3FGC"
SQ SEQUENCE 324 AA; 36345 MW; 974FF37653C052D3 CRC64;
MKFGLFFLNF MNSKRSSDQV IEEMLDTAHY VDQLKFDTLA VYENHFSNNG VVGAPLTVAG
FLLGMTKNAK VASLNHVITT HHPVRVAEEA CLLDQMSEGR FAFGFSDCEK SADMRFFNRP
TDSQFQLFSE CHKIINDAFT TGYCHPNNDF YSFPKISVNP HAFTEGGPAQ FVNATSKEVV
EWAAKLGLPL VFRWDDSNAQ RKEYAGLYHE VAQAHGVDVS QVRHKLTLLV NQNVDGEAAR
AEARVYLEEF VRESYSNTDF EQKMGELLSE NAIGTYEEST QAARVAIECC GAADLLMSFE
SMEDKAQQRA VIDVVNANIV KYHS
