ID   LUXO_VIBC3              Reviewed;         530 AA.
AC   O87455; A5EYL5; C3M7M0;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   26-FEB-2008, sequence version 2.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=Regulatory protein LuxO;
GN   Name=luxO; Synonyms=s54act4; OrderedLocusNames=VC0395_1095, VC395_A0173;
OS   Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 /
OS   O395).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=345073;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RA   Heidelberg J.;
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RX   PubMed=19115014; DOI=10.1371/journal.pone.0004053;
RA   Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J.,
RA   Wang W., Wang J., Qian W., Li D., Wang L.;
RT   "A recalibrated molecular clock and independent origins for the cholera
RT   pandemic clones.";
RL   PLoS ONE 3:E4053-E4053(2008).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 262-411, AND INTERACTION WITH
RP   SIGMA-54.
RX   PubMed=9748465; DOI=10.1128/jb.180.19.5256-5259.1998;
RA   Klose K.E., Novik V., Mekalanos J.J.;
RT   "Identification of multiple sigma54-dependent transcriptional activators in
RT   Vibrio cholerae.";
RL   J. Bacteriol. 180:5256-5259(1998).
CC   -!- FUNCTION: Involved in the regulation of different processes depending
CC       on the cell density. Acts together with sigma-54 to repress, perhaps
CC       indirectly, some genes.
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DR   EMBL; CP000626; ABQ18923.1; -; Genomic_DNA.
DR   EMBL; CP001236; ACP11016.1; -; Genomic_DNA.
DR   EMBL; AF069388; AAC62403.1; -; Genomic_DNA.
DR   RefSeq; WP_001155015.1; NZ_JAACZH010000004.1.
DR   AlphaFoldDB; O87455; -.
DR   SMR; O87455; -.
DR   STRING; 345073.VC395_A0173; -.
DR   EnsemblBacteria; ABQ18923; ABQ18923; VC0395_1095.
DR   GeneID; 57741635; -.
DR   KEGG; vco:VC0395_1095; -.
DR   KEGG; vcr:VC395_A0173; -.
DR   PATRIC; fig|345073.21.peg.2934; -.
DR   eggNOG; COG3604; Bacteria.
DR   HOGENOM; CLU_000445_125_3_6; -.
DR   OMA; HLEVHDC; -.
DR   Proteomes; UP000000249; Chromosome 1.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 3.30.450.40; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003018; GAF.
DR   InterPro; IPR029016; GAF-like_dom_sf.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002078; Sigma_54_int.
DR   InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR   InterPro; IPR025943; Sigma_54_int_dom_ATP-bd_2.
DR   InterPro; IPR025944; Sigma_54_int_dom_CS.
DR   Pfam; PF01590; GAF; 1.
DR   Pfam; PF00158; Sigma54_activat; 1.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00065; GAF; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR   PROSITE; PS00676; SIGMA54_INTERACT_2; 1.
DR   PROSITE; PS00688; SIGMA54_INTERACT_3; 1.
DR   PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; DNA-binding; Nucleotide-binding; Phosphoprotein; Repressor;
KW   Transcription; Transcription regulation; Two-component regulatory system.
FT   CHAIN           1..530
FT                   /note="Regulatory protein LuxO"
FT                   /id="PRO_0000081116"
FT   DOMAIN          194..423
FT                   /note="Sigma-54 factor interaction"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   BINDING         222..229
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   BINDING         285..294
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   CONFLICT        407
FT                   /note="W -> C (in Ref. 3; AAC62403)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   530 AA;  59027 MW;  5B85E12F25A03FA2 CRC64;
     MQEFSLSTLL EMTVGLASGA NNEERFHRLL DAVRKAVICD CVVLMSLHND TLTPLAMQGL
     TRDTLGRRFV VSEHPRLAQI CSADLPVRFA ADCPLPDPFD GLLLDSEDDL PMHSCMGLPL
     HFGEQLLGIL TLDSLKPDAF DHLSPRSLEI LAAIAAATLK MTLTFSELEN QAKQTQLRLE
     ELNQEAWSRD SVEIIGNSGP MLAMKADIDV VAPSQFNILI HGETGVGKEL VARTIHQRSN
     RKRQPLVYVN CAAIPENLLE SELFGHVKGA FTGADRARMG KFALADGGTL FLDEIGELPL
     SAQSKILRAL QNHEIQPVGQ DRVQTVDVRI LAATNRDLKK EVEAGRFRAD LYHRLSVYPI
     YVPPLRERKG DLSLLAGYFV EQARRKLGIT QLKLHGDVLS QLIQYPWPGN VRELEHVINR
     AALKAKARQR GRPVTTLKVE DLGELQGPRA AMVEPTAQDE PMLGEWIGEL GLRDATDEFQ
     RHLISETLTQ ADFNWAEAAR RLQTDRANLT RLAKRLGITV SRSHSIERSR