ID   LUXO_VIBPA              Reviewed;         453 AA.
AC   Q87MX7;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 117.
DE   RecName: Full=Regulatory protein LuxO;
GN   Name=luxO; OrderedLocusNames=VP2099;
OS   Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=223926;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RIMD 2210633;
RX   PubMed=12620739; DOI=10.1016/s0140-6736(03)12659-1;
RA   Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K.,
RA   Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S.,
RA   Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T.;
RT   "Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism
RT   distinct from that of V. cholerae.";
RL   Lancet 361:743-749(2003).
CC   -!- FUNCTION: Involved in the regulation of different processes depending
CC       on the cell density. Acts together with sigma-54 to repress, perhaps
CC       indirectly, some genes (By similarity). {ECO:0000250}.
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DR   EMBL; BA000031; BAC60362.1; -; Genomic_DNA.
DR   RefSeq; NP_798478.1; NC_004603.1.
DR   AlphaFoldDB; Q87MX7; -.
DR   SMR; Q87MX7; -.
DR   STRING; 223926.28807092; -.
DR   EnsemblBacteria; BAC60362; BAC60362; BAC60362.
DR   KEGG; vpa:VP2099; -.
DR   PATRIC; fig|223926.6.peg.2010; -.
DR   eggNOG; COG2204; Bacteria.
DR   HOGENOM; CLU_000445_0_6_6; -.
DR   OMA; MPISMQV; -.
DR   Proteomes; UP000002493; Chromosome 1.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR002197; HTH_Fis.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR002078; Sigma_54_int.
DR   InterPro; IPR025943; Sigma_54_int_dom_ATP-bd_2.
DR   InterPro; IPR025944; Sigma_54_int_dom_CS.
DR   Pfam; PF02954; HTH_8; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   Pfam; PF00158; Sigma54_activat; 1.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00448; REC; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
DR   PROSITE; PS00676; SIGMA54_INTERACT_2; 1.
DR   PROSITE; PS00688; SIGMA54_INTERACT_3; 1.
DR   PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA-binding; Nucleotide-binding; Phosphoprotein;
KW   Reference proteome; Repressor; Transcription; Transcription regulation;
KW   Two-component regulatory system.
FT   CHAIN           1..453
FT                   /note="Regulatory protein LuxO"
FT                   /id="PRO_0000081118"
FT   DOMAIN          1..112
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DOMAIN          133..362
FT                   /note="Sigma-54 factor interaction"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   BINDING         161..168
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   BINDING         224..233
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   MOD_RES         47
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ   SEQUENCE   453 AA;  50482 MW;  A0A9CE20D91B4AFF CRC64;
     MVEDTASVAA LYRSYLTPLG IDINIVGTGR DAIESLNHRI PDLILLDLRL PDMTGMDVLH
     AVKKSHPDVP IIFMTAHGSI DTAVEAMRHG SQDFLIKPCE ADRLRVTVNN AIRKATKLKN
     EADNPGNQNY QGFIGSSQTM QQVYRTIDSA ASSKASIFIT GESGTGKEVC AEAIHAASKR
     GDKPFIAINC AAIPKDLIES ELFGHVKGAF TGAANDRQGA AELADGGTLF LDELCEMDLD
     LQTKLLRFIQ TGTFQKVGSS KMKSVDVRFV CATNRDPWKE VQEGRFREDL YYRLYVIPLH
     LPPLRERGED VIEIAYSLLG YMSHEEGKNF VRFSQEVIDR FNSYEWPGNV RQLQNVLRNI
     VVLNNGKEIT LDMLPPPLNQ PLDRPSVSKL IEPKAMTVSE IMPLWMTEKM AIEQAIEACD
     GNIPRAAGYL DVSPSTIYRK LQAWNGKEER QKV