LUXR_ALIFS
ID LUXR_ALIFS Reviewed; 250 AA.
AC P12746;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 3.
DT 25-MAY-2022, entry version 112.
DE RecName: Full=Transcriptional activator protein LuxR;
GN Name=luxR;
OS Aliivibrio fischeri (Vibrio fischeri).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Aliivibrio.
OX NCBI_TaxID=668;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 7744 / DSM 507 / NCIMB 1281 / 398;
RA Devine J.H., Countryman C., Baldwin T.O.;
RT "Nucleotide sequence of the luxR and luxI genes and structure of the
RT primary regulatory region of the lux regulon of Vibrio fischeri ATCC
RT 7744.";
RL Biochemistry 27:837-842(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SEQUENCE REVISION.
RC STRAIN=ATCC 7744 / DSM 507 / NCIMB 1281 / 398;
RX PubMed=2762291; DOI=10.1073/pnas.86.15.5688;
RA Devine J.H., Shadel G.S., Baldwin T.O.;
RT "Identification of the operator of the lux regulon from the Vibrio fischeri
RT strain ATCC7744.";
RL Proc. Natl. Acad. Sci. U.S.A. 86:5688-5692(1989).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=MJ-1;
RX PubMed=3697093; DOI=10.1093/nar/15.24.10455;
RA Engebrecht J., Silverman M.;
RT "Nucleotide sequence of the regulatory locus controlling expression of
RT bacterial genes for bioluminescence.";
RL Nucleic Acids Res. 15:10455-10467(1987).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=MJ-1;
RA Knight T., Papadakis N.;
RT "Vibrio fischeri Lux operon SalI digest.";
RL Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP PROTEIN SEQUENCE OF 1-15, AND DNA-BINDING.
RX PubMed=16578817; DOI=10.1073/pnas.84.19.6639;
RA Kaplan H.B., Greenberg E.P.;
RT "Overproduction and purification of the luxR gene product: transcriptional
RT activator of the Vibrio fischeri luminescence system.";
RL Proc. Natl. Acad. Sci. U.S.A. 84:6639-6643(1987).
CC -!- FUNCTION: Transcriptional activator of the bioluminescence operon.
CC Binds to the OHHL autoinducer.
CC -!- SIMILARITY: Belongs to the autoinducer-regulated transcriptional
CC regulatory protein family. {ECO:0000305}.
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DR EMBL; M25751; AAA27540.1; -; Genomic_DNA.
DR EMBL; M19039; AAA27551.1; -; Genomic_DNA.
DR EMBL; M25752; AAA27541.1; -; Genomic_DNA.
DR EMBL; AF170104; AAD48473.1; -; Genomic_DNA.
DR EMBL; Y00509; CAA68561.1; -; Genomic_DNA.
DR PIR; B33538; B33538.
DR PIR; S06314; S06314.
DR AlphaFoldDB; P12746; -.
DR SMR; P12746; -.
DR IntAct; P12746; 1.
DR BindingDB; P12746; -.
DR ChEMBL; CHEMBL5351; -.
DR DrugCentral; P12746; -.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0008218; P:bioluminescence; IEA:UniProtKB-KW.
DR GO; GO:0052043; P:modification by symbiont of host cellular component; IMP:CACAO.
DR GO; GO:1905087; P:positive regulation of bioluminescence; IMP:CACAO.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:CACAO.
DR GO; GO:0009372; P:quorum sensing; IEA:UniProtKB-KW.
DR CDD; cd06170; LuxR_C_like; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 3.30.450.80; -; 1.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR005143; TF_LuxR_autoind-bd_dom.
DR InterPro; IPR036693; TF_LuxR_autoind-bd_dom_sf.
DR InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF03472; Autoind_bind; 1.
DR Pfam; PF00196; GerE; 1.
DR PRINTS; PR00038; HTHLUXR.
DR SMART; SM00421; HTH_LUXR; 1.
DR SUPFAM; SSF46894; SSF46894; 1.
DR SUPFAM; SSF75516; SSF75516; 1.
DR PROSITE; PS00622; HTH_LUXR_1; 1.
DR PROSITE; PS50043; HTH_LUXR_2; 1.
PE 1: Evidence at protein level;
KW Activator; Direct protein sequencing; DNA-binding; Luminescence;
KW Quorum sensing; Transcription; Transcription regulation.
FT CHAIN 1..250
FT /note="Transcriptional activator protein LuxR"
FT /id="PRO_0000184161"
FT DOMAIN 176..241
FT /note="HTH luxR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT DNA_BIND 200..219
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT VARIANT 3
FT /note="N -> D (in strain: ATCC 7744)"
FT VARIANT 115
FT /note="T -> S (in strain: ATCC 7744)"
FT VARIANT 211
FT /note="E -> K (in strain: ATCC 7744)"
FT VARIANT 250
FT /note="N -> S (in strain: ATCC 7744)"
FT CONFLICT 21..23
FT /note="SNN -> AY (in Ref. 3; CAA68561)"
FT /evidence="ECO:0000305"
FT CONFLICT 42
FT /note="L -> LT (in Ref. 3; CAA68561)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 250 AA; 28556 MW; BDB636192F5E1C64 CRC64;
MKNINADDTY RIINKIKACR SNNDINQCLS DMTKMVHCEY YLLAIIYPHS MVKSDISILD
NYPKKWRQYY DDANLIKYDP IVDYSNSNHS PINWNIFENN AVNKKSPNVI KEAKTSGLIT
GFSFPIHTAN NGFGMLSFAH SEKDNYIDSL FLHACMNIPL IVPSLVDNYR KINIANNKSN
NDLTKREKEC LAWACEGKSS WDISKILGCS ERTVTFHLTN AQMKLNTTNR CQSISKAILT
GAIDCPYFKN
