LUXS_HELPJ
ID LUXS_HELPJ Reviewed; 152 AA.
AC Q9ZMW8;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=S-ribosylhomocysteine lyase;
DE EC=4.4.1.21;
DE AltName: Full=AI-2 synthesis protein;
DE AltName: Full=Autoinducer-2 production protein LuxS;
GN Name=luxS; OrderedLocusNames=jhp_0097;
OS Helicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99).
OC Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC Helicobacteraceae; Helicobacter.
OX NCBI_TaxID=85963;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=J99 / ATCC 700824;
RX PubMed=9923682; DOI=10.1038/16495;
RA Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C.,
RA Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J.,
RA Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D.,
RA Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J.;
RT "Genomic sequence comparison of two unrelated isolates of the human gastric
RT pathogen Helicobacter pylori.";
RL Nature 397:176-180(1999).
RN [2]
RP X-RAY CRYSTALLOGRAPHY (2.38 ANGSTROMS).
RX PubMed=11435117; DOI=10.1016/s0969-2126(01)00613-x;
RA Lewis H.A., Furlong E.B., Laubert B., Eroshkina G.A., Batiyenko Y.,
RA Adams J.M., Bergseid M.G., Marsh C.D., Peat T.S., Sanderson W.E.,
RA Sauder J.M., Buchanan S.G.;
RT "A structural genomics approach to the study of quorum sensing: crystal
RT structures of three LuxS orthologs.";
RL Structure 9:527-537(2001).
CC -!- FUNCTION: Involved in the synthesis of autoinducer 2 (AI-2) which is
CC secreted by bacteria and is used to communicate both the cell density
CC and the metabolic potential of the environment. The regulation of gene
CC expression in response to changes in cell density is called quorum
CC sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to
CC homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = (S)-4,5-
CC dihydroxypentane-2,3-dione + L-homocysteine; Xref=Rhea:RHEA:17753,
CC ChEBI:CHEBI:29484, ChEBI:CHEBI:58195, ChEBI:CHEBI:58199; EC=4.4.1.21;
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Note=Binds 1 Fe cation per subunit.;
CC -!- SUBUNIT: Homodimer.
CC -!- SIMILARITY: Belongs to the LuxS family. {ECO:0000305}.
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DR EMBL; AE001439; AAD05688.1; -; Genomic_DNA.
DR PIR; C71973; C71973.
DR RefSeq; WP_000783567.1; NZ_CP011330.1.
DR PDB; 1J6X; X-ray; 2.38 A; A/B=1-152.
DR PDBsum; 1J6X; -.
DR AlphaFoldDB; Q9ZMW8; -.
DR SMR; Q9ZMW8; -.
DR STRING; 85963.jhp_0097; -.
DR EnsemblBacteria; AAD05688; AAD05688; jhp_0097.
DR KEGG; hpj:jhp_0097; -.
DR PATRIC; fig|85963.30.peg.931; -.
DR eggNOG; COG1854; Bacteria.
DR OMA; GPMGCLT; -.
DR BRENDA; 4.4.1.21; 2604.
DR EvolutionaryTrace; Q9ZMW8; -.
DR Proteomes; UP000000804; Chromosome.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0043768; F:S-ribosylhomocysteine lyase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0009372; P:quorum sensing; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.1360.80; -; 1.
DR HAMAP; MF_00091; LuxS; 1.
DR InterPro; IPR037005; LuxS_sf.
DR InterPro; IPR011249; Metalloenz_LuxS/M16.
DR InterPro; IPR003815; S-ribosylhomocysteinase.
DR PANTHER; PTHR35799; PTHR35799; 1.
DR Pfam; PF02664; LuxS; 1.
DR PIRSF; PIRSF006160; AI2; 1.
DR PRINTS; PR01487; LUXSPROTEIN.
DR SUPFAM; SSF63411; SSF63411; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Autoinducer synthesis; Iron; Lyase; Metal-binding;
KW Quorum sensing.
FT CHAIN 1..152
FT /note="S-ribosylhomocysteine lyase"
FT /id="PRO_0000172230"
FT BINDING 55
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT BINDING 59
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT BINDING 122
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT HELIX 6..9
FT /evidence="ECO:0007829|PDB:1J6X"
FT STRAND 17..27
FT /evidence="ECO:0007829|PDB:1J6X"
FT STRAND 33..40
FT /evidence="ECO:0007829|PDB:1J6X"
FT TURN 44..46
FT /evidence="ECO:0007829|PDB:1J6X"
FT HELIX 51..68
FT /evidence="ECO:0007829|PDB:1J6X"
FT STRAND 72..77
FT /evidence="ECO:0007829|PDB:1J6X"
FT STRAND 81..90
FT /evidence="ECO:0007829|PDB:1J6X"
FT HELIX 94..108
FT /evidence="ECO:0007829|PDB:1J6X"
FT TURN 119..121
FT /evidence="ECO:0007829|PDB:1J6X"
FT TURN 123..126
FT /evidence="ECO:0007829|PDB:1J6X"
FT HELIX 130..142
FT /evidence="ECO:0007829|PDB:1J6X"
FT HELIX 144..146
FT /evidence="ECO:0007829|PDB:1J6X"
SQ SEQUENCE 152 AA; 17424 MW; B2E154CF36958315 CRC64;
MKMNVESFNL DHTKVKAPYV RIADRKKGVN GDLIVKYDVR FKQPNRDHMD MPSLHSLEHL
VAEIIRNHAN YVVDWSPMGC QTGFYLTVLN HDNYTEILEV LEKTMQDVLK AKEVPASNEK
QCGWAANHTL EGAQNLARAF LDKRAEWSEV GV
