ID   LUXY_ALIFS              Reviewed;         194 AA.
AC   P21578;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1991, sequence version 1.
DT   03-AUG-2022, entry version 84.
DE   RecName: Full=Yellow fluorescent protein;
DE            Short=YFP;
GN   Name=luxY;
OS   Aliivibrio fischeri (Vibrio fischeri).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Aliivibrio.
OX   NCBI_TaxID=668;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-15.
RC   STRAIN=ATCC 33715 / Y-1;
RX   PubMed=2201407; DOI=10.1021/bi00475a014;
RA   Baldwin T.O., Treat M.L., Daubner S.C.;
RT   "Cloning and expression of the luxY gene from Vibrio fischeri strain Y-1 in
RT   Escherichia coli and complete amino acid sequence of the yellow fluorescent
RT   protein.";
RL   Biochemistry 29:5509-5515(1990).
RN   [2]
RP   PROTEIN SEQUENCE OF 1-15, AND SUBUNIT.
RC   STRAIN=ATCC 33715 / Y-1;
RX   PubMed=3480518; DOI=10.1073/pnas.84.24.8912;
RA   Daubner S.C., Astorga A.M., Leisman G.B., Baldwin T.O.;
RT   "Yellow light emission of Vibrio fischeri strain Y-1: purification and
RT   characterization of the energy-accepting yellow fluorescent protein.";
RL   Proc. Natl. Acad. Sci. U.S.A. 84:8912-8916(1987).
CC   -!- FUNCTION: Antenna protein that modulates the color of the
CC       bioluminescence emission of the luciferase. In the presence of YFP and
CC       only at temperatures below 20 degrees Celsius, luciferase exhibits a
CC       bimodal emission spectrum with a new peak at 545 nM (yellow), in
CC       addition to the one at 485 nM.
CC   -!- COFACTOR:
CC       Name=FMN; Xref=ChEBI:CHEBI:58210;
CC       Note=Binds 1 FMN non-covalently.;
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:3480518}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M60852; AAA27544.1; -; Genomic_DNA.
DR   PIR; A36037; A36037.
DR   AlphaFoldDB; P21578; -.
DR   SMR; P21578; -.
DR   PRIDE; P21578; -.
DR   GO; GO:0008218; P:bioluminescence; IDA:CACAO.
DR   Gene3D; 2.40.30.20; -; 2.
DR   InterPro; IPR023366; ATP_synth_asu-like_sf.
DR   InterPro; IPR001783; Lumazine-bd.
DR   InterPro; IPR026017; Lumazine-bd_dom.
DR   InterPro; IPR017938; Riboflavin_synthase-like_b-brl.
DR   PANTHER; PTHR21098; PTHR21098; 1.
DR   Pfam; PF00677; Lum_binding; 2.
DR   PIRSF; PIRSF000498; Riboflavin_syn_A; 1.
DR   SUPFAM; SSF63380; SSF63380; 2.
DR   PROSITE; PS51177; LUMAZINE_BIND; 2.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Flavoprotein; FMN; Luminescence; Repeat.
FT   CHAIN           1..194
FT                   /note="Yellow fluorescent protein"
FT                   /id="PRO_0000068152"
FT   REPEAT          1..98
FT                   /note="Lumazine-binding 1"
FT   REPEAT          99..194
FT                   /note="Lumazine-binding 2"
FT   BINDING         179..183
FT                   /ligand="FMN"
FT                   /ligand_id="ChEBI:CHEBI:58210"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   194 AA;  22237 MW;  D28DCE6C81216FFB CRC64;
     MFKGIVEGIG IIEKIDIYTD LDKYAIRFPE NMLNGIKKES SIMFNGCFLT VTSVNSNIVW
     FDIFEKEARK LDTFREYKVG DRVNLGTFPK FGAASGGHIL SARISCVASI IEIIENEDYQ
     QMWIQIPENF TEFLIDKDYI AVDGISLTID TIKNNQFFIS LPLKIAQNTN MKWRKKGDKV
     NVELSNKINA NQCW