LUZP4_HUMAN
ID LUZP4_HUMAN Reviewed; 313 AA.
AC Q9P127; B3KSD6;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Leucine zipper protein 4;
DE AltName: Full=Cancer/testis antigen 28;
DE Short=CT-28;
DE Short=CT28;
DE AltName: Full=Tumor antigen HOM-TES-85;
GN Name=LUZP4;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RC TISSUE=Testis;
RX PubMed=12032826; DOI=10.1038/sj.onc.1205481;
RA Tuereci O., Sahin U., Koslowski M., Buss B., Bell C., Ballweber P.,
RA Zwick C., Eberle T., Zuber M., Villena-Heinsen C., Seitz G.,
RA Pfreundschuh M.;
RT "A novel tumour associated leucine zipper protein targeting to sites of
RT gene transcription and splicing.";
RL Oncogene 21:3879-3888(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15772651; DOI=10.1038/nature03440;
RA Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D.,
RA Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L.,
RA Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.,
RA Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A.,
RA Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P.,
RA Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D.,
RA Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D.,
RA Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L.,
RA Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P.,
RA Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G.,
RA Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J.,
RA Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D.,
RA Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L.,
RA Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z.,
RA Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S.,
RA Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S.,
RA Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O.,
RA Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H.,
RA Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T.,
RA Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L.,
RA Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R.,
RA Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y.,
RA Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K.,
RA Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J.,
RA Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L.,
RA Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S.,
RA Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A.,
RA Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L.,
RA Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D.,
RA Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H.,
RA McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S.,
RA Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C.,
RA Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S.,
RA Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V.,
RA Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K.,
RA Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K.,
RA Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D.,
RA Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R.,
RA Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B.,
RA Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C.,
RA d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q.,
RA Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N.,
RA Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A.,
RA Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J.,
RA Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A.,
RA Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F.,
RA Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L.,
RA Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S.,
RA Rogers J., Bentley D.R.;
RT "The DNA sequence of the human X chromosome.";
RL Nature 434:325-337(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-234, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH NXF1; NXF2; DDX39B; THOC1;
RP THOC5 AND SRRT, TISSUE SPECIFICITY, AND RNA-BINDING.
RX PubMed=25662211; DOI=10.1093/nar/gkv070;
RA Viphakone N., Cumberbatch M.G., Livingstone M.J., Heath P.R., Dickman M.J.,
RA Catto J.W., Wilson S.A.;
RT "Luzp4 defines a new mRNA export pathway in cancer cells.";
RL Nucleic Acids Res. 43:2353-2366(2015).
CC -!- FUNCTION: Export adapter involved in mRNA nuclear export in cancer
CC cells. Binds and enhances the RNA-binding activity of the nuclear RNA
CC export factor NXF1. Can restore mRNA export function in cells
CC compromised by loss of mRNA export adapters (PubMed:25662211).
CC {ECO:0000269|PubMed:25662211}.
CC -!- SUBUNIT: Interacts NXF1, NXF2, THOC1, THOC5, DDX39B/UAP56 and SRRT.
CC {ECO:0000269|PubMed:25662211}.
CC -!- INTERACTION:
CC Q9P127; Q92624: APPBP2; NbExp=6; IntAct=EBI-10198848, EBI-743771;
CC Q9P127; Q9BYV9: BACH2; NbExp=3; IntAct=EBI-10198848, EBI-1642333;
CC Q9P127; Q16204: CCDC6; NbExp=3; IntAct=EBI-10198848, EBI-1045350;
CC Q9P127; P49760: CLK2; NbExp=6; IntAct=EBI-10198848, EBI-750020;
CC Q9P127; Q13838: DDX39B; NbExp=3; IntAct=EBI-10198848, EBI-348622;
CC Q9P127; Q92997: DVL3; NbExp=3; IntAct=EBI-10198848, EBI-739789;
CC Q9P127; P50402: EMD; NbExp=9; IntAct=EBI-10198848, EBI-489887;
CC Q9P127; Q6FG41: FOS; NbExp=3; IntAct=EBI-10198848, EBI-10198738;
CC Q9P127; P15408: FOSL2; NbExp=3; IntAct=EBI-10198848, EBI-3893419;
CC Q9P127; O75031: HSF2BP; NbExp=3; IntAct=EBI-10198848, EBI-7116203;
CC Q9P127; P60370: KRTAP10-5; NbExp=3; IntAct=EBI-10198848, EBI-10172150;
CC Q9P127; P60410: KRTAP10-8; NbExp=3; IntAct=EBI-10198848, EBI-10171774;
CC Q9P127; P60411: KRTAP10-9; NbExp=3; IntAct=EBI-10198848, EBI-10172052;
CC Q9P127; Q8TBB1: LNX1; NbExp=3; IntAct=EBI-10198848, EBI-739832;
CC Q9P127; Q15014: MORF4L2; NbExp=3; IntAct=EBI-10198848, EBI-399257;
CC Q9P127; Q9P2K3: RCOR3; NbExp=3; IntAct=EBI-10198848, EBI-743428;
CC Q9P127; P78362: SRPK2; NbExp=3; IntAct=EBI-10198848, EBI-593303;
CC Q9P127; A7MD48: SRRM4; NbExp=3; IntAct=EBI-10198848, EBI-3867173;
CC Q9P127; Q9UKI8: TLK1; NbExp=3; IntAct=EBI-10198848, EBI-740492;
CC Q9P127; Q63HR2: TNS2; NbExp=3; IntAct=EBI-10198848, EBI-949753;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12032826,
CC ECO:0000269|PubMed:25662211}. Cytoplasm {ECO:0000269|PubMed:25662211}.
CC Note=In nuclear speckles. Relocalizes to the cytoplasm during cell
CC division. {ECO:0000269|PubMed:12032826, ECO:0000269|PubMed:25662211}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9P127-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9P127-2; Sequence=VSP_053926, VSP_053927;
CC -!- TISSUE SPECIFICITY: Expressed specifically in testis. Also expressed in
CC a wide variety of cancer types, but particularly high levels of
CC expression observed in melanoma cells. {ECO:0000269|PubMed:12032826,
CC ECO:0000269|PubMed:25662211}.
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DR EMBL; AF124430; AAF28870.1; -; mRNA.
DR EMBL; AK093369; BAG52698.1; -; mRNA.
DR EMBL; AL109751; CAC09922.1; -; Genomic_DNA.
DR EMBL; BC128134; AAI28135.1; -; mRNA.
DR CCDS; CCDS14567.1; -. [Q9P127-1]
DR RefSeq; NP_001305769.1; NM_001318840.1. [Q9P127-2]
DR RefSeq; NP_057467.1; NM_016383.4. [Q9P127-1]
DR AlphaFoldDB; Q9P127; -.
DR SMR; Q9P127; -.
DR BioGRID; 119383; 99.
DR IntAct; Q9P127; 20.
DR STRING; 9606.ENSP00000360988; -.
DR iPTMnet; Q9P127; -.
DR PhosphoSitePlus; Q9P127; -.
DR BioMuta; LUZP4; -.
DR DMDM; 74753106; -.
DR MassIVE; Q9P127; -.
DR PaxDb; Q9P127; -.
DR PeptideAtlas; Q9P127; -.
DR PRIDE; Q9P127; -.
DR ProteomicsDB; 3632; -.
DR ProteomicsDB; 83637; -. [Q9P127-1]
DR Antibodypedia; 52074; 152 antibodies from 18 providers.
DR DNASU; 51213; -.
DR Ensembl; ENST00000371920.4; ENSP00000360988.3; ENSG00000102021.11. [Q9P127-1]
DR GeneID; 51213; -.
DR KEGG; hsa:51213; -.
DR MANE-Select; ENST00000371920.4; ENSP00000360988.3; NM_016383.5; NP_057467.1.
DR UCSC; uc004eqa.4; human. [Q9P127-1]
DR CTD; 51213; -.
DR DisGeNET; 51213; -.
DR GeneCards; LUZP4; -.
DR HGNC; HGNC:24971; LUZP4.
DR HPA; ENSG00000102021; Tissue enriched (testis).
DR MIM; 300616; gene.
DR neXtProt; NX_Q9P127; -.
DR OpenTargets; ENSG00000102021; -.
DR PharmGKB; PA134920076; -.
DR VEuPathDB; HostDB:ENSG00000102021; -.
DR eggNOG; ENOG502STD9; Eukaryota.
DR GeneTree; ENSGT00950000183340; -.
DR HOGENOM; CLU_082916_0_0_1; -.
DR InParanoid; Q9P127; -.
DR OMA; GQHPLNR; -.
DR OrthoDB; 1408618at2759; -.
DR PhylomeDB; Q9P127; -.
DR TreeFam; TF350091; -.
DR PathwayCommons; Q9P127; -.
DR Reactome; R-HSA-159236; Transport of Mature mRNA derived from an Intron-Containing Transcript.
DR Reactome; R-HSA-72187; mRNA 3'-end processing.
DR Reactome; R-HSA-73856; RNA Polymerase II Transcription Termination.
DR SignaLink; Q9P127; -.
DR BioGRID-ORCS; 51213; 9 hits in 697 CRISPR screens.
DR GenomeRNAi; 51213; -.
DR Pharos; Q9P127; Tbio.
DR PRO; PR:Q9P127; -.
DR Proteomes; UP000005640; Chromosome X.
DR RNAct; Q9P127; protein.
DR Bgee; ENSG00000102021; Expressed in testis and 3 other tissues.
DR ExpressionAtlas; Q9P127; baseline and differential.
DR Genevisible; Q9P127; HS.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; mRNA transport; Nucleus; Phosphoprotein;
KW Reference proteome; RNA-binding; Transport.
FT CHAIN 1..313
FT /note="Leucine zipper protein 4"
FT /id="PRO_0000288649"
FT REGION 1..238
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1..119
FT /note="Interaction with DDX39B/UAP56"
FT /evidence="ECO:0000269|PubMed:25662211"
FT REGION 51..80
FT /note="Arg-rich; required for RNA-binding"
FT /evidence="ECO:0000303|PubMed:25662211"
FT REGION 178..236
FT /note="RS-containing His-rich (RS-H); necessary for nuclear
FT localization"
FT /evidence="ECO:0000269|PubMed:25662211"
FT REGION 238..287
FT /note="Leucine-zipper; required for RNA-binding and for its
FT relocalization to the cytoplasm during cell division"
FT /evidence="ECO:0000269|PubMed:25662211"
FT REGION 241..313
FT /note="Interaction with NXF1"
FT /evidence="ECO:0000269|PubMed:25662211"
FT REGION 290..313
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 22..40
FT /note="UAP56-binding motif (UBM); required for proper
FT nuclear localization"
FT /evidence="ECO:0000269|PubMed:25662211"
FT COMPBIAS 38..62
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 63..77
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 78..95
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 123..139
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 140..170
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 177..237
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 234
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT VAR_SEQ 1..32
FT /note="MASFRKLTLSEKVPPNHPSRKKVNFLDMSLDD -> MLKKKRIKDRTIVKRN
FT RLQDSNQKLIDIAIGE (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_053926"
FT VAR_SEQ 33..114
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_053927"
FT VARIANT 14
FT /note="P -> S (in dbSNP:rs10482480)"
FT /id="VAR_051146"
FT VARIANT 306
FT /note="T -> A (in dbSNP:rs35314601)"
FT /id="VAR_051147"
SQ SEQUENCE 313 AA; 35937 MW; E041911D9BA1DC8B CRC64;
MASFRKLTLS EKVPPNHPSR KKVNFLDMSL DDIIIYKELE GTNAEEEKNK RQNHSKKESP
SRQQSKAHRH RHRRGYSRCR SNSEEGNHDK KPSQKPSGFK SGQHPLNGQP LIEQEKCSDN
YEAQAEKNQG QSEGNQHQSE GNPDKSEESQ GQPEENHHSE RSRNHLERSL SQSDRSQGQL
KRHHPQYERS HGQYKRSHGQ SERSHGHSER SHGHSERSHG HSERSHGHSK RSRSQGDLVD
TQSDLIATQR DLIATQKDLI ATQRDLIATQ RDLIVTQRDL VATERDLINQ SGRSHGQSER
HQRYSTGKNT ITT
