LVGA_LEGPH
ID LVGA_LEGPH Reviewed; 208 AA.
AC Q5ZY48;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 03-AUG-2022, entry version 65.
DE RecName: Full=Type 4 adapter protein LvgA {ECO:0000305};
GN Name=lvgA {ECO:0000303|PubMed:16803597};
GN OrderedLocusNames=lpg0525 {ECO:0000312|EMBL:AAU26621.1};
OS Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC
OS 33152 / DSM 7513).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales;
OC Legionellaceae; Legionella.
OX NCBI_TaxID=272624;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Philadelphia 1 / ATCC 33152 / DSM 7513;
RX PubMed=15448271; DOI=10.1126/science.1099776;
RA Chien M., Morozova I., Shi S., Sheng H., Chen J., Gomez S.M., Asamani G.,
RA Hill K., Nuara J., Feder M., Rineer J., Greenberg J.J., Steshenko V.,
RA Park S.H., Zhao B., Teplitskaya E., Edwards J.R., Pampou S., Georghiou A.,
RA Chou I.-C., Iannuccilli W., Ulz M.E., Kim D.H., Geringer-Sameth A.,
RA Goldsberry C., Morozov P., Fischer S.G., Segal G., Qu X., Rzhetsky A.,
RA Zhang P., Cayanis E., De Jong P.J., Ju J., Kalachikov S., Shuman H.A.,
RA Russo J.J.;
RT "The genomic sequence of the accidental pathogen Legionella pneumophila.";
RL Science 305:1966-1968(2004).
RN [2]
RP FUNCTION, INTERACTION WITH ICMS, SUBCELLULAR LOCATION, INDUCTION, AND
RP DISRUPTION PHENOTYPE.
RC STRAIN=Philadelphia 1 / Lp02;
RX PubMed=16803597; DOI=10.1111/j.1365-2958.2006.05243.x;
RA Vincent C.D., Vogel J.P.;
RT "The Legionella pneumophila IcmS-LvgA protein complex is important for
RT Dot/Icm-dependent intracellular growth.";
RL Mol. Microbiol. 61:596-613(2006).
RN [3]
RP FUNCTION, AND SUBUNIT.
RC STRAIN=Philadelphia 1 / Lp01;
RX PubMed=32513920; DOI=10.1038/s41467-020-16681-z;
RA Meir A., Mace K., Lukoyanova N., Chetrit D., Hospenthal M.K., Redzej A.,
RA Roy C., Waksman G.;
RT "Mechanism of effector capture and delivery by the type IV secretion system
RT from Legionella pneumophila.";
RL Nat. Commun. 11:2864-2864(2020).
RN [4] {ECO:0007744|PDB:5X90}
RP X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS) OF 22-195 IN COMPLEX WITH DOTL; ICMS
RP AND ICMW, FUNCTION, AND SUBUNIT.
RC STRAIN=Philadelphia 1 / ATCC 33152 / DSM 7513;
RX PubMed=28714967; DOI=10.1038/nmicrobiol.2017.114;
RA Kwak M.J., Kim J.D., Kim H., Kim C., Bowman J.W., Kim S., Joo K., Lee J.,
RA Jin K.S., Kim Y.G., Lee N.K., Jung J.U., Oh B.H.;
RT "Architecture of the type IV coupling protein complex of Legionella
RT pneumophila.";
RL Nat. Microbiol. 2:17114-17114(2017).
RN [5] {ECO:0007744|PDB:7BWK}
RP X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS) IN COMPLEX WITH DOTL; ICMS; ICMW AND
RP VPDB, FUNCTION, SUBUNIT, AND MUTAGENESIS OF ILE-153.
RX PubMed=32457311; DOI=10.1038/s41467-020-16397-0;
RA Kim H., Kubori T., Yamazaki K., Kwak M.J., Park S.Y., Nagai H., Vogel J.P.,
RA Oh B.H.;
RT "Structural basis for effector protein recognition by the Dot/Icm Type IVB
RT coupling protein complex.";
RL Nat. Commun. 11:2623-2623(2020).
CC -!- FUNCTION: Component of the Dot/Icm type IVB secretion system (T4BSS),
CC which is used to inject bacterial effector proteins into eukaryotic
CC host cells (PubMed:32513920, PubMed:28714967, PubMed:32457311). Part of
CC a subcomplex which recruits effector proteins and delivers them to the
CC core transmembrane subcomplex (PubMed:32513920, PubMed:32457311). Is a
CC critical subunit for binding a subset of effector proteins
CC (PubMed:28714967, PubMed:32457311). Recognizes more than one type of
CC binding motif (PubMed:32457311). May be a critical factor that confers
CC host specificity (PubMed:16803597). {ECO:0000269|PubMed:16803597,
CC ECO:0000269|PubMed:28714967, ECO:0000269|PubMed:32457311,
CC ECO:0000269|PubMed:32513920}.
CC -!- SUBUNIT: The T4BSS is a complex nanomachine composed of several
CC subcomplexes. This subunit is part of the Type IV Coupling Complex
CC (T4CC), a subcomplex composed of the DotLMNYZ core and the IcmSW-LvgA
CC adapter subunits, linked by the C-terminal tail of DotL
CC (PubMed:28714967, PubMed:32457311, PubMed:32513920). Interacts with
CC DotL, IcmS and IcmW (PubMed:28714967, PubMed:16803597). Interacts with
CC various effector proteins, including VpdB, SetA, PieA and SidH
CC (PubMed:32457311). {ECO:0000269|PubMed:16803597,
CC ECO:0000269|PubMed:28714967, ECO:0000269|PubMed:32457311,
CC ECO:0000269|PubMed:32513920}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16803597}.
CC -!- INDUCTION: Constitutively expressed in vitro.
CC {ECO:0000269|PubMed:16803597}.
CC -!- DISRUPTION PHENOTYPE: Mutant is differentially attenuated in diverse
CC hosts (PubMed:16803597). Inactivation of the gene causes a minimal
CC defect in growth in the human monocytic cell line U937 and the
CC environmental host A.castellanii, but mutant is severely attenuated for
CC intracellular growth in mouse macrophages (PubMed:16803597). Mutant
CC appears to assemble a fully functional Dot/Icm complex and exhibits
CC wild-type levels of contact-dependent cytotoxicity (PubMed:16803597).
CC {ECO:0000269|PubMed:16803597}.
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DR EMBL; AE017354; AAU26621.1; -; Genomic_DNA.
DR RefSeq; WP_010946272.1; NC_002942.5.
DR RefSeq; YP_094568.1; NC_002942.5.
DR PDB; 5X90; X-ray; 2.80 A; D=25-195, H=22-193.
DR PDB; 7BWK; X-ray; 2.80 A; D/I=1-208.
DR PDBsum; 5X90; -.
DR PDBsum; 7BWK; -.
DR SMR; Q5ZY48; -.
DR STRING; 272624.lpg0525; -.
DR PaxDb; Q5ZY48; -.
DR PRIDE; Q5ZY48; -.
DR EnsemblBacteria; AAU26621; AAU26621; lpg0525.
DR GeneID; 66489720; -.
DR KEGG; lpn:lpg0525; -.
DR PATRIC; fig|272624.6.peg.549; -.
DR eggNOG; ENOG5031DQG; Bacteria.
DR HOGENOM; CLU_1314124_0_0_6; -.
DR OMA; DHEFVYS; -.
DR Proteomes; UP000000609; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Protein transport; Reference proteome; Transport;
KW Virulence.
FT CHAIN 1..208
FT /note="Type 4 adapter protein LvgA"
FT /id="PRO_0000455597"
FT MUTAGEN 153
FT /note="I->E: Cannot bind the effector protein VpdB.
FT Decreases binding affinity for SetA, PieA and SidH."
FT /evidence="ECO:0000269|PubMed:32457311"
SQ SEQUENCE 208 AA; 23506 MW; 44FADEA633D6E46F CRC64;
MADGDIEIKA GFVDTDLDDR KLTMIDDLNN PLAIVERVYL IWWHWADFHL HVISPHIDTI
TPAIVIEPEL IPGSNDHEFV YSIHDSGSKL STSKSQDMFS AGMSMCKLFY TIEKMVYILV
ERLKSGGVSM EAEVQIAFAG HEIAQRKAFE SIINLPYNVV VTNFDPGIWG EKYLQNVKRL
ADKGYGYPPE SPRKIYMHPV SSGTTARK
