LVSA_DICDI
ID LVSA_DICDI Reviewed; 3619 AA.
AC Q55DM1; Q9U573;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 02-MAY-2006, sequence version 2.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=BEACH domain-containing protein lvsA;
DE AltName: Full=Large volume sphere mutant protein A;
GN Name=lvsA; ORFNames=DDB_G0269150;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=AX3 / DH1;
RX PubMed=10588668; DOI=10.1091/mbc.10.12.4429;
RA Kwak E., Gerald N., Larochelle D.A., Vithalani K.K., Niswonger M.L.,
RA Maready M., De Lozanne A.;
RT "LvsA, a protein related to the mouse beige protein, is required for
RT cytokinesis in Dictyostelium.";
RL Mol. Biol. Cell 10:4429-4439(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP FUNCTION.
RX PubMed=11865029; DOI=10.1242/jcs.115.4.737;
RA Cornillon S., Dubois A., Bruckert F., Lefkir Y., Marchetti A.,
RA Benghezal M., De Lozanne A., Letourneur F., Cosson P.;
RT "Two members of the beige/CHS (BEACH) family are involved at different
RT stages in the organization of the endocytic pathway in Dictyostelium.";
RL J. Cell Sci. 115:737-744(2002).
RN [4]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=11872142; DOI=10.1034/j.1600-0854.2002.30107.x;
RA Gerald N.J., Siano M., De Lozanne A.;
RT "The Dictyostelium LvsA protein is localized on the contractile vacuole and
RT is required for osmoregulation.";
RL Traffic 3:50-60(2002).
RN [5]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=15086784; DOI=10.1111/j.1600-0854.2004.00177.x;
RA Wu W.-I., Yajnik J., Siano M., De Lozanne A.;
RT "Structure-function analysis of the BEACH protein LvsA.";
RL Traffic 5:346-355(2004).
CC -!- FUNCTION: Involved in myosin-independent cytokinesis and early steps of
CC phagocytosis. Also involved in contractile vacuole-mediated
CC osmoregulation. {ECO:0000269|PubMed:10588668,
CC ECO:0000269|PubMed:11865029, ECO:0000269|PubMed:11872142,
CC ECO:0000269|PubMed:15086784}.
CC -!- SUBCELLULAR LOCATION: Contractile vacuole membrane
CC {ECO:0000269|PubMed:11872142, ECO:0000269|PubMed:15086784}; Peripheral
CC membrane protein {ECO:0000269|PubMed:11872142,
CC ECO:0000269|PubMed:15086784}.
CC -!- DOMAIN: Deletion of N-terminal region (689 aa) causes partial loss of
CC function, while deletions of the first 1828 residues resembles the null
CC mutant.
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DR EMBL; AF088979; AAD52096.2; -; Genomic_DNA.
DR EMBL; AAFI02000005; EAL71925.2; -; Genomic_DNA.
DR RefSeq; XP_646110.2; XM_641018.2.
DR SMR; Q55DM1; -.
DR STRING; 44689.DDB0191124; -.
DR PaxDb; Q55DM1; -.
DR PRIDE; Q55DM1; -.
DR EnsemblProtists; EAL71925; EAL71925; DDB_G0269150.
DR GeneID; 8617060; -.
DR KEGG; ddi:DDB_G0269150; -.
DR dictyBase; DDB_G0269150; lvsA.
DR eggNOG; KOG1786; Eukaryota.
DR eggNOG; KOG1788; Eukaryota.
DR HOGENOM; CLU_000175_5_0_1; -.
DR InParanoid; Q55DM1; -.
DR OMA; GVCHLIE; -.
DR PhylomeDB; Q55DM1; -.
DR PRO; PR:Q55DM1; -.
DR Proteomes; UP000002195; Chromosome 1.
DR GO; GO:0031164; C:contractile vacuolar membrane; IDA:dictyBase.
DR GO; GO:0000331; C:contractile vacuole; IDA:dictyBase.
DR GO; GO:0005737; C:cytoplasm; IDA:dictyBase.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0008289; F:lipid binding; IDA:dictyBase.
DR GO; GO:0019901; F:protein kinase binding; IBA:GO_Central.
DR GO; GO:0044877; F:protein-containing complex binding; IDA:dictyBase.
DR GO; GO:0000915; P:actomyosin contractile ring assembly; IMP:dictyBase.
DR GO; GO:0032060; P:bleb assembly; IMP:dictyBase.
DR GO; GO:0007155; P:cell adhesion; IMP:dictyBase.
DR GO; GO:0006874; P:cellular calcium ion homeostasis; IMP:dictyBase.
DR GO; GO:0140026; P:contractile vacuole dissociation from plasma membrane; IMP:dictyBase.
DR GO; GO:0033298; P:contractile vacuole organization; IMP:dictyBase.
DR GO; GO:0007032; P:endosome organization; IMP:dictyBase.
DR GO; GO:0000281; P:mitotic cytokinesis; IMP:dictyBase.
DR GO; GO:0006909; P:phagocytosis; IMP:dictyBase.
DR GO; GO:0006907; P:pinocytosis; IMP:dictyBase.
DR GO; GO:0008104; P:protein localization; IBA:GO_Central.
DR CDD; cd06071; Beach; 1.
DR CDD; cd01201; PH_BEACH; 1.
DR Gene3D; 1.10.1540.10; -; 1.
DR Gene3D; 2.130.10.10; -; 2.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR000409; BEACH_dom.
DR InterPro; IPR036372; BEACH_dom_sf.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR031570; NBEA/BDCP_DUF4704.
DR InterPro; IPR023362; PH-BEACH_dom.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF02138; Beach; 1.
DR Pfam; PF15787; DUF4704; 1.
DR Pfam; PF14844; PH_BEACH; 1.
DR Pfam; PF00400; WD40; 2.
DR SMART; SM01026; Beach; 1.
DR SMART; SM00320; WD40; 5.
DR SUPFAM; SSF49899; SSF49899; 1.
DR SUPFAM; SSF50978; SSF50978; 1.
DR SUPFAM; SSF81837; SSF81837; 1.
DR PROSITE; PS50197; BEACH; 1.
DR PROSITE; PS51783; PH_BEACH; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 4: Predicted;
KW Coiled coil; Membrane; Phagocytosis; Reference proteome; Repeat; Vacuole;
KW WD repeat.
FT CHAIN 1..3619
FT /note="BEACH domain-containing protein lvsA"
FT /id="PRO_0000327707"
FT REPEAT 94..133
FT /note="WD 1"
FT REPEAT 2294..2335
FT /note="WD 2"
FT DOMAIN 2807..2932
FT /note="BEACH-type PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01119"
FT DOMAIN 2972..3270
FT /note="BEACH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00026"
FT REPEAT 3347..3386
FT /note="WD 3"
FT REPEAT 3389..3428
FT /note="WD 4"
FT REPEAT 3431..3471
FT /note="WD 5"
FT REPEAT 3474..3518
FT /note="WD 6"
FT REPEAT 3563..3602
FT /note="WD 7"
FT REGION 1..117
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 648..709
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1101..1129
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1367..1390
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1636..1658
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1893..1924
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1964..1999
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2403..2457
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2596..2785
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2940..2971
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3516..3539
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 2234..2258
FT /evidence="ECO:0000255"
FT COMPBIAS 31..117
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 656..698
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1367..1389
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1636..1654
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2940..2954
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 358
FT /note="I -> V (in Ref. 1; AAD52096)"
FT /evidence="ECO:0000305"
FT CONFLICT 1358
FT /note="N -> D (in Ref. 1; AAD52096)"
FT /evidence="ECO:0000305"
FT CONFLICT 1362
FT /note="S -> N (in Ref. 1; AAD52096)"
FT /evidence="ECO:0000305"
FT CONFLICT 2553
FT /note="P -> Q (in Ref. 1; AAD52096)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 3619 AA; 408030 MW; C71BC8A29CC19C49 CRC64;
MFRRFKDLIG TSEDPTPQVP HSPGHPPHQP PQQQQQQQQQ QQQQQQQQQQ QQQQQQQPQP
QQPLSPRSVS SPIGSTTSSN STSSFSSPVR KYSSATEESS SINSNNNNNN NKNNNNNNNS
NIIESNINVW TIMIGSEVEV IRSQATAQKL KLLWQEFLSS KSDKDKVMRL NKLLPYFIGL
YEDKKIDTKS PMVDIFGNNS KSFSFAISRR LVKDINEIMK QANSPQQPPQ PPQLIKETIA
KEIYKFFSTT SGQVSGFELL YSIEILSESN TSCAEAMAEA SIPSMLVRCL QYFFLVPYTT
MMETTKGIIE EKLIRTLCFL SKQKSAIEEL QKTDTLSTLF ALMSNECPPS HRPLRAKIGS
FGLELTDLYP PTITYINSKR VIANIIKDLT NYYMFTPESY VTLCRIIIKI LSESSKKSTI
LLDEFQRNDG YTFLVDSLFR LESSKDKPAL FEQLLDSICS LVYIGYGNVS LPVENSSVPY
QTSINNIKEI SNQNYISKNG NAFKVLERYF LKSNYEENRV KILDRILSVY SSNTVNFILL
QHTSTLTKFI QEYESLSNGL KYHVMKIVCF VVTVLNCVPF QELSTFSLLV GENPSFYTLE
MINQLITTLV NFEFRYKHIF RETGLLDILV KVIDVIAQDI IRLNNSKKID DDDENNNNNN
NNNNNNNNNN NNNNDNDNNN NNDNNNEENG SGSNGPIVPC MTGNGEKEAD SNDQALNSII
KVESFQILLD SLFILISENP DNISLIRSFS IFNILLRFLP YSSVRGKSLR ILQQLIKYDP
EPTQKEFDGL IKVLTSVNKE NYPMKSDILN ATRKLFNISK HARDSFREHG GFVSIISVFI
SLESSFSPNR KDSRNWDMEK LELIESICRC TTSALCGNVI NRENFEQQIG YKTFSSCLIM
TGVLGTEFSK SVVDFIFDMV TENLNASDQI SNQMIINNVE SFNVILDIIP HIENKDFRLQ
IISRINKMAE YGRYNQEALS KLSIPDWILS RFPSNLSNAN DPLQPLLLSL IQTVGANCLS
GSELRQFVKL LQPEHSPEVL LKILSSMAKS PPTPPYFEFN LSKIPFGYIR VPITERAWPP
TNGYTIMFWL YIDKFPTVNN NNNNNNSSNN SNNSNNSNNN NNNNNNNDQI DLVHIYSDDK
KSSLYIYLKN GIITVNIINS SKYVIEIPSY KFVEGKWYHI GIVHARRLLG GTDFKLFVDG
FLKYTATKAQ YPAQITSGSM LICDIGVSNQ NRFPTDSIWR IGTFYLLEDS LGAKHINTIY
FLGPNYASNF KGRFSPYQTY EIVNSANLMA IKDLDYGDQL GPLNLAKVSM QIDENKILVG
LCASNKLIRT NNSSKVVYNE IFNGIINELS QNHGVALNVF SSPNGTSPNL TGLQNNNNNN
NNSGGSNSKK DLEGRVEIIN QADLTTKLRG VLIGSVEAFR RNKVADSIKK IGGMPISLLL
LEKANSEETL FDSLGLLVGL IQYHPTNTHE MSQINGYELL AWVLKKKASL GLFNSNILEL
LFDLIGINGN CSTTITSRAP QEGTVANWNA CKYIMMNWDI WRLTTPALQR HVINGYNSLI
VNNIQRRFNI DSLRKVNVIQ EIFDILSSST NEEPLPESVA SSVINVLYNI LSYGGLIEDD
IRQISAFLIS HLHKDIPTPS SSSSSSSTSS TSSRRKSIHR SKLATMELSN TATIQLVNHV
FYTFLKVVSN CQTQETAAIF RRVSSYWCFF FIDENLPPLT VSLALRVTCI FFLYKYDYCS
TFIKKSGFKL LEKVLPSLSG HQEIYLCLLH LLLGGDPKLL VDLDLSSSSG GAGGTTIVFH
ELLRIFTPFE KSLYCIEAAQ LILSLIKRSY EDNYQYLEQK QQELQNANQG LDNDELLSSL
ISNVNINNSI NNNDNSNSPL STFQTISRSV SSSSISSNIS SSSSSSTLVN SSNSNNNNNT
PTSGLASTIT KFGNLWNKFE EKTLEFAVTT GAIDENSTGA TDAQQAALKK KNRMSIQSSP
FQSKNLGTGG DDSVTNTPNG SSLHNRVTGM DDDSKLNGAL GGGGGGGGGG VSVGDNQPIN
FNLYDEMLPE LKISQFATPE ESSNLQYTML TYFIYLFHEN QYFQQECYSQ PMVEELISIL
FPNGKINLPP LYNSTGQTNG IKDRVLDLVV KFLCQIMLSA MRKTSKAISI IEMVLEGAPT
TATDEEFILY HSRILLDLMY VVETNITKTE FFDNERVHSN LIKLSSMLVD RVNLDQLVKN
NKIIIAKRIF LFIVKILEKL EADRVGLQKT VQSLYKSLNR IILYLINHTT DTDLSFVANH
IINHQRIIFS ENNLDSDFMN AFCYPLYKLV ISDQHEHVDN SIKLWRLLLS LKTSSYIESL
ATVLQLKVSS GSNQRQSEII DLKPGFELLR NTSGNGAFNN DEFKLWINDN IQTITQVFEE
NPKKQHLSFK NNEKKHSSEH TLPSLKSRRT ERLSKKQRQD RKDQSHQEEK SKHITKKAQY
FVRSESDRRK KIKQLESDKQ KFNAIQWENM RAQITRERAV WGPSEPHPLD KWKLDSTEGP
YRMRKKMEKN YNFYKNYPYV PPSFDEQNNS LLPIPCSADS ETYMNIVGTE EANLLESSYW
KFDLLSTNQV ITSSTNTSSI TNNNNNNNNN NNNNNNNNNN TITKSTSQNA NNNNNANNMN
QSTSSSSSNT TTTTTPQQSS SQIKVSSPEL SSNEITPPTS PVQSSSEDVF KSPKLQSSTV
EGQLSRNPSS SELFNDNSST ISEENSSLTS ASTTLSPPPP STQTTTTTTT STPTTQSSVA
TTTTGNTNEV DEETSTNNQT TSEDETQAFI RLLDPYDQSY LKDAMRKDPR LNGIMYNCGS
VDGMDKIEGI LIFCPVYMYI FDGYYKDENT GDISEVEEKI NSEWLPEGTV LPMKKKIIHY
FLKWAYEDIR DVLKRRYLLR QVALEIFSTD GRNNLVVYRD EPTRDEVYHT LVNNVSSHNT
IGGDAQGITG GQTGNDDNDD HHGGGGGRGV RDRFTSIWRK SPLTLKWQQG QISNFQYLMH
LNTLAGRSYN DLTQYPVFPW VLSDYESEEL DIDDPKVYRD LSKPMGALEE SRAQKFRERF
ENWDDQEPNE HGHKVPKFHY GTHYSSAAIV LYYLIRLEPF TQHFLKLQGG RWDQPDRLFS
SITEAWASSS QGSTGVVMEL IPEFYYLDEF LVNNNKFNFG TKQGGEPIDD IILPPWAKGS
PQEFIKLHRK ALESDYVSEH LHEWIDLIFG YRQQGKAADD SLNVFYYLTY EGAVNIDAIS
DPVEKAATIA QINNFGQTPK QLFDKPHPKR NATLMGLPFY AKALTGNFIK DIGEPVGQIR
LINDRATCVG FNKVLLPPNH SKYMLWGLPD GSIRYNTGDK IKVLEDHHDG PLTCLTATED
GRICVSGGSD SLICVYNLKR FSLAKRLSGH TGSITCVSAS RPYSIIVSGS DDRTCIIWDL
NRLCYVRSLD AHEGPISCIG IHDTTGEIVV CSGTTISVYT VNGELLINYK TSQIANDQIT
CCIWSKGPEW LGENVLLTGH RDGKVKVWGL ETRLLPDNNN SNNNNNNNNN NNNNATQIPS
TNNNKLKFKN VIILRATFSN SQSHSTAITS IFLTNDQQKF YTGDITGRVC MWSDNEASQV
KQRGWINTDI RGILQSEKK
