LVSB_DICDI
ID LVSB_DICDI Reviewed; 4118 AA.
AC Q86JF2; Q55AT1; Q8IHL0;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 23-FEB-2022, entry version 110.
DE RecName: Full=BEACH domain-containing protein lvsB;
GN Name=lvsB; ORFNames=DDB_G0271504;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 416-4118, AND FUNCTION.
RC STRAIN=NC4A2;
RX PubMed=12210762; DOI=10.1002/jcb.10254;
RA Wang N., Wu W.I., De Lozanne A.;
RT "BEACH family of proteins: phylogenetic and functional analysis of six
RT Dictyostelium BEACH proteins.";
RL J. Cell. Biochem. 86:561-570(2002).
RN [4]
RP FUNCTION.
RX PubMed=11854420; DOI=10.1091/mbc.01-09-0454;
RA Harris E., Wang N., Wu Wl W.L., Weatherford A., De Lozanne A., Cardelli J.;
RT "Dictyostelium LvsB mutants model the lysosomal defects associated with
RT Chediak-Higashi syndrome.";
RL Mol. Biol. Cell 13:656-669(2002).
RN [5]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=17488289; DOI=10.1111/j.1600-0854.2007.00567.x;
RA Kypri E., Schmauch C., Maniak M., De Lozanne A.;
RT "The BEACH protein LvsB is localized on lysosomes and postlysosomes and
RT limits their fusion with early endosomes.";
RL Traffic 8:774-783(2007).
RN [6]
RP FUNCTION.
RX PubMed=17606989; DOI=10.1242/jcs.009001;
RA Charette S.J., Cosson P.;
RT "A LYST/beige homolog is involved in biogenesis of Dictyostelium secretory
RT lysosomes.";
RL J. Cell Sci. 120:2338-2343(2007).
CC -!- FUNCTION: Involved in negative regulation of lysosome biogenesis, by
CC limiting the heterotypic fusion of early endosomes and postlysosomal
CC compartments. {ECO:0000269|PubMed:11854420,
CC ECO:0000269|PubMed:12210762, ECO:0000269|PubMed:17488289,
CC ECO:0000269|PubMed:17606989}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}. Lysosome {ECO:0000269|PubMed:17488289}. Endosome
CC {ECO:0000269|PubMed:17488289}. Note=Also found in post-lysosome.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAN38985.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AAFI02000006; EAL71616.2; -; Genomic_DNA.
DR EMBL; AY159038; AAN38985.1; ALT_SEQ; Genomic_DNA.
DR RefSeq; XP_645615.2; XM_640523.2.
DR SMR; Q86JF2; -.
DR STRING; 44689.DDB0185107; -.
DR PaxDb; Q86JF2; -.
DR PRIDE; Q86JF2; -.
DR EnsemblProtists; EAL71616; EAL71616; DDB_G0271504.
DR GeneID; 8618070; -.
DR KEGG; ddi:DDB_G0271504; -.
DR dictyBase; DDB_G0271504; lvsB.
DR eggNOG; KOG1786; Eukaryota.
DR HOGENOM; CLU_223969_0_0_1; -.
DR InParanoid; Q86JF2; -.
DR OMA; MSITKKW; -.
DR PRO; PR:Q86JF2; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005768; C:endosome; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005764; C:lysosome; IDA:dictyBase.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0045335; C:phagocytic vesicle; IDA:dictyBase.
DR GO; GO:0019901; F:protein kinase binding; IBA:GO_Central.
DR GO; GO:0009267; P:cellular response to starvation; IMP:dictyBase.
DR GO; GO:0031154; P:culmination involved in sorocarp development; HMP:dictyBase.
DR GO; GO:0006887; P:exocytosis; IMP:dictyBase.
DR GO; GO:0007041; P:lysosomal transport; IMP:dictyBase.
DR GO; GO:0007040; P:lysosome organization; IMP:dictyBase.
DR GO; GO:1905362; P:negative regulation of endosomal vesicle fusion; IMP:dictyBase.
DR GO; GO:0001845; P:phagolysosome assembly; IMP:dictyBase.
DR GO; GO:0008104; P:protein localization; IBA:GO_Central.
DR GO; GO:0006970; P:response to osmotic stress; IMP:dictyBase.
DR CDD; cd06071; Beach; 1.
DR Gene3D; 1.10.1540.10; -; 1.
DR Gene3D; 1.25.10.10; -; 1.
DR Gene3D; 2.130.10.10; -; 2.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000409; BEACH_dom.
DR InterPro; IPR036372; BEACH_dom_sf.
DR InterPro; IPR023362; PH-BEACH_dom.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF02138; Beach; 1.
DR Pfam; PF14844; PH_BEACH; 1.
DR Pfam; PF00400; WD40; 1.
DR SMART; SM01026; Beach; 1.
DR SMART; SM00320; WD40; 5.
DR SUPFAM; SSF48371; SSF48371; 1.
DR SUPFAM; SSF50978; SSF50978; 1.
DR SUPFAM; SSF81837; SSF81837; 1.
DR PROSITE; PS50197; BEACH; 1.
DR PROSITE; PS51783; PH_BEACH; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 4: Predicted;
KW Coiled coil; Endosome; Lysosome; Membrane; Reference proteome; Repeat;
KW Transmembrane; Transmembrane helix; WD repeat.
FT CHAIN 1..4118
FT /note="BEACH domain-containing protein lvsB"
FT /id="PRO_0000327708"
FT TRANSMEM 198..218
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 827..847
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 3303..3479
FT /note="BEACH-type PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01119"
FT DOMAIN 3491..3782
FT /note="BEACH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00026"
FT REPEAT 3868..3907
FT /note="WD 1"
FT REPEAT 3924..3963
FT /note="WD 2"
FT REPEAT 3984..4027
FT /note="WD 3"
FT REPEAT 4029..4073
FT /note="WD 4"
FT REPEAT 4075..4114
FT /note="WD 5"
FT REGION 1..35
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 236..267
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 332..382
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 621..644
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1155..1213
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1599..1622
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1643..1681
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1928..1968
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2015..2044
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2537..2574
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2702..2741
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2754..2791
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2902..3007
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3245..3265
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3348..3418
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 2705..2738
FT /evidence="ECO:0000255"
FT COMPBIAS 236..258
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1155..1189
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1190..1204
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2537..2573
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2704..2737
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3348..3417
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 2030
FT /note="N -> T (in Ref. 3; AAN38985)"
FT /evidence="ECO:0000305"
FT CONFLICT 4104
FT /note="L -> W (in Ref. 3; AAN38985)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 4118 AA; 463997 MW; E87919A55B1B0EBC CRC64;
MNRNFNNINN NNNNNNNYHG YQYHQQQQQQ NQQQQQQQYN NNVIKYLWNT YTQTVVYQDS
LFNLVEFLQF FTLNYYNDTI GDLLFLSNGT LTDITRHLSK HLIEEIGYSC TLLNQQQQTQ
QTQTQTQSKQ QTIPQLSLNN SIPTSFTTTA SSTNTTPNAG LKLAEYEIYQ YLIGYSTAGE
GPLVLAALDI LSRQSPNGIP LSFLNFLITI LLRILSLPPE LLYNNNNNDN NNNINNFSGN
NNNNFNNNNH YFNNNHNNHN HHYQHHQLKE KKKNVNKQHF KPIESFFYLQ PLPSSLSDMF
RDDDYASLSS SYGGGGNLTP LSLSSNHAFS PPLSSLKNSS SNTFTKPSSS TTTTTTTTTQ
TPTPNSNTDS KQNKDNNNSK NNNKEVSSVV LLGYQILKIV ENLVKDKLFL YELVTSDCFS
QLLNIFYKLP ILSTTASSLP PNSSFSIIYQ QLSQIFAQIM SKSCLSSDTI VLIHNFKLIK
NLLNIMDKLK EKDEYNMNLI IELNKIILFS IKSSTSITKL LHDDFTKANG YSILLNSMIF
ISDYGTLKNK IDYIQTTTNL LFIGHRSSYL DKANTKMFEI YLNVFSISTS EETKTELLKC
IKGVFYTSLI EESSSSSSLI ISSSDNNNNN NNNSDGVNDD KNNNNNNRDN IIFEDVIDMD
GNDSYLIFHQ FQPFTILFSQ FDTLNINNRR MILDMMDVLL TKNRISLNEL KEYCNLFKSE
LPSTVLLVSQ HLTELLTKGK VSCDVLGRDL SLTQKLLEFL VNDPTTLRFS SILLQNETEL
QLCFSILSTN NNNNNSSSKY YSNNIQQQQQ QQQQQQQQQK KRHVSRYLVL YMIVTEILSL
LLELLVPPSI QTIFIQECQG LKLLYPMLLD EYLIQPALKV IASIAIGGLQ LESRIIPDLI
MELQNNGGGA TNLDSKVLTM RKNIFSSMCY IFHNNPNSKN SFRNFQGFTW SLSILDGISR
YLISIDNNNN NSSNNNSNDN LNDFKESLDH SCNGVLNSNN SSNNSNLNGG IGNEIKKSSF
NEIFYFLKVL IDMLSAAMKN NSINQEYFRK EIIIISRSLK QCKYMEGIHA ISLCDSLLNM
AITGSWPPSC ENHSLEEGSL FSLYSPITSF FPINTTLSKN FDISFILKFE KKEQMEQQLK
QQILLNSNNN INNGNGGSIS PSSIINNMNS PGGGSSSSND SLKSPTRRSR SYTNDFHLDK
SSRIPHPPDF NNNNDTMNYD YNFQDDYIES LNSKSSSSFS LDLASVTGDS EIVYYGGSTT
SRMIHSPGLP SSSLQALSSN STKLPEKSSL QLAQERYICC QSCRDTLTLE MPEIFKLIIE
LMSSDDDISQ TEIKSSCYII RELIFLTNCS LANQKRLSSL LIDIITHFKP LLLSIILYQK
QQQQQQQQQQ QSNSNSNSNS TIILKKKINN ETSTKLKPLL LDLIQTLAGH NLTLIEFRKY
FELLKVKDSN NQFIYPIDLL NLLLKISSNR DNIPLYYAEL SKHGLEYIDF PSWGERTWPP
TKGFGISFWF RYSLPCLNIN KSPIYLLSIE GSIGNSSTKS CECQLILENG KLVYKIYHFS
GICETYHFSD HKFEPDQFYH ISISHSSLLS TTTTTATTAN TTTNSTTAST TTTTTNSTTA
VSSTTNSIST TAAVAAITTS DGNSGISREN SLGGRDSIGS NSSSSSSSSN SGVSSGSSTN
LNKKSPVKLY VNGCLRGQIL ANYPKSNSMT LYIRFGGVNA ASSVISNNQD VQSFNVNNSW
NLGNAYFFEE VPLDKEIFYL YLLGPNHFRG LKVDLSAVDS IQPSLDKVSN LHPFLIDHLL
NPTVQPLLSL HEKIMYIFTA KCLYVTTHRI TNKTESVTHA SALPQLSTIL FQQQQLQQQQ
LQQQITNSTT SILNELNLSN SINLIRRGSE NSLITSSSSS SLSLSLSSNT NNNNNNNTRS
SLERSISGNF LQSPSSSNNN LRERSINNNN NNNNNSNNNN NNNSISSSTS SLSSSVISLV
AGAMAVPSPP SPNKLGSGIN GSSILNASLT TLSISTNNNS NNSNNSNSNN NNNNNNTNYN
SNSLNNSLNH QALSLLHTGL PIALPRGVIS QIGVKDAIMN SGGVSVIIYL IAMAEDKEYQ
RSGLKLLQSI IHNSQLNLKD MKEISGYQLV SFLIRKKNWV LDDQLLSILF SFVGIQSTRT
SIHYVDGVVQ DVLALKHFLL ERSIWRRASL IDQKKLFESL EKLVNVLHEN HEFNIIKFRQ
AGAYETILKM CREDDLPLEL LSTLTKILRS ITTNKSNKLK DDLQLILSWL LETMPKQQIF
KKYNSFNGLN STNNSNNNNN NPISLFRKGR KTLLQVQFDN HQLYLQQQQQ QNLIPQVNSN
NNNEESNNGI EGIIRINILN LLLDILSKTE LSVVEDFHSI CSLETIFGLL TCESMVSRVM
FLKIIDIFLH SQMIFGHFQK MKGFHLLGHQ LLPFETSEKI FGVLFCILFG KPSNSDILEG
LSMRMYFLSH LSDAEMKYPG AIVTILIILC NSNSTTQHNV IKMIHNIFLQ NDQFKQSLLD
NELIPRLVDI LSSNYQRRGS SSSSTNSTTN NNNNNSSTTT TSNNNNNNNE NNNEDDWVAE
ESILSLLKEI ALYGAKSQDG SAALLRDILV VLHLNTRMDY DYICCLQRRV LFDVISFFND
NIHSFSSIDS LVSSFEKLCV LTIHTLSYQE KLLLTNGTGT GGGTGGSGGG LLTKKSFRSN
LFSPSRSKEK EKEKEKEKEK EKEKEKERER ERETTNVTSD SDNILSANDI FYGDQSNEES
STTDSDSTSD NNNNNNRNSY SGRNIRGNGI NNNNNKNKFI PIWIKEGNLL DQEEFIRSLL
KVLSKSKIPQ SNTTYRNLFS TQYSARSLLC KFIFILLTFD EFKDYYLMVL QELSNMVPSL
SNTPISSSPI ISPINNNNNY YNTNSNTNSN SSTPSLFSIT SNNNNNNNNN NNNNNNNNNN
NNTNSTNQTI TDTTLSPASS NVSISNQSTP ISNNNNNNNS SGGGSGGSNI NIPPTINISD
SNSNSNEQQG ISSVLSEIIM EEDFILVLLH ITHKFLQSGN DYEIQRNSFR LWVSIIQHCS
QVDYVKKAFD TTTTSLAVLS SSQEIRDLLI RYQQIYVENE FKKWEEKFNQ SKKEWKLQYF
ETQKNKQSMI TKNQDITKST KKLSDSLIQL KSEYEKSNYQ YLIENRESKR FFQSQWKLLI
KKVTHEKAIW SPNYEISNSS STNITTTTTT TTTAKKWKLD PTEGLNRMRM RLKVISDNTS
NSHIPLIPDL STPSTPPSPQ NTKDQLLFNF NNYNNNNNNN NNNGLNAPPL HGSLDYSSFD
NLKLGEKVNE VFKCSCISPF YQRDGELLIG DQNVYFLDEL LTSADRKKTT ASTVGGSNNN
NNNNNNNNNN NNNNNNNSND TTSSINSTTA TNTNTTNTTT TNTTTTTTTT NGLSNIVKPP
QRGKHITWSY DDIIEIHKRR HVLKNNAIEI FLGSGVPHKT YLFAFNKPSD RDIVYDLIMS
KPLPNRVDYA AEVHGNILKM SITKKWQSGL ISNFEYLMHL NTLAGRSFND LTQYPIFPFI
LRDYESEVLD LENPNTFRDF TKPMGAQDPK RLEKFIEKYN DLLEMNEKPY HYGSHYSNIG
SVLHFLVRLQ PFTSYFIDFQ GGRFDVPDRA FHSIAQSWNL SSSISNSDVK ELIPEFFYLS
DFLVNSNKFF MGIKQNGVKV DDVILPPWAH NDPRLFIKKH NEALECKYVS ENLHHWIDLL
FGYKQQGEAA VKAHNMFFPL TYEGAVDIDS IEDQLNRDAA VAQIHSYGQT PKQIFTKPHP
KKNWSKTIRL TQDSIFTKPE RLTSYIMFQY RSPIGSITIA NDSSPIHLTP QRILFFPDNN
KSISWGHWDQ NLRVNSIDTG KVLSIIEVLN DDIICGDITK NGRLFVTGGT AGTVKVWKRC
NNDGTIMTRK ERGDNLSLWS TLYGHTNSIL CVTVSQEYSI IVSGSKDSNC IIWDLNRLTY
INSLQHDHPV TCVQVSPTFG YIATFETNIY NKQNNNSGGG GSKNDNSING SGCLRLWSIN
GTLLAKQNFV NDRVNCMIFT STIQGVNTNL LITGMESGTI ILWNAWNLQK IRTLVSKSTI
TALAVSKDNT QLISGDINGL IECLSSRSFD GYSSIVLG
