LY66D_HUMAN
ID LY66D_HUMAN Reviewed; 133 AA.
AC O95868; A2BEY8; B0UXC1; B0V019; B0V1Y6; Q4VX50;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Lymphocyte antigen 6 complex locus protein G6d;
DE Short=Protein Ly6-D;
DE AltName: Full=Megakaryocyte-enhanced gene transcript 1 protein;
DE Flags: Precursor;
GN Name=LY6G6D; Synonyms=C6orf23, G6D, MEGT1, NG25;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=12079290; DOI=10.1006/geno.2002.6794;
RA Mallya M., Campbell R.D., Aguado B.;
RT "Transcriptional analysis of a novel cluster of LY-6 family members in the
RT human and mouse major histocompatibility complex: five genes with many
RT splice forms.";
RL Genomics 80:113-123(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=10384126;
RA Ribas G., Neville M., Wixon J.L., Cheng J., Campbell R.D.;
RT "Genes encoding three new members of the leukocyte antigen 6 superfamily
RT and a novel member of Ig superfamily, together with genes encoding the
RT regulatory nuclear chloride ion channel protein (hRNCC) and an N omega-N
RT omega-dimethylarginine dimethylaminohydrolase homologue, are found in a 30-
RT kb segment of the MHC class III region.";
RL J. Immunol. 163:278-287(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=14656967; DOI=10.1101/gr.1736803;
RA Xie T., Rowen L., Aguado B., Ahearn M.E., Madan A., Qin S., Campbell R.D.,
RA Hood L.;
RT "Analysis of the gene-dense major histocompatibility complex class III
RT region and its comparison to mouse.";
RL Genome Res. 13:2621-2636(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Shiina S., Tamiya G., Oka A., Inoko H.;
RT "Homo sapiens 2,229,817bp genomic DNA of 6p21.3 HLA class I region.";
RL Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=14574404; DOI=10.1038/nature02055;
RA Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L.,
RA Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R.,
RA Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D.,
RA Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H.,
RA Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J.,
RA Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V.,
RA Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J.,
RA Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E.,
RA Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J.,
RA French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J.,
RA Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C.,
RA Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A.,
RA Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R.,
RA Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M.,
RA Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K.,
RA Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R.,
RA Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M.,
RA Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A.,
RA Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L.,
RA Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I.,
RA Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y.,
RA Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E.,
RA Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A.,
RA Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W.,
RA Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M.,
RA West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J.,
RA Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M.,
RA Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I.,
RA Rogers J., Beck S.;
RT "The DNA sequence and analysis of human chromosome 6.";
RL Nature 425:805-811(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [8]
RP GLYCOSYLATION, SUBCELLULAR LOCATION, SUBUNIT, AND GPI-ANCHOR.
RX PubMed=17008713; DOI=10.1110/ps.062242606;
RA Mallya M., Campbell R.D., Aguado B.;
RT "Characterization of the five novel Ly-6 superfamily members encoded in the
RT MHC, and detection of cells expressing their potential ligands.";
RL Protein Sci. 15:2244-2256(2006).
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:17008713}.
CC -!- INTERACTION:
CC O95868; Q6FHY5: MEOX2; NbExp=3; IntAct=EBI-12382527, EBI-16439278;
CC O95868; P61601: NCALD; NbExp=3; IntAct=EBI-12382527, EBI-749635;
CC O95868; P62166: NCS1; NbExp=3; IntAct=EBI-12382527, EBI-746987;
CC O95868; Q9UHD9: UBQLN2; NbExp=3; IntAct=EBI-12382527, EBI-947187;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:17008713};
CC Lipid-anchor, GPI-anchor {ECO:0000269|PubMed:17008713}. Cell
CC projection, filopodium {ECO:0000269|PubMed:17008713}.
CC -!- TISSUE SPECIFICITY: Expressed in the adult lung, and in fetal liver,
CC lung, kidney, brain and spleen. {ECO:0000269|PubMed:12079290}.
CC -!- PTM: O-glycosylated. {ECO:0000269|PubMed:17008713}.
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DR EMBL; AJ315535; CAC85540.1; -; mRNA.
DR EMBL; AJ012008; CAB46082.1; -; Genomic_DNA.
DR EMBL; AF129756; AAD18077.1; -; Genomic_DNA.
DR EMBL; BA000025; BAB63380.1; -; Genomic_DNA.
DR EMBL; AL662899; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL670886; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BX248244; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL844216; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CR354443; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CR759787; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CR936239; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471081; EAX03486.1; -; Genomic_DNA.
DR EMBL; BC125138; AAI25139.1; -; mRNA.
DR CCDS; CCDS34404.1; -.
DR RefSeq; NP_067069.2; NM_021246.2.
DR PDB; 7S4G; X-ray; 2.20 A; G/I/J/K=95-103.
DR PDBsum; 7S4G; -.
DR AlphaFoldDB; O95868; -.
DR BioGRID; 121850; 12.
DR IntAct; O95868; 4.
DR STRING; 9606.ENSP00000364985; -.
DR GlyGen; O95868; 2 sites.
DR iPTMnet; O95868; -.
DR PhosphoSitePlus; O95868; -.
DR BioMuta; LY6G6D; -.
DR MassIVE; O95868; -.
DR PaxDb; O95868; -.
DR PeptideAtlas; O95868; -.
DR PRIDE; O95868; -.
DR ProteomicsDB; 51109; -.
DR Antibodypedia; 50491; 104 antibodies from 14 providers.
DR DNASU; 58530; -.
DR Ensembl; ENST00000375825.8; ENSP00000364985.3; ENSG00000244355.8.
DR Ensembl; ENST00000400080.7; ENSP00000382952.3; ENSG00000206402.10.
DR Ensembl; ENST00000419389.6; ENSP00000389619.2; ENSG00000226603.8.
DR Ensembl; ENST00000420378.6; ENSP00000397576.2; ENSG00000234443.9.
DR Ensembl; ENST00000434515.6; ENSP00000392651.2; ENSG00000235302.9.
DR Ensembl; ENST00000440095.6; ENSP00000390777.2; ENSG00000225993.9.
DR Ensembl; ENST00000444880.6; ENSP00000395722.2; ENSG00000236902.8.
DR GeneID; 58530; -.
DR KEGG; hsa:58530; -.
DR MANE-Select; ENST00000375825.8; ENSP00000364985.3; NM_021246.4; NP_067069.2.
DR UCSC; uc003nwf.1; human.
DR CTD; 58530; -.
DR DisGeNET; 58530; -.
DR GeneCards; LY6G6D; -.
DR HGNC; HGNC:13935; LY6G6D.
DR HPA; ENSG00000244355; Tissue enriched (skin).
DR MIM; 606038; gene.
DR neXtProt; NX_O95868; -.
DR OpenTargets; ENSG00000244355; -.
DR PharmGKB; PA37829; -.
DR VEuPathDB; HostDB:ENSG00000244355; -.
DR eggNOG; ENOG502SNF5; Eukaryota.
DR GeneTree; ENSGT00390000015960; -.
DR HOGENOM; CLU_1824727_0_0_1; -.
DR InParanoid; O95868; -.
DR OMA; QHQPACV; -.
DR OrthoDB; 1493078at2759; -.
DR PhylomeDB; O95868; -.
DR PathwayCommons; O95868; -.
DR Reactome; R-HSA-163125; Post-translational modification: synthesis of GPI-anchored proteins.
DR SignaLink; O95868; -.
DR BioGRID-ORCS; 58530; 11 hits in 1060 CRISPR screens.
DR GenomeRNAi; 58530; -.
DR Pharos; O95868; Tbio.
DR PRO; PR:O95868; -.
DR Proteomes; UP000005640; Chromosome 6.
DR RNAct; O95868; protein.
DR Bgee; ENSG00000244355; Expressed in ganglionic eminence and 77 other tissues.
DR ExpressionAtlas; O95868; baseline and differential.
DR Genevisible; O95868; HS.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009897; C:external side of plasma membrane; IEA:Ensembl.
DR GO; GO:0005576; C:extracellular region; TAS:Reactome.
DR GO; GO:0030175; C:filopodium; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0032991; C:protein-containing complex; IDA:UniProtKB.
DR GO; GO:0045202; C:synapse; IEA:GOC.
DR GO; GO:0030550; F:acetylcholine receptor inhibitor activity; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; IDA:UniProtKB.
DR GO; GO:0095500; P:acetylcholine receptor signaling pathway; IBA:GO_Central.
DR InterPro; IPR026524; LY6G6d/LY6G6f.
DR PANTHER; PTHR32286; PTHR32286; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; Cell projection; Disulfide bond; Glycoprotein;
KW GPI-anchor; Lipoprotein; Membrane; Reference proteome; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..104
FT /note="Lymphocyte antigen 6 complex locus protein G6d"
FT /id="PRO_0000323707"
FT PROPEP 105..133
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000323708"
FT DOMAIN 22..116
FT /note="UPAR/Ly6"
FT LIPID 104
FT /note="GPI-anchor amidated serine"
FT /evidence="ECO:0000255"
FT CARBOHYD 40
FT /note="O-linked (GalNAc...) threonine"
FT /evidence="ECO:0000255"
FT CARBOHYD 41
FT /note="O-linked (GalNAc...) threonine"
FT /evidence="ECO:0000255"
FT DISULFID 27..35
FT /evidence="ECO:0000250"
FT DISULFID 42..71
FT /evidence="ECO:0000250"
FT DISULFID 77..96
FT /evidence="ECO:0000250"
FT VARIANT 9
FT /note="L -> V (in dbSNP:rs3749952)"
FT /id="VAR_039564"
FT VARIANT 34
FT /note="S -> T (in dbSNP:rs9267550)"
FT /id="VAR_039565"
SQ SEQUENCE 133 AA; 13691 MW; C5CE88EEAFE5F2FD CRC64;
MKPQFVGILL SSLLGAALGN RMRCYNCGGS PSSSCKEAVT TCGEGRPQPG LEQIKLPGNP
PVTLIHQHPA CVAAHHCNQV ETESVGDVTY PAHRDCYLGD LCNSAVASHV APAGILAAAA
TALTCLLPGL WSG
