LY6A_MOUSE
ID LY6A_MOUSE Reviewed; 134 AA.
AC P05533; Q8BPD0;
DT 01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1988, sequence version 1.
DT 03-AUG-2022, entry version 172.
DE RecName: Full=Lymphocyte antigen 6A-2/6E-1;
DE Short=Ly-6A.2/Ly-6E.1;
DE AltName: Full=Stem cell antigen 1;
DE Short=SCA-1;
DE AltName: Full=T-cell-activating protein;
DE Short=TAP;
DE Flags: Precursor;
GN Name=Ly6a; Synonyms=Ly6;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2822573; DOI=10.1007/bf00343712;
RA Palfree R.G.E., Leclair K.P., Bothwell A.L.M., Haemmerling U.;
RT "cDNA characterization of an Ly-6.2 gene expressed in BW5147 tumor cells.";
RL Immunogenetics 26:389-391(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2895473; DOI=10.1073/pnas.85.7.2255;
RA Reiser H., Coligan J., Benacerraf B., Rock K.L.;
RT "Cloning and expression of a cDNA for the T-cell-activating protein TAP.";
RL Proc. Natl. Acad. Sci. U.S.A. 85:2255-2259(1988).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (LY-6E.1).
RX PubMed=3028776; DOI=10.1002/j.1460-2075.1986.tb04633.x;
RA Leclair K.P., Palfree R.G.E., Flood P.M., Hammerling U., Bothwell A.L.M.;
RT "Isolation of a murine Ly-6 cDNA reveals a new multigene family.";
RL EMBO J. 5:3227-3234(1986).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=BALB/cJ;
RX PubMed=1697928; DOI=10.1128/mcb.10.10.5150-5159.1990;
RA Khan K., Dad Lindwall G., Maher S.E., Bothwell A.L.M.;
RT "Characterization of promoter elements of an interferon-inducible Ly-6E/A
RT differentiation antigen, which is expressed on activated T cells and
RT hematopoietic stem cells.";
RL Mol. Cell. Biol. 10:5150-5159(1990).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1315719; DOI=10.1007/bf00179799;
RA Stanford W.L., Bruyns E., Snodgrass H.R.;
RT "The isolation and sequence of the chromosomal gene and regulatory regions
RT of Ly-6A.2.";
RL Immunogenetics 35:408-411(1992).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1709198;
RA McGrew J.T., Rock K.L.;
RT "Isolation, expression, and sequence of the TAP/Ly-6A.2 chromosomal gene.";
RL J. Immunol. 146:3633-3638(1991).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Extraembryonic tissue, and Placenta;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [9]
RP PROTEIN SEQUENCE OF 27-49, AND GLYCOSYLATION.
RX PubMed=3033645; DOI=10.1073/pnas.84.10.3370;
RA Reiser H., Coligan J., Benacerraf B., Rock K.L.;
RT "Biosynthesis, glycosylation, and partial N-terminal amino acid sequence of
RT the T-cell-activating protein TAP.";
RL Proc. Natl. Acad. Sci. U.S.A. 84:3370-3374(1987).
RN [10]
RP TISSUE SPECIFICITY.
RX PubMed=2660142; DOI=10.1073/pnas.86.12.4634;
RA van de Rijn M., Heimfeld S., Spangrude G.J., Weissman I.L.;
RT "Mouse hematopoietic stem-cell antigen Sca-1 is a member of the Ly-6
RT antigen family.";
RL Proc. Natl. Acad. Sci. U.S.A. 86:4634-4638(1989).
RN [11]
RP TISSUE SPECIFICITY.
RX PubMed=15930324; DOI=10.1095/biolreprod.105.040303;
RA van Bragt M.P., Ciliberti N., Stanford W.L., de Rooij D.G., van Pelt A.M.;
RT "LY6A/E (SCA-1) expression in the mouse testis.";
RL Biol. Reprod. 73:634-638(2005).
RN [12]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brown adipose tissue, Heart, Kidney, Lung, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: T-cell activation.
CC -!- INTERACTION:
CC P05533; Q64729: Tgfbr1; NbExp=2; IntAct=EBI-11600492, EBI-2899393;
CC -!- SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor.
CC -!- TISSUE SPECIFICITY: Widely expressed. {ECO:0000269|PubMed:15930324,
CC ECO:0000269|PubMed:2660142}.
CC -!- PTM: O-glycosylated. Not N-glycosylated. {ECO:0000269|PubMed:3033645}.
CC -!- PTM: Not phosphorylated.
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DR EMBL; M18184; AAA39463.1; -; mRNA.
DR EMBL; J03636; AAA40163.1; -; mRNA.
DR EMBL; X04653; CAA28351.1; -; mRNA.
DR EMBL; M37707; AAA39467.1; -; Genomic_DNA.
DR EMBL; M74013; AAA39464.1; -; Genomic_DNA.
DR EMBL; M73552; AAA39465.1; -; Genomic_DNA.
DR EMBL; M59713; AAA40164.1; -; Genomic_DNA.
DR EMBL; AK076200; BAC36250.1; -; mRNA.
DR EMBL; BC002070; AAH02070.1; -; mRNA.
DR CCDS; CCDS27540.1; -.
DR PIR; A25708; A25708.
DR PIR; A35921; A32506.
DR RefSeq; NP_001258345.1; NM_001271416.1.
DR RefSeq; NP_001258346.1; NM_001271417.1.
DR RefSeq; NP_001258347.1; NM_001271418.1.
DR RefSeq; NP_001258348.1; NM_001271419.1.
DR RefSeq; NP_001258375.1; NM_001271446.1.
DR RefSeq; NP_034868.1; NM_010738.3.
DR AlphaFoldDB; P05533; -.
DR SMR; P05533; -.
DR BioGRID; 225606; 1.
DR DIP; DIP-59628N; -.
DR IntAct; P05533; 2.
DR STRING; 10090.ENSMUSP00000140287; -.
DR SwissPalm; P05533; -.
DR EPD; P05533; -.
DR jPOST; P05533; -.
DR MaxQB; P05533; -.
DR PaxDb; P05533; -.
DR PeptideAtlas; P05533; -.
DR PRIDE; P05533; -.
DR ProteomicsDB; 290196; -.
DR DNASU; 110454; -.
DR Ensembl; ENSMUST00000023248; ENSMUSP00000023248; ENSMUSG00000075602.
DR Ensembl; ENSMUST00000186526; ENSMUSP00000140998; ENSMUSG00000075602.
DR Ensembl; ENSMUST00000187171; ENSMUSP00000140099; ENSMUSG00000075602.
DR Ensembl; ENSMUST00000187994; ENSMUSP00000140287; ENSMUSG00000075602.
DR Ensembl; ENSMUST00000189068; ENSMUSP00000140638; ENSMUSG00000075602.
DR GeneID; 110454; -.
DR KEGG; mmu:110454; -.
DR UCSC; uc007wgk.2; mouse.
DR CTD; 110454; -.
DR MGI; MGI:107527; Ly6a.
DR VEuPathDB; HostDB:ENSMUSG00000075602; -.
DR eggNOG; ENOG502TD4F; Eukaryota.
DR GeneTree; ENSGT00940000154560; -.
DR HOGENOM; CLU_106772_0_0_1; -.
DR InParanoid; P05533; -.
DR OMA; KISCCKE; -.
DR OrthoDB; 1592016at2759; -.
DR PhylomeDB; P05533; -.
DR TreeFam; TF337757; -.
DR BioGRID-ORCS; 110454; 2 hits in 51 CRISPR screens.
DR ChiTaRS; Ly6a; mouse.
DR PRO; PR:P05533; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; P05533; protein.
DR Bgee; ENSMUSG00000075602; Expressed in gastrula and 238 other tissues.
DR ExpressionAtlas; P05533; baseline and differential.
DR Genevisible; P05533; MM.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009897; C:external side of plasma membrane; IDA:MGI.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0045202; C:synapse; IEA:GOC.
DR GO; GO:0033130; F:acetylcholine receptor binding; IBA:GO_Central.
DR GO; GO:0030550; F:acetylcholine receptor inhibitor activity; IBA:GO_Central.
DR GO; GO:0095500; P:acetylcholine receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0009617; P:response to bacterium; IEP:MGI.
DR CDD; cd00117; LU; 1.
DR Gene3D; 2.10.60.10; -; 1.
DR InterPro; IPR018363; CD59_antigen_CS.
DR InterPro; IPR016054; LY6_UPA_recep-like.
DR InterPro; IPR045860; Snake_toxin-like_sf.
DR Pfam; PF00021; UPAR_LY6; 1.
DR SMART; SM00134; LU; 1.
DR SUPFAM; SSF57302; SSF57302; 1.
DR PROSITE; PS00983; LY6_UPAR; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Direct protein sequencing; Disulfide bond; Glycoprotein;
KW GPI-anchor; Lipoprotein; Membrane; Reference proteome; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000269|PubMed:3033645"
FT CHAIN 27..112
FT /note="Lymphocyte antigen 6A-2/6E-1"
FT /id="PRO_0000036130"
FT PROPEP 113..134
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000036131"
FT DOMAIN 27..119
FT /note="UPAR/Ly6"
FT LIPID 112
FT /note="GPI-anchor amidated glycine"
FT /evidence="ECO:0000255"
FT DISULFID 29..53
FT /evidence="ECO:0000250"
FT DISULFID 32..41
FT /evidence="ECO:0000250"
FT DISULFID 46..74
FT /evidence="ECO:0000250"
FT DISULFID 78..98
FT /evidence="ECO:0000250"
FT DISULFID 99..104
FT /evidence="ECO:0000250"
FT VARIANT 63
FT /note="D -> G (in Ly-6E.1)"
FT VARIANT 106
FT /note="V -> A (in Ly-6E.1)"
FT CONFLICT 32
FT /note="C -> S (in Ref. 7; BAC36250)"
FT /evidence="ECO:0000305"
FT CONFLICT 56
FT /note="Q -> H (in Ref. 7; BAC36250)"
FT /evidence="ECO:0000305"
FT CONFLICT 59
FT /note="A -> T (in Ref. 7; BAC36250)"
FT /evidence="ECO:0000305"
FT CONFLICT 101
FT /note="E -> Q (in Ref. 7; BAC36250)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 134 AA; 14377 MW; 883C771EB3430315 CRC64;
MDTSHTTKSC LLILLVALLC AERAQGLECY QCYGVPFETS CPSITCPYPD GVCVTQEAAV
IVDSQTRKVK NNLCLPICPP NIESMEILGT KVNVKTSCCQ EDLCNVAVPN GGSTWTMAGV
LLFSLSSVLL QTLL
