LY6K_HUMAN
ID LY6K_HUMAN Reviewed; 165 AA.
AC Q17RY6; G3V116; O15227; Q9BVD7;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 20-MAY-2008, sequence version 2.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Lymphocyte antigen 6K {ECO:0000305};
DE Short=Ly-6K {ECO:0000303|PubMed:12516096};
DE Flags: Precursor;
GN Name=LY6K {ECO:0000312|HGNC:HGNC:24225}; Synonyms=CO16 {ECO:0000303|Ref.1};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Fibroblast;
RA Machl A.W., Planitzer S.A., Rueckels M., Kubbies M.;
RT "Identification and characterization of a novel cDNA not expressed in
RT Fanconi anemia fibroblasts and B-cell lines.";
RL Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16421571; DOI=10.1038/nature04406;
RA Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M.,
RA Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L.,
RA Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S.,
RA Asakawa T., Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A.,
RA Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III,
RA Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K.,
RA Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P.,
RA Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H.,
RA Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B.,
RA O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K.,
RA Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L.,
RA Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G.,
RA Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W.,
RA Platzer M., Shimizu N., Lander E.S.;
RT "DNA sequence and analysis of human chromosome 8.";
RL Nature 439:331-335(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain, and Cervix;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-104 (ISOFORM 2).
RG The Cancer Genome Anatomy Project (CGAP) at the National Cancer Institute;
RL Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=12516096; DOI=10.1002/ijc.10903;
RA de Nooij-van Dalen A.G., van Dongen G.A.M.S., Smeets S.J.,
RA Nieuwenhuis E.J.C., Stigter-van Walsum M., Snow G.B., Brakenhoff R.H.;
RT "Characterization of the human Ly-6 antigens, the newly annotated member
RT Ly-6K included, as molecular markers for head-and-neck squamous cell
RT carcinoma.";
RL Int. J. Cancer 103:768-774(2003).
RN [7]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=18089789; DOI=10.1158/0008-5472.can-07-3243;
RA Ishikawa N., Takano A., Yasui W., Inai K., Nishimura H., Ito H., Miyagi Y.,
RA Nakayama H., Fujita M., Hosokawa M., Tsuchiya E., Kohno N., Nakamura Y.,
RA Daigo Y.;
RT "Cancer-testis antigen lymphocyte antigen 6 complex locus K is a serologic
RT biomarker and a therapeutic target for lung and esophageal carcinomas.";
RL Cancer Res. 67:11601-11611(2007).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
CC -!- FUNCTION: Required for sperm migration into the oviduct and male
CC fertility by controlling binding of sperm to zona pellucida (By
CC similarity). May play a role in cell growth (PubMed:18089789).
CC {ECO:0000250|UniProtKB:Q9CWP4, ECO:0000269|PubMed:18089789}.
CC -!- SUBUNIT: Interacts with TEX101. {ECO:0000250|UniProtKB:Q9CWP4}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:18089789}. Cytoplasm
CC {ECO:0000269|PubMed:18089789}. Cell membrane
CC {ECO:0000250|UniProtKB:Q9CWP4}; Lipid-anchor, GPI-anchor
CC {ECO:0000250|UniProtKB:Q9CWP4}. Cytoplasmic vesicle, secretory vesicle,
CC acrosome {ECO:0000250|UniProtKB:Q9CWP4}. Membrane raft
CC {ECO:0000250|UniProtKB:Q9CWP4}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q17RY6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q17RY6-2; Sequence=VSP_045602, VSP_045603;
CC -!- TISSUE SPECIFICITY: Specifically expressed in testis (at protein
CC level). {ECO:0000269|PubMed:12516096, ECO:0000269|PubMed:18089789}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH01291.2; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAI17143.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAI17145.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAA04692.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAA04692.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=EAW82311.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AJ001348; CAA04692.1; ALT_SEQ; mRNA.
DR EMBL; AC108002; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471162; EAW82311.1; ALT_INIT; Genomic_DNA.
DR EMBL; BC001291; AAH01291.2; ALT_INIT; mRNA.
DR EMBL; BC117142; AAI17143.1; ALT_INIT; mRNA.
DR EMBL; BC117144; AAI17145.1; ALT_INIT; mRNA.
DR EMBL; AI740834; -; NOT_ANNOTATED_CDS; mRNA.
DR CCDS; CCDS59114.1; -. [Q17RY6-2]
DR CCDS; CCDS6385.2; -. [Q17RY6-1]
DR RefSeq; NP_001153826.1; NM_001160354.1.
DR RefSeq; NP_001153827.1; NM_001160355.1. [Q17RY6-2]
DR RefSeq; NP_059997.3; NM_017527.3. [Q17RY6-1]
DR AlphaFoldDB; Q17RY6; -.
DR BioGRID; 120124; 1.
DR STRING; 9606.ENSP00000292430; -.
DR GlyGen; Q17RY6; 1 site.
DR iPTMnet; Q17RY6; -.
DR PhosphoSitePlus; Q17RY6; -.
DR BioMuta; LY6K; -.
DR DMDM; 189028864; -.
DR EPD; Q17RY6; -.
DR jPOST; Q17RY6; -.
DR MassIVE; Q17RY6; -.
DR MaxQB; Q17RY6; -.
DR PaxDb; Q17RY6; -.
DR PeptideAtlas; Q17RY6; -.
DR PRIDE; Q17RY6; -.
DR ProteomicsDB; 32227; -.
DR ProteomicsDB; 61174; -. [Q17RY6-1]
DR Antibodypedia; 3032; 116 antibodies from 27 providers.
DR CPTC; Q17RY6; 3 antibodies.
DR DNASU; 54742; -.
DR Ensembl; ENST00000292430.10; ENSP00000292430.6; ENSG00000160886.13. [Q17RY6-1]
DR Ensembl; ENST00000519387.1; ENSP00000429695.1; ENSG00000160886.13. [Q17RY6-2]
DR GeneID; 54742; -.
DR KEGG; hsa:54742; -.
DR MANE-Select; ENST00000292430.10; ENSP00000292430.6; NM_017527.4; NP_059997.3.
DR UCSC; uc011ljv.3; human. [Q17RY6-1]
DR CTD; 54742; -.
DR DisGeNET; 54742; -.
DR GeneCards; LY6K; -.
DR HGNC; HGNC:24225; LY6K.
DR HPA; ENSG00000160886; Group enriched (esophagus, testis).
DR MIM; 615093; gene.
DR neXtProt; NX_Q17RY6; -.
DR OpenTargets; ENSG00000160886; -.
DR PharmGKB; PA134987940; -.
DR VEuPathDB; HostDB:ENSG00000160886; -.
DR eggNOG; ENOG502TDVS; Eukaryota.
DR GeneTree; ENSGT00940000159966; -.
DR HOGENOM; CLU_105666_0_0_1; -.
DR InParanoid; Q17RY6; -.
DR OMA; YCNLQGP; -.
DR OrthoDB; 1586315at2759; -.
DR PhylomeDB; Q17RY6; -.
DR TreeFam; TF336908; -.
DR PathwayCommons; Q17RY6; -.
DR Reactome; R-HSA-163125; Post-translational modification: synthesis of GPI-anchored proteins.
DR BioGRID-ORCS; 54742; 6 hits in 1064 CRISPR screens.
DR GenomeRNAi; 54742; -.
DR Pharos; Q17RY6; Tbio.
DR PRO; PR:Q17RY6; -.
DR Proteomes; UP000005640; Chromosome 8.
DR RNAct; Q17RY6; protein.
DR Bgee; ENSG00000160886; Expressed in right testis and 131 other tissues.
DR ExpressionAtlas; Q17RY6; baseline and differential.
DR Genevisible; Q17RY6; HS.
DR GO; GO:0001669; C:acrosomal vesicle; ISS:UniProtKB.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005576; C:extracellular region; TAS:Reactome.
DR GO; GO:0045121; C:membrane raft; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0007339; P:binding of sperm to zona pellucida; IBA:GO_Central.
DR Gene3D; 2.10.60.10; -; 1.
DR InterPro; IPR045860; Snake_toxin-like_sf.
DR SUPFAM; SSF57302; SSF57302; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Cytoplasm; Cytoplasmic vesicle;
KW Glycoprotein; GPI-anchor; Lipoprotein; Membrane; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..17
FT /evidence="ECO:0000255"
FT CHAIN 18..138
FT /note="Lymphocyte antigen 6K"
FT /id="PRO_0000337084"
FT PROPEP 139..165
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000337085"
FT DOMAIN 47..141
FT /note="UPAR/Ly6"
FT LIPID 138
FT /note="GPI-anchor amidated glycine"
FT /evidence="ECO:0000255"
FT CARBOHYD 20
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 73..105
FT /note="KIFPRFFMVAKQCSAGCAAMERPKPEEKRFLLE -> TVLRWLCSDGETQAR
FT GEAVSPGRAHALLLPQVL (in isoform 2)"
FT /evidence="ECO:0000303|Ref.5"
FT /id="VSP_045602"
FT VAR_SEQ 106..165
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.5"
FT /id="VSP_045603"
FT CONFLICT 29
FT /note="E -> Q (in Ref. 1; CAA04692)"
FT /evidence="ECO:0000305"
FT CONFLICT 41
FT /note="V -> A (in Ref. 1; CAA04692)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 165 AA; 18673 MW; BA75B436BE91363D CRC64;
MALLALLLVV ALPRVWTDAN LTARQRDPED SQRTDEGDNR VWCHVCEREN TFECQNPRRC
KWTEPYCVIA AVKIFPRFFM VAKQCSAGCA AMERPKPEEK RFLLEEPMPF FYLKCCKIRY
CNLEGPPINS SVFKEYAGSM GESCGGLWLA ILLLLASIAA GLSLS
