LY6K_MOUSE
ID LY6K_MOUSE Reviewed; 154 AA.
AC Q9CWP4;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Lymphocyte antigen 6K {ECO:0000250|UniProtKB:Q17RY6};
DE Short=Ly-6K {ECO:0000250|UniProtKB:Q17RY6};
DE Flags: Precursor;
GN Name=Ly6k {ECO:0000312|MGI:MGI:1923736};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP SUBCELLULAR LOCATION, AND INTERACTION WITH TEX101.
RX PubMed=18503752; DOI=10.1016/j.bbrc.2008.05.088;
RA Yoshitake H., Tsukamoto H., Maruyama-Fukushima M., Takamori K., Ogawa H.,
RA Araki Y.;
RT "TEX101, a germ cell-marker glycoprotein, is associated with lymphocyte
RT antigen 6 complex locus k within the mouse testis.";
RL Biochem. Biophys. Res. Commun. 372:277-282(2008).
RN [4]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=20920470; DOI=10.1016/j.bbrc.2010.09.117;
RA Maruyama M., Yoshitake H., Tsukamoto H., Takamori K., Araki Y.;
RT "Molecular expression of Ly6k, a putative glycosylphosphatidyl-inositol-
RT anchored membrane protein on the mouse testicular germ cells.";
RL Biochem. Biophys. Res. Commun. 402:75-81(2010).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [6]
RP TISSUE SPECIFICITY, INTERACTION WITH ADAM3 AND TEX101, DISRUPTION
RP PHENOTYPE, AND FUNCTION.
RX PubMed=24501175; DOI=10.1095/biolreprod.113.112888;
RA Fujihara Y., Okabe M., Ikawa M.;
RT "GPI-anchored protein complex, LY6K/TEX101, is required for sperm migration
RT into the oviduct and male fertility in mice.";
RL Biol. Reprod. 90:60-60(2014).
RN [7]
RP TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RX PubMed=27005865; DOI=10.1038/srep23616;
RA Endo S., Yoshitake H., Tsukamoto H., Matsuura H., Kato K., Sakuraba M.,
RA Takamori K., Fujiwara H., Takeda S., Araki Y.;
RT "TEX101, a glycoprotein essential for sperm fertility, is required for
RT stable expression of Ly6k on testicular germ cells.";
RL Sci. Rep. 6:23616-23616(2016).
CC -!- FUNCTION: Required for sperm migration into the oviduct and male
CC fertility by controlling binding of sperm to zona pellucida
CC (PubMed:24501175). May play a role in cell growth (By similarity).
CC {ECO:0000250|UniProtKB:Q17RY6, ECO:0000269|PubMed:24501175}.
CC -!- SUBUNIT: Interacts with ADAM3 and TEX101. {ECO:0000269|PubMed:18503752,
CC ECO:0000269|PubMed:24501175}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}. Cytoplasm {ECO:0000250}.
CC Cell membrane {ECO:0000269|PubMed:27005865}; Lipid-anchor, GPI-anchor
CC {ECO:0000305}. Cytoplasmic vesicle, secretory vesicle, acrosome
CC {ECO:0000269|PubMed:27005865}. Membrane raft
CC {ECO:0000269|PubMed:18503752}.
CC -!- TISSUE SPECIFICITY: Strongly expressed in testes and weakly expressed
CC in the epididymis, ovary, and uterus (PubMed:20920470). Expressed in
CC testicular germ cells (TGCs) (PubMed:24501175). Expressed in the
CC testicular seminiferous tubules, in spermatocytes, spermatids, and
CC testicular spermatozoa (PubMed:27005865). {ECO:0000269|PubMed:20920470,
CC ECO:0000269|PubMed:24501175, ECO:0000269|PubMed:27005865}.
CC -!- DEVELOPMENTAL STAGE: Weakly expressed in testes from 18 day postcoitus
CC to 1 day postpartum (dpp), with a plateau starting around 8 dpp; and
CC testicular expression shows two-peak expression at around 14 dpp and 24
CC dpp, then exhibits stable expression from 6-week after birth onward.
CC {ECO:0000269|PubMed:20920470}.
CC -!- DISRUPTION PHENOTYPE: Knockout Ly6k mice are viable and show no overt
CC developmental abnormalities. Males are infertile.
CC {ECO:0000269|PubMed:24501175}.
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DR EMBL; AK010485; BAB26976.1; -; mRNA.
DR EMBL; BC049723; AAH49723.1; -; mRNA.
DR CCDS; CCDS27532.1; -.
DR RefSeq; NP_083903.1; NM_029627.2.
DR AlphaFoldDB; Q9CWP4; -.
DR SMR; Q9CWP4; -.
DR STRING; 10090.ENSMUSP00000052599; -.
DR iPTMnet; Q9CWP4; -.
DR PhosphoSitePlus; Q9CWP4; -.
DR PaxDb; Q9CWP4; -.
DR PRIDE; Q9CWP4; -.
DR ProteomicsDB; 295732; -.
DR Antibodypedia; 3032; 116 antibodies from 27 providers.
DR DNASU; 76486; -.
DR Ensembl; ENSMUST00000060301; ENSMUSP00000052599; ENSMUSG00000044678.
DR Ensembl; ENSMUST00000168815; ENSMUSP00000132129; ENSMUSG00000044678.
DR GeneID; 76486; -.
DR KEGG; mmu:76486; -.
DR UCSC; uc007wga.1; mouse.
DR CTD; 54742; -.
DR MGI; MGI:1923736; Ly6k.
DR VEuPathDB; HostDB:ENSMUSG00000044678; -.
DR eggNOG; ENOG502TDVS; Eukaryota.
DR GeneTree; ENSGT00940000159966; -.
DR HOGENOM; CLU_105666_0_0_1; -.
DR InParanoid; Q9CWP4; -.
DR OMA; YCNLQGP; -.
DR OrthoDB; 1586315at2759; -.
DR PhylomeDB; Q9CWP4; -.
DR TreeFam; TF336908; -.
DR Reactome; R-MMU-163125; Post-translational modification: synthesis of GPI-anchored proteins.
DR BioGRID-ORCS; 76486; 2 hits in 73 CRISPR screens.
DR PRO; PR:Q9CWP4; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; Q9CWP4; protein.
DR Bgee; ENSMUSG00000044678; Expressed in spermatocyte and 71 other tissues.
DR Genevisible; Q9CWP4; MM.
DR GO; GO:0001669; C:acrosomal vesicle; IDA:UniProtKB.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009986; C:cell surface; IDA:MGI.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0045121; C:membrane raft; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0007339; P:binding of sperm to zona pellucida; IMP:MGI.
DR GO; GO:0030317; P:flagellated sperm motility; IMP:MGI.
DR CDD; cd00117; LU; 1.
DR Gene3D; 2.10.60.10; -; 1.
DR InterPro; IPR016054; LY6_UPA_recep-like.
DR InterPro; IPR045860; Snake_toxin-like_sf.
DR Pfam; PF00021; UPAR_LY6; 1.
DR SMART; SM00134; LU; 1.
DR SUPFAM; SSF57302; SSF57302; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Cytoplasm; Cytoplasmic vesicle; Glycoprotein; GPI-anchor;
KW Lipoprotein; Membrane; Reference proteome; Secreted; Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..123
FT /note="Lymphocyte antigen 6K"
FT /id="PRO_0000318201"
FT PROPEP 124..154
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000337086"
FT DOMAIN 21..117
FT /note="UPAR/Ly6"
FT LIPID 123
FT /note="GPI-anchor amidated glycine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 154 AA; 17134 MW; F756FBB2B3B400C6 CRC64;
MAFLVALLVV LGLQLVQSNA LTCHVCEAQN SYACSNPSQC PGEKKFCLLA VTRIFERFFY
VSKQCTRRCP TPVVSPPSTN PPSEPKEFLI EKPMPFLFYK CCQWDSCNGE GPPTDQLLKE
QPGKASGRRH RYIELLLTGF MVLTANGLSA LCLL
