ID   LYC3_LYCSI              Reviewed;          26 AA.
AC   P0C2U8;
DT   01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2007, sequence version 1.
DT   25-MAY-2022, entry version 31.
DE   RecName: Full=M-lycotoxin-Ls2a;
DE            Short=M-LCTX-Ls2a;
DE   AltName: Full=Lycocitin-3;
OS   Lycosa singoriensis (Wolf spider) (Aranea singoriensis).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Lycosoidea; Lycosidae; Lycosa.
OX   NCBI_TaxID=434756;
RN   [1]
RP   PROTEIN SEQUENCE, AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=14991689; DOI=10.1002/jms.577;
RA   Budnik B.A., Olsen J.V., Egorov T.A., Anisimova V.E., Galkina T.G.,
RA   Musolyamov A.K., Grishin E.V., Zubarev R.A.;
RT   "De novo sequencing of antimicrobial peptides isolated from the venom
RT   glands of the wolf spider Lycosa singoriensis.";
RL   J. Mass Spectrom. 39:193-201(2004).
CC   -!- FUNCTION: Forms pore that permeabilize the cell membrane. Promotes
CC       efflux of calcium from synaptosomes, causes hemolysis, and dissipates
CC       voltage gradients across muscle membrane. Potently inhibits the growth
CC       of bacteria and yeast. May function both in the prey capture strategy
CC       as well as protection from infectious organisms arising from prey
CC       ingestion (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted. Target cell membrane {ECO:0000250}.
CC       Note=Forms a membrane channel in the prey. {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- MASS SPECTROMETRY: Mass=3149.75; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:14991689};
CC   -!- SIMILARITY: Belongs to the cationic peptide 04 (cupiennin) family. 05
CC       subfamily.
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DR   AlphaFoldDB; P0C2U8; -.
DR   ArachnoServer; AS000064; M-lycotoxin-Ls2a.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR   GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Cytolysis; Direct protein sequencing; Hemolysis;
KW   Ion transport; Membrane; Neurotoxin; Secreted; Target cell membrane;
KW   Target membrane; Toxin; Transmembrane; Transport.
FT   PEPTIDE         1..26
FT                   /note="M-lycotoxin-Ls2a"
FT                   /id="PRO_0000285258"
SQ   SEQUENCE   26 AA;  3150 MW;  864D694C856DB8EC CRC64;
     KIKWFKTMKS LAKFLAKEQM KKHLGE