ID   LYOPH_LYOSH             Reviewed;          50 AA.
AC   C0HLZ4;
DT   25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT   25-MAY-2022, sequence version 1.
DT   03-AUG-2022, entry version 2.
DE   RecName: Full=Ribosome-inactivating protein lyophyllin {ECO:0000303|PubMed:11556814};
DE            EC=3.2.2.22 {ECO:0000269|PubMed:11556814, ECO:0000269|PubMed:34769028};
DE   Flags: Fragments;
OS   Lyophyllum shimeji (Hon-shimeji) (Tricholoma shimeji).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC   Agaricomycetidae; Agaricales; Lyophyllaceae; Lyophyllum.
OX   NCBI_TaxID=47721 {ECO:0000303|PubMed:11556814};
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE OF 1-40, FUNCTION, AND CATALYTIC ACTIVITY.
RC   TISSUE=Fruiting body {ECO:0000303|PubMed:11556814};
RX   PubMed=11556814; DOI=10.1006/abbi.2001.2506;
RA   Lam S.K., Ng T.B.;
RT   "First simultaneous isolation of a ribosome inactivating protein and an
RT   antifungal protein from a mushroom (Lyophyllum shimeji) together with
RT   evidence for synergism of their antifungal effects.";
RL   Arch. Biochem. Biophys. 393:271-280(2001).
RN   [2] {ECO:0000305}
RP   PROTEIN SEQUENCE OF 1-30, AND FUNCTION.
RC   TISSUE=Fruiting body {ECO:0000303|PubMed:19568748};
RX   PubMed=19568748; DOI=10.1007/s00253-009-2048-y;
RA   Chan W.Y., Ng T.B., Lam J.S., Wong J.H., Chu K.T., Ngai P.H., Lam S.K.,
RA   Wang H.X.;
RT   "The mushroom ribosome-inactivating protein lyophyllin exerts deleterious
RT   effects on mouse embryonic development in vitro.";
RL   Appl. Microbiol. Biotechnol. 85:985-993(2010).
RN   [3] {ECO:0000305}
RP   PROTEIN SEQUENCE OF 1-18 AND 41-50, FUNCTION, AND CATALYTIC ACTIVITY.
RC   TISSUE=Fruiting body {ECO:0000303|PubMed:34769028};
RX   PubMed=34769028; DOI=10.3390/ijms222111598;
RA   Lu J.Q., Shi W.W., Xiao M.J., Tang Y.S., Zheng Y.T., Shaw P.C.;
RT   "Lyophyllin, a Mushroom Protein from the Peptidase M35 Superfamily Is an
RT   RNA N-Glycosidase.";
RL   Int. J. Mol. Sci. 22:0-0(2021).
CC   -!- FUNCTION: N-glycosylase that inhibits protein synthesis by depurinating
CC       ribosomal rRNA, and thus acts as a ribosomal inactivating protein (RIP)
CC       (PubMed:34769028, PubMed:11556814). Has adenine polynucleotide
CC       glycosidase activity on the poly(A) substrate A30-ssDNA
CC       (PubMed:11556814). Inhibits cell-free translation in rabbit
CC       reticulocyte lysate system with an IC(50) of 1 nM (PubMed:11556814,
CC       PubMed:34769028). May function in the defense response to pathogens
CC       (PubMed:11556814). Displays antifungal activity against C.comatus and
CC       P.piricola, but not against R.solani, M.arachidicola and C.gossypii
CC       (PubMed:11556814). Inhibits mycelial growth in P.piricola with an
CC       IC(50) of 2.5 uM (PubMed:11556814). Has cytotoxic activity against the
CC       human cancer cell lines Hela, HepG2, and JAR, with IC(50) of 358.8,
CC       489.8, and 926.9 nM respectively (PubMed:34769028). It also inhibits
CC       HIV-1 reverse transcriptase activity (IC(50)=7.9 nM) and disrupts mouse
CC       embryonic development (PubMed:11556814, PubMed:19568748).
CC       {ECO:0000269|PubMed:11556814, ECO:0000269|PubMed:19568748,
CC       ECO:0000269|PubMed:34769028}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of the N-glycosidic bond at one specific
CC         adenosine on the 28S rRNA.; EC=3.2.2.22;
CC         Evidence={ECO:0000269|PubMed:11556814, ECO:0000269|PubMed:34769028};
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DR   GO; GO:0030598; F:rRNA N-glycosylase activity; IDA:UniProtKB.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0050832; P:defense response to fungus; IDA:UniProtKB.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   GO; GO:0017148; P:negative regulation of translation; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.390.10; -; 1.
DR   InterPro; IPR024079; MetalloPept_cat_dom_sf.
PE   1: Evidence at protein level;
KW   Antimicrobial; Direct protein sequencing; Fungicide; Hydrolase;
KW   Protein synthesis inhibitor; Toxin.
FT   CHAIN           1..50
FT                   /note="Ribosome-inactivating protein lyophyllin"
FT                   /id="PRO_0000455337"
FT   NON_CONS        40..41
FT                   /evidence="ECO:0000303|PubMed:34769028"
FT   NON_TER         50
FT                   /evidence="ECO:0000303|PubMed:34769028"
SQ   SEQUENCE   50 AA;  5358 MW;  CFDA7312645CAF2D CRC64;
     ITFQGCSPAR QTVITNAITR ARADVRAAVS ALPTKAPVST FSTWFGVYND