LYPD1_HUMAN
ID LYPD1_HUMAN Reviewed; 141 AA.
AC Q8N2G4; H7BXW6; Q6ZP52; Q6ZWI4; Q96AC2;
DT 07-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 07-MAR-2006, sequence version 2.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Ly6/PLAUR domain-containing protein 1;
DE AltName: Full=Putative HeLa tumor suppressor;
DE Short=PHTS;
DE Flags: Precursor;
GN Name=LYPD1; Synonyms=LYPDC1; ORFNames=PSEC0181, UNQ3079/PRO9917;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Ovary;
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
RC TISSUE=Brain cortex, Fetal brain, and Kidney;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Ovary tumor;
RX PubMed=16303743; DOI=10.1093/dnares/12.2.117;
RA Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J.,
RA Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S.,
RA Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y.,
RA Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S.,
RA Isogai T.;
RT "Signal sequence and keyword trap in silico for selection of full-length
RT human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA
RT libraries.";
RL DNA Res. 12:117-126(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Pancreas;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Believed to act as a modulator of nicotinic acetylcholine
CC receptors (nAChRs) activity. In vitro increases receptor
CC desensitization and decreases affinity for ACh of alpha-4:beta-2-
CC containing nAChRs. May play a role in the intracellular trafficking of
CC alpha-4:beta-2 and alpha-7-containing nAChRs and may inhibit their
CC expression at the cell surface. May be involved in the control of
CC anxiety. {ECO:0000250|UniProtKB:Q8BLC3}.
CC -!- SUBUNIT: Interacts with CHRNA4 and nAChRs containing alpha-4:beta-2
CC (CHRNA4:CHRNB2) and alpha-7 (CHRNA7) subunits.
CC {ECO:0000250|UniProtKB:Q8BLC3}.
CC -!- INTERACTION:
CC Q8N2G4-2; Q9NRY7: PLSCR2; NbExp=3; IntAct=EBI-18973558, EBI-3937430;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor, GPI-
CC anchor {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q8N2G4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8N2G4-2; Sequence=VSP_021584;
CC Name=3;
CC IsoId=Q8N2G4-3; Sequence=VSP_021583;
CC Name=4;
CC IsoId=Q8N2G4-4; Sequence=VSP_046981;
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC11647.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AY358628; AAQ88991.1; -; mRNA.
DR EMBL; AK094501; BAC04368.1; -; mRNA.
DR EMBL; AK130005; BAC85273.1; -; mRNA.
DR EMBL; AK123029; BAC85518.1; -; mRNA.
DR EMBL; AK075487; BAC11647.1; ALT_FRAME; mRNA.
DR EMBL; AC010974; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC079773; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC017318; AAH17318.1; -; mRNA.
DR CCDS; CCDS42759.1; -. [Q8N2G4-1]
DR CCDS; CCDS46416.1; -. [Q8N2G4-4]
DR RefSeq; NP_001070895.1; NM_001077427.3. [Q8N2G4-4]
DR RefSeq; NP_001308163.1; NM_001321234.1. [Q8N2G4-3]
DR RefSeq; NP_001308164.1; NM_001321235.1. [Q8N2G4-4]
DR RefSeq; NP_653187.3; NM_144586.6. [Q8N2G4-1]
DR PDB; 6ZSS; NMR; -; A=22-107.
DR PDBsum; 6ZSS; -.
DR AlphaFoldDB; Q8N2G4; -.
DR SMR; Q8N2G4; -.
DR BioGRID; 125502; 69.
DR IntAct; Q8N2G4; 20.
DR STRING; 9606.ENSP00000380605; -.
DR GlyGen; Q8N2G4; 1 site.
DR PhosphoSitePlus; Q8N2G4; -.
DR BioMuta; LYPD1; -.
DR DMDM; 92058725; -.
DR MassIVE; Q8N2G4; -.
DR PaxDb; Q8N2G4; -.
DR PeptideAtlas; Q8N2G4; -.
DR PRIDE; Q8N2G4; -.
DR ProteomicsDB; 43421; -.
DR ProteomicsDB; 71693; -. [Q8N2G4-1]
DR ProteomicsDB; 71694; -. [Q8N2G4-2]
DR ProteomicsDB; 71695; -. [Q8N2G4-3]
DR Antibodypedia; 56153; 81 antibodies from 19 providers.
DR DNASU; 116372; -.
DR Ensembl; ENST00000345008.6; ENSP00000340563.6; ENSG00000150551.11. [Q8N2G4-4]
DR Ensembl; ENST00000397463.3; ENSP00000380605.2; ENSG00000150551.11. [Q8N2G4-1]
DR GeneID; 116372; -.
DR KEGG; hsa:116372; -.
DR MANE-Select; ENST00000397463.3; ENSP00000380605.2; NM_144586.7; NP_653187.3.
DR UCSC; uc002ttn.4; human. [Q8N2G4-1]
DR CTD; 116372; -.
DR DisGeNET; 116372; -.
DR GeneCards; LYPD1; -.
DR HGNC; HGNC:28431; LYPD1.
DR HPA; ENSG00000150551; Tissue enhanced (brain, fallopian tube, pituitary gland).
DR MIM; 610450; gene.
DR neXtProt; NX_Q8N2G4; -.
DR OpenTargets; ENSG00000150551; -.
DR PharmGKB; PA134862471; -.
DR VEuPathDB; HostDB:ENSG00000150551; -.
DR eggNOG; ENOG502S22T; Eukaryota.
DR GeneTree; ENSGT00390000002215; -.
DR HOGENOM; CLU_152037_0_0_1; -.
DR InParanoid; Q8N2G4; -.
DR OMA; TFCGLFW; -.
DR OrthoDB; 1377473at2759; -.
DR PhylomeDB; Q8N2G4; -.
DR TreeFam; TF332325; -.
DR PathwayCommons; Q8N2G4; -.
DR Reactome; R-HSA-163125; Post-translational modification: synthesis of GPI-anchored proteins.
DR SignaLink; Q8N2G4; -.
DR BioGRID-ORCS; 116372; 10 hits in 1059 CRISPR screens.
DR ChiTaRS; LYPD1; human.
DR GeneWiki; LYPD1; -.
DR GenomeRNAi; 116372; -.
DR Pharos; Q8N2G4; Tbio.
DR PRO; PR:Q8N2G4; -.
DR Proteomes; UP000005640; Chromosome 2.
DR RNAct; Q8N2G4; protein.
DR Bgee; ENSG00000150551; Expressed in right uterine tube and 121 other tissues.
DR ExpressionAtlas; Q8N2G4; baseline and differential.
DR Genevisible; Q8N2G4; HS.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005576; C:extracellular region; TAS:Reactome.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0045202; C:synapse; IEA:GOC.
DR GO; GO:0033130; F:acetylcholine receptor binding; IEA:Ensembl.
DR GO; GO:0030550; F:acetylcholine receptor inhibitor activity; IBA:GO_Central.
DR GO; GO:0095500; P:acetylcholine receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0001662; P:behavioral fear response; IEA:Ensembl.
DR GO; GO:1903077; P:negative regulation of protein localization to plasma membrane; IEA:Ensembl.
DR GO; GO:0072659; P:protein localization to plasma membrane; IEA:Ensembl.
DR GO; GO:0035094; P:response to nicotine; IEA:Ensembl.
DR GO; GO:0007271; P:synaptic transmission, cholinergic; IEA:Ensembl.
DR InterPro; IPR045860; Snake_toxin-like_sf.
DR SUPFAM; SSF57302; SSF57302; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Cell membrane; Disulfide bond;
KW Glycoprotein; GPI-anchor; Lipoprotein; Membrane; Reference proteome;
KW Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..117
FT /note="Ly6/PLAUR domain-containing protein 1"
FT /id="PRO_0000226741"
FT PROPEP 118..141
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000226742"
FT DOMAIN 25..107
FT /note="UPAR/Ly6"
FT LIPID 117
FT /note="GPI-anchor amidated serine"
FT /evidence="ECO:0000255"
FT CARBOHYD 45
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 25..54
FT /evidence="ECO:0000250|UniProtKB:P0DP57,
FT ECO:0000250|UniProtKB:P0DP58"
FT DISULFID 28..37
FT /evidence="ECO:0000250|UniProtKB:P0DP57,
FT ECO:0000250|UniProtKB:P0DP58"
FT DISULFID 46..77
FT /evidence="ECO:0000250|UniProtKB:P0DP57,
FT ECO:0000250|UniProtKB:P0DP58"
FT DISULFID 88..100
FT /evidence="ECO:0000250|UniProtKB:P0DP57,
FT ECO:0000250|UniProtKB:P0DP58"
FT DISULFID 101..106
FT /evidence="ECO:0000250|UniProtKB:P0DP57,
FT ECO:0000250|UniProtKB:P0DP58"
FT VAR_SEQ 1..52
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000305"
FT /id="VSP_046981"
FT VAR_SEQ 1..5
FT /note="MWVLG -> MQAPRAAPAAPLSYDRRLRGS (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_021583"
FT VAR_SEQ 1
FT /note="M -> MQAPRAAPAAPLSYDRRPRDSGRM (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_021584"
FT CONFLICT 107
FT /note="N -> S (in Ref. 2; BAC85518)"
FT /evidence="ECO:0000305"
FT CONFLICT 133
FT /note="K -> R (in Ref. 2; BAC85518)"
FT /evidence="ECO:0000305"
FT STRAND 33..35
FT /evidence="ECO:0007829|PDB:6ZSS"
FT STRAND 42..44
FT /evidence="ECO:0007829|PDB:6ZSS"
FT STRAND 48..60
FT /evidence="ECO:0007829|PDB:6ZSS"
FT STRAND 65..73
FT /evidence="ECO:0007829|PDB:6ZSS"
FT HELIX 74..82
FT /evidence="ECO:0007829|PDB:6ZSS"
FT STRAND 97..103
FT /evidence="ECO:0007829|PDB:6ZSS"
SQ SEQUENCE 141 AA; 15240 MW; CFA6D98BA90792E3 CRC64;
MWVLGIAATF CGLFLLPGFA LQIQCYQCEE FQLNNDCSSP EFIVNCTVNV QDMCQKEVME
QSAGIMYRKS CASSAACLIA SAGYQSFCSP GKLNSVCISC CNTPLCNGPR PKKRGSSASA
LRPGLRTTIL FLKLALFSAH C
