ID   LYS1_CITFR              Reviewed;          51 AA.
AC   P06962;
DT   01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1988, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=Lysis protein for colicin A;
DE   Flags: Precursor;
GN   Name=cal;
OS   Citrobacter freundii.
OG   Plasmid ColA-CA31.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
OX   NCBI_TaxID=546;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3889552; DOI=10.1007/bf00327516;
RA   Cavard D., Lloubes R., Morlon J., Chartier M., Lazdunski C.;
RT   "Lysis protein encoded by plasmid ColA-CA31. Gene sequence and export.";
RL   Mol. Gen. Genet. 199:95-100(1985).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2832701; DOI=10.1007/bf00330599;
RA   Morlon J., Chartier M., Bidaud M., Lazdunski C.;
RT   "The complete nucleotide sequence of the colicinogenic plasmid ColA. High
RT   extent of homology with ColE1.";
RL   Mol. Gen. Genet. 211:231-243(1988).
CC   -!- FUNCTION: Lysis proteins are required for both colicin release and
CC       partial cell lysis.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000305}; Lipid-anchor
CC       {ECO:0000255|PROSITE-ProRule:PRU00303}.
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DR   EMBL; X02391; CAA26233.1; -; Genomic_DNA.
DR   EMBL; M37402; AAA72881.1; -; Genomic_DNA.
DR   PIR; S07307; S07307.
DR   RefSeq; WP_008323621.1; NZ_RZVG01000010.1.
DR   RefSeq; YP_004933727.1; NC_016151.1.
DR   AlphaFoldDB; P06962; -.
DR   SMR; P06962; -.
DR   TCDB; 1.A.73.1.2; the colicin lysis protein (clp) family.
DR   PRIDE; P06962; -.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0019835; P:cytolysis; IEA:InterPro.
DR   InterPro; IPR003059; Lysis_col.
DR   Pfam; PF02402; Lysis_col; 1.
DR   PRINTS; PR01297; LYSISCOLICIN.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Lipoprotein; Membrane; Palmitate; Plasmid; Signal.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           19..51
FT                   /note="Lysis protein for colicin A"
FT                   /id="PRO_0000005680"
FT   REGION          27..51
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   LIPID           19
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           19
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   51 AA;  5088 MW;  EB8FA4C85E38EA26 CRC64;
     MKKIIICVIL LAIMLLAACQ VNNVRDTGGG SVSPSSIVTG VSMGSDGVGN P