ID   LYS2_ECOLX              Reviewed;          47 AA.
AC   P06963;
DT   01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1988, sequence version 1.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=Lysis protein for colicins E2 and E3;
DE   Flags: Precursor;
GN   Name=hic; Synonyms=celB;
OS   Escherichia coli.
OG   Plasmid ColE2-P9, and Plasmid ColE3-CA38.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   PLASMID=ColE2-P9;
RX   PubMed=3516985; DOI=10.1093/oxfordjournals.jbchem.a135515;
RA   Toba M., Masaki H., Ohta T.;
RT   "Primary structures of the ColE2-P9 and ColE3-CA38 lysis genes.";
RL   J. Biochem. 99:591-596(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   PLASMID=ColE2-P9;
RX   PubMed=3892228; DOI=10.1007/bf00332940;
RA   Cole S.T., Saint-Joanis B., Pugsley A.P.;
RT   "Molecular characterisation of the colicin E2 operon and identification of
RT   its products.";
RL   Mol. Gen. Genet. 198:465-472(1985).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   PLASMID=ColE3-CA38;
RX   PubMed=6092219; DOI=10.1016/0378-1119(84)90178-1;
RA   Watson R.J., Lau P.C.K., Vernet T., Visentin L.P.;
RT   "Characterization and nucleotide sequence of a colicin-release gene in the
RT   hic region of plasmid ColE3-CA38.";
RL   Gene 29:175-184(1984).
RN   [4]
RP   ERRATUM OF PUBMED:6092219.
RA   Watson R.J., Lau P.C.K., Vernet T., Visentin L.P.;
RL   Gene 42:351-353(1986).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-38.
RC   PLASMID=ColE3-CA38;
RX   PubMed=3889348; DOI=10.1016/0022-2836(85)90340-7;
RA   Masaki H., Ohta T.;
RT   "Colicin E3 and its immunity genes.";
RL   J. Mol. Biol. 182:217-227(1985).
CC   -!- FUNCTION: Lysis proteins are required for both colicin release and
CC       partial cell lysis.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000305}; Lipid-anchor
CC       {ECO:0000255|PROSITE-ProRule:PRU00303}.
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DR   EMBL; X02397; CAA26243.1; -; Genomic_DNA.
DR   EMBL; D00021; BAA00015.1; -; Genomic_DNA.
DR   EMBL; J01574; AAA88419.1; -; Genomic_DNA.
DR   EMBL; X03631; CAA27281.1; -; Genomic_DNA.
DR   EMBL; X03632; CAA27282.1; -; Genomic_DNA.
DR   EMBL; M29885; AAA23070.1; -; Genomic_DNA.
DR   PIR; A91514; BVECH8.
DR   RefSeq; WP_000724383.1; NZ_VTWF01000215.1.
DR   AlphaFoldDB; P06963; -.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0019835; P:cytolysis; IEA:InterPro.
DR   InterPro; IPR003059; Lysis_col.
DR   InterPro; IPR012640; Membr_lipoprot_lipid_attach_CS.
DR   Pfam; PF08139; LPAM_1; 1.
DR   Pfam; PF02402; Lysis_col; 1.
DR   PRINTS; PR01297; LYSISCOLICIN.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Lipoprotein; Membrane; Palmitate; Plasmid; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           20..47
FT                   /note="Lysis protein for colicins E2 and E3"
FT                   /id="PRO_0000005681"
FT   LIPID           20
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           20
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   47 AA;  4860 MW;  691E149A8A164A0A CRC64;
     MKKITGIILL LLAVIILSAC QANYIRDVQG GTVSPSSTAE VTGLATQ