ID   LYS3_ECOLX              Reviewed;          45 AA.
AC   P05821;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1988, sequence version 1.
DT   25-MAY-2022, entry version 90.
DE   RecName: Full=Lysis protein for colicin E1;
DE   Flags: Precursor;
GN   Name=lys;
OS   Escherichia coli.
OG   Plasmid ColE1.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3936034; DOI=10.1073/pnas.82.24.8389;
RA   Waleh N.S., Johnson P.H.;
RT   "Structural and functional organization of the colicin E1 operon.";
RL   Proc. Natl. Acad. Sci. U.S.A. 82:8389-8393(1985).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=EC31, and EC71;
RX   PubMed=7972047; DOI=10.1073/pnas.91.23.11276;
RA   Riley M.A., Tan Y., Wang J.;
RT   "Nucleotide polymorphism in colicin E1 and Ia plasmids from natural
RT   isolates of Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 91:11276-11280(1994).
CC   -!- FUNCTION: Lysis proteins are required for both colicin release and
CC       partial cell lysis.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000305}; Lipid-anchor
CC       {ECO:0000255|PROSITE-ProRule:PRU00303}.
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DR   EMBL; M12543; AAA23067.1; -; Genomic_DNA.
DR   EMBL; U15629; AAA59411.1; -; Genomic_DNA.
DR   EMBL; U15633; AAA59419.1; -; Genomic_DNA.
DR   PIR; B24685; ZHECP1.
DR   RefSeq; WP_001405930.1; NZ_WVVR01000058.1.
DR   AlphaFoldDB; P05821; -.
DR   SMR; P05821; -.
DR   TCDB; 1.A.73.1.1; the colicin lysis protein (clp) family.
DR   GeneID; 39475527; -.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0019835; P:cytolysis; IEA:InterPro.
DR   InterPro; IPR003059; Lysis_col.
DR   Pfam; PF02402; Lysis_col; 1.
DR   PRINTS; PR01297; LYSISCOLICIN.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Lipoprotein; Membrane; Palmitate; Plasmid; Signal.
FT   SIGNAL          1..17
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           18..45
FT                   /note="Lysis protein for colicin E1"
FT                   /id="PRO_0000005682"
FT   LIPID           18
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           18
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   45 AA;  4829 MW;  A34D3B4FBC12A13E CRC64;
     MRKRFFVGIF AINLLVGCQA NYIRDVQGGT IAPSSSSKLT GIAVQ