ID   LYS4_ECOLX              Reviewed;          52 AA.
AC   P09181;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   25-MAY-2022, entry version 63.
DE   RecName: Full=Lysis protein for colicin N;
DE   Flags: Precursor;
GN   Name=cnl;
OS   Escherichia coli.
OG   Plasmid ColN pCHAP4.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], DIACYLGLYCEROL AT CYS-18, AND
RP   PALMITOYLATION AT CYS-18.
RX   PubMed=3280946; DOI=10.1007/bf00330613;
RA   Pugsley A.P.;
RT   "The immunity and lysis genes of ColN plasmid pCHAP4.";
RL   Mol. Gen. Genet. 211:335-341(1988).
CC   -!- FUNCTION: Lysis proteins are required for both colicin release and
CC       partial cell lysis.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000305}; Lipid-anchor
CC       {ECO:0000255|PROSITE-ProRule:PRU00303}.
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DR   EMBL; X06933; CAA30019.1; -; Genomic_DNA.
DR   PIR; S01760; ZHECN4.
DR   AlphaFoldDB; P09181; -.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0019835; P:cytolysis; IEA:InterPro.
DR   InterPro; IPR003059; Lysis_col.
DR   Pfam; PF02402; Lysis_col; 1.
DR   PRINTS; PR01297; LYSISCOLICIN.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   1: Evidence at protein level;
KW   Cell outer membrane; Lipoprotein; Membrane; Palmitate; Plasmid; Signal.
FT   SIGNAL          1..17
FT   CHAIN           18..52
FT                   /note="Lysis protein for colicin N"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303,
FT                   ECO:0000269|PubMed:3280946"
FT                   /id="PRO_0000005684"
FT   LIPID           18
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303,
FT                   ECO:0000269|PubMed:3280946"
FT   LIPID           18
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303,
FT                   ECO:0000269|PubMed:3280946"
SQ   SEQUENCE   52 AA;  5633 MW;  F6F1BFE9BDAF81B6 CRC64;
     MCGKILLILF FIMTLSACQV NHIRDVKGGT VAPSSSSRLT GLKLSKRSKD PL