ID   LYS5_ECOLX              Reviewed;          47 AA.
AC   P13344;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Lysis protein for colicin E5;
DE   Flags: Precursor;
GN   Name=lys;
OS   Escherichia coli.
OG   Plasmid ColE5-099.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2549375; DOI=10.1007/bf02464892;
RA   Lau P.C.K., Condie J.A.;
RT   "Nucleotide sequences from the colicin E5, E6 and E9 operons: presence of a
RT   degenerate transposon-like structure in the ColE9-J plasmid.";
RL   Mol. Gen. Genet. 217:269-277(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2561131; DOI=10.1099/00221287-135-10-2783;
RA   Curtis M.D., James R., Coddington A.;
RT   "An evolutionary relationship between the ColE5-099 and the ColE9-J
RT   plasmids revealed by nucleotide sequencing.";
RL   J. Gen. Microbiol. 135:2783-2788(1989).
CC   -!- FUNCTION: Lysis proteins are required for both colicin release and
CC       partial cell lysis.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000305}; Lipid-anchor
CC       {ECO:0000255|PROSITE-ProRule:PRU00303}.
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DR   EMBL; X15857; CAA33861.1; -; Genomic_DNA.
DR   EMBL; M30445; AAA98053.1; -; Genomic_DNA.
DR   PIR; JQ0330; JQ0330.
DR   RefSeq; WP_000724568.1; NZ_RRSE01000222.1.
DR   AlphaFoldDB; P13344; -.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0019835; P:cytolysis; IEA:InterPro.
DR   InterPro; IPR003059; Lysis_col.
DR   InterPro; IPR012640; Membr_lipoprot_lipid_attach_CS.
DR   Pfam; PF08139; LPAM_1; 1.
DR   Pfam; PF02402; Lysis_col; 1.
DR   PRINTS; PR01297; LYSISCOLICIN.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Lipoprotein; Membrane; Palmitate; Plasmid; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           20..47
FT                   /note="Lysis protein for colicin E5"
FT                   /id="PRO_0000005685"
FT   LIPID           20
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           20
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   47 AA;  4926 MW;  69133B25CA15A4B8 CRC64;
     MKKITWIILL LLAAIILAAC QANYIHDVQG GTVSPSSSAE LTGLATQ