ID   LYS8_ECOLX              Reviewed;          47 AA.
AC   P10099;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   25-MAY-2022, entry version 90.
DE   RecName: Full=Lysis protein for colicin E8;
DE   Flags: Precursor;
GN   Name=lys;
OS   Escherichia coli.
OG   Plasmid ColE8.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3323826; DOI=10.1007/bf00331154;
RA   Uchimura T., Lau P.C.K.;
RT   "Nucleotide sequences from the colicin E8 operon: homology with plasmid
RT   ColE2-P9.";
RL   Mol. Gen. Genet. 209:489-493(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3290201; DOI=10.1128/jb.170.7.3237-3242.1988;
RA   Toba M., Masaki H., Ohta T.;
RT   "Colicin E8, a DNase which indicates an evolutionary relationship between
RT   colicins E2 and E3.";
RL   J. Bacteriol. 170:3237-3242(1988).
CC   -!- FUNCTION: Lysis proteins are required for both colicin release and
CC       partial cell lysis.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000305}; Lipid-anchor
CC       {ECO:0000255|PROSITE-ProRule:PRU00303}.
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DR   EMBL; M21404; AAA23075.1; -; Genomic_DNA.
DR   EMBL; X06119; CAA29493.1; -; Genomic_DNA.
DR   PIR; C28184; ZHECE8.
DR   RefSeq; WP_001314446.1; NZ_VTVT01000225.1.
DR   RefSeq; YP_002993421.1; NC_012882.1.
DR   AlphaFoldDB; P10099; -.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0019835; P:cytolysis; IEA:InterPro.
DR   InterPro; IPR003059; Lysis_col.
DR   InterPro; IPR012640; Membr_lipoprot_lipid_attach_CS.
DR   Pfam; PF08139; LPAM_1; 1.
DR   Pfam; PF02402; Lysis_col; 1.
DR   PRINTS; PR01297; LYSISCOLICIN.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Lipoprotein; Membrane; Palmitate; Plasmid; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           20..47
FT                   /note="Lysis protein for colicin E8"
FT                   /id="PRO_0000005688"
FT   LIPID           20
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           20
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   47 AA;  4844 MW;  691E149A8A056B38 CRC64;
     MKKITGIILL LLAVIILAAC QANYIRDVQG GTVSPSSTAE VTGLATQ