ID   LYS9_ECOLX              Reviewed;          47 AA.
AC   P15176;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Lysis protein for colicin E9;
DE   Flags: Precursor;
GN   Name=lys;
OS   Escherichia coli.
OG   Plasmid ColE9-J.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Lau P.C.K.;
RL   Submitted (DEC-1989) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3312476; DOI=10.1099/00221287-133-6-1553;
RA   James R., Jarvis M., Barker D.F.;
RT   "Nucleotide sequence of the immunity and lysis region of the ColE9-J
RT   plasmid.";
RL   J. Gen. Microbiol. 133:1553-1562(1987).
CC   -!- FUNCTION: Lysis proteins are required for both colicin release and
CC       partial cell lysis.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000305}; Lipid-anchor
CC       {ECO:0000255|PROSITE-ProRule:PRU00303}.
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DR   EMBL; X15858; CAA33866.1; -; Genomic_DNA.
DR   EMBL; M16803; AAA23079.1; -; Genomic_DNA.
DR   PIR; D32535; D32535.
DR   RefSeq; WP_001426351.1; NZ_WEQK01000075.1.
DR   RefSeq; YP_002533540.1; NC_011977.1.
DR   AlphaFoldDB; P15176; -.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0019835; P:cytolysis; IEA:InterPro.
DR   InterPro; IPR003059; Lysis_col.
DR   InterPro; IPR012640; Membr_lipoprot_lipid_attach_CS.
DR   Pfam; PF08139; LPAM_1; 1.
DR   Pfam; PF02402; Lysis_col; 1.
DR   PRINTS; PR01297; LYSISCOLICIN.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Lipoprotein; Membrane; Palmitate; Plasmid; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           20..47
FT                   /note="Lysis protein for colicin E9"
FT                   /id="PRO_0000005689"
FT   LIPID           20
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           20
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   47 AA;  4816 MW;  69062F749A057ED8 CRC64;
     MKKITGIILL LLAAIILAAC QANYIRDVQG GTVSPSSSAE LTGLATQ