LYSA_DICDI
ID LYSA_DICDI Reviewed; 181 AA.
AC Q8T1G4; Q554H2;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 25-MAY-2022, entry version 74.
DE RecName: Full=Lysozyme A;
DE EC=3.2.1.n3;
DE AltName: Full=1,4-beta-N-acetylmuramidase A;
DE Flags: Precursor;
GN Name=alyA {ECO:0000312|EMBL:EAL69842.1}; ORFNames=DDB_G0275123;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [2] {ECO:0000312|EMBL:EAL69842.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=AX4 {ECO:0000269|PubMed:15010511};
RX PubMed=15010511; DOI=10.1093/nar/gkh262;
RA Urushihara H., Morio T., Saito T., Kohara Y., Koriki E., Ochiai H.,
RA Maeda M., Williams J.G., Takeuchi I., Tanaka Y.;
RT "Analyses of cDNAs from growth and slug stages of Dictyostelium
RT discoideum.";
RL Nucleic Acids Res. 32:1647-1653(2004).
RN [4] {ECO:0000305}
RP PROTEIN SEQUENCE OF 20-44, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL
RP PROPERTIES, SUBCELLULAR LOCATION, DISULFIDE BONDS, MASS SPECTROMETRY, AND
RP DISRUPTION PHENOTYPE.
RC STRAIN=AX2 {ECO:0000269|PubMed:15640146};
RX PubMed=15640146; DOI=10.1074/jbc.m411445200;
RA Mueller I., Subert N., Otto H., Herbst R., Ruehling H., Maniak M.,
RA Leippe M.;
RT "A Dictyostelium mutant with reduced lysozyme levels compensates by
RT increased phagocytic activity.";
RL J. Biol. Chem. 280:10435-10443(2005).
CC -!- FUNCTION: Has antibacterial activity against the Gram-positive bacteria
CC B.subtilis, B.megaterium and M.luteus. No antibacterial activity
CC detected against the Gram-positive bacterium S.aureus or against the
CC Gram-negative bacterium E.coli. Lacks chitinase activity.
CC {ECO:0000269|PubMed:15640146}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid
CC and N-acetyl-D-glucosamine residues in a peptidoglycan.; EC=3.2.1.n3;
CC Evidence={ECO:0000269|PubMed:15640146};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Temperature dependence:
CC Stable when incubated at 50 degrees Celsius for 10 minutes at pH 5.5.
CC Activity decreases by 50% when incubated at 60 degrees Celsius for 10
CC minutes at pH 5.5. {ECO:0000269|PubMed:15640146};
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle lumen
CC {ECO:0000269|PubMed:15640146}.
CC -!- PTM: Contains six disulfide bonds. {ECO:0000269|PubMed:15640146,
CC ECO:0000305}.
CC -!- MASS SPECTROMETRY: Mass=12652; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:15640146};
CC -!- DISRUPTION PHENOTYPE: Mutants have only 40% of the wild-type lysozyme
CC activity and initially form plaques on bacterial lawns that are only
CC half the size of wild type plaques. Despite the lysozyme activity
CC remaining constantly at 40% of wild-type, the efficiency of plaque
CC formation gradually increases over several weeks repeated plating until
CC the size of mutant plaques finally exceeds that of wild-type cells by
CC almost 2-fold. {ECO:0000269|PubMed:15640146}.
CC -!- SIMILARITY: Belongs to the dictyostelium lysozyme family.
CC {ECO:0000255}.
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DR EMBL; AAFI02000013; EAL69842.1; -; Genomic_DNA.
DR EMBL; C92846; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_643739.1; XM_638647.1.
DR AlphaFoldDB; Q8T1G4; -.
DR STRING; 44689.DDB0231275; -.
DR PaxDb; Q8T1G4; -.
DR ABCD; Q8T1G4; 9 sequenced antibodies.
DR EnsemblProtists; EAL69842; EAL69842; DDB_G0275123.
DR GeneID; 8619783; -.
DR KEGG; ddi:DDB_G0275123; -.
DR dictyBase; DDB_G0275123; alyA.
DR eggNOG; ENOG502RCK0; Eukaryota.
DR HOGENOM; CLU_1491704_0_0_1; -.
DR InParanoid; Q8T1G4; -.
DR OMA; FGCGKYL; -.
DR PhylomeDB; Q8T1G4; -.
DR PRO; PR:Q8T1G4; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0031410; C:cytoplasmic vesicle; IDA:UniProtKB.
DR GO; GO:0060205; C:cytoplasmic vesicle lumen; IEA:UniProtKB-SubCell.
DR GO; GO:0031983; C:vesicle lumen; IDA:UniProtKB.
DR GO; GO:0003796; F:lysozyme activity; IDA:UniProtKB.
DR GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR GO; GO:0009253; P:peptidoglycan catabolic process; IC:dictyBase.
DR GO; GO:0006909; P:phagocytosis; IMP:dictyBase.
PE 1: Evidence at protein level;
KW Antibiotic; Antimicrobial; Bacteriolytic enzyme; Cytoplasmic vesicle;
KW Direct protein sequencing; Disulfide bond; Glycosidase; Hydrolase;
KW Reference proteome; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000269|PubMed:15640146"
FT CHAIN 20..138
FT /note="Lysozyme A"
FT /evidence="ECO:0000269|PubMed:15640146"
FT /id="PRO_0000315914"
FT PROPEP 139..181
FT /evidence="ECO:0000269|PubMed:15640146"
FT /id="PRO_0000315915"
SQ SEQUENCE 181 AA; 19526 MW; 27B032BE137C5A3F CRC64;
MRIAFFLLVL AVIIGFAYGY SCPKPCYGNM CCSTSPNNQY YLTDFCGSTS ACGPVPSCSG
ALYFTADSQR FGCGKYLNLC RSGKCVKAQI YDAGPAMWVE QDAGMMIIDA SPTICHVLTG
GSSCGWSDHF LITATVTSLT DSRPLGPFNV TESEMAQLFI DHEIAMAQCE AEKTCNGFDL
E
