LYSO_ECOLI
ID LYSO_ECOLI Reviewed; 299 AA.
AC P75826; Q9R2W1;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 27-APR-2001, sequence version 2.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Lysine exporter LysO {ECO:0000305};
DE AltName: Full=Lys outward permease {ECO:0000303|PubMed:25845847};
GN Name=lysO {ECO:0000303|PubMed:25845847}; Synonyms=ybjE;
GN OrderedLocusNames=b0874, JW0858;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=25845847; DOI=10.1128/jb.02505-14;
RA Pathania A., Sardesai A.A.;
RT "Distinct paths for basic amino acid export in Escherichia coli: YbjE
RT (LysO) mediates export of L-lysine.";
RL J. Bacteriol. 197:2036-2047(2015).
CC -!- FUNCTION: Mediates export of lysine. {ECO:0000269|PubMed:25845847}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000255}.
CC -!- INDUCTION: Repressed by arginine, via the ArgR repressor.
CC {ECO:0000269|PubMed:25845847}.
CC -!- DISRUPTION PHENOTYPE: Null mutant is hypersensitive to the lysine
CC antimetabolite thialysine. {ECO:0000269|PubMed:25845847}.
CC -!- SIMILARITY: Belongs to the LysO family. {ECO:0000305}.
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DR EMBL; U00096; AAC73961.2; -; Genomic_DNA.
DR EMBL; AP009048; BAA35588.1; -; Genomic_DNA.
DR RefSeq; NP_415395.2; NC_000913.3.
DR RefSeq; WP_000491142.1; NZ_STEB01000019.1.
DR AlphaFoldDB; P75826; -.
DR BioGRID; 4259999; 17.
DR BioGRID; 851475; 1.
DR DIP; DIP-11447N; -.
DR IntAct; P75826; 2.
DR STRING; 511145.b0874; -.
DR TCDB; 2.A.124.1.1; the lysine exporter (lyso) family.
DR PaxDb; P75826; -.
DR PRIDE; P75826; -.
DR EnsemblBacteria; AAC73961; AAC73961; b0874.
DR EnsemblBacteria; BAA35588; BAA35588; BAA35588.
DR GeneID; 66670852; -.
DR GeneID; 947142; -.
DR KEGG; ecj:JW0858; -.
DR KEGG; eco:b0874; -.
DR PATRIC; fig|1411691.4.peg.1403; -.
DR EchoBASE; EB3200; -.
DR eggNOG; COG2431; Bacteria.
DR HOGENOM; CLU_045681_1_0_6; -.
DR InParanoid; P75826; -.
DR OMA; IFITEYK; -.
DR PhylomeDB; P75826; -.
DR BioCyc; EcoCyc:G6458-MON; -.
DR BioCyc; MetaCyc:G6458-MON; -.
DR PRO; PR:P75826; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; ISM:EcoCyc.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0015661; F:L-lysine efflux transmembrane transporter activity; IMP:EcoCyc.
DR GO; GO:1903401; P:L-lysine transmembrane transport; IMP:EcoCyc.
DR InterPro; IPR005642; LysO.
DR PANTHER; PTHR35804; PTHR35804; 1.
DR Pfam; PF03956; Lys_export; 1.
PE 2: Evidence at transcript level;
KW Amino-acid transport; Cell inner membrane; Cell membrane; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..299
FT /note="Lysine exporter LysO"
FT /id="PRO_0000168737"
FT TRANSMEM 1..21
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 31..51
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 58..78
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 109..129
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 131..151
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 169..189
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 207..227
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 277..297
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 299 AA; 32340 MW; D413F61566C34241 CRC64;
MFSGLLIILV PLIVGYLIPL RQQAALKVIN QLLSWMVYLI LFFMGISLAF LDNLASNLLA
ILHYSAVSIT VILLCNIAAL MWLERGLPWR NHHQQEKLPS RIAMALESLK LCGVVVIGFA
IGLSGLAFLQ HATEASEYTL ILLLFLVGIQ LRNNGMTLKQ IVLNRRGMIV AVVVVVSSLI
GGLINAFILD LPINTALAMA SGFGWYSLSG ILLTESFGPV IGSAAFFNDL ARELIAIMLI
PGLIRRSRST ALGLCGATSM DFTLPVLQRT GGLDMVPAAI VHGFILSLLV PILIAFFSA
