ID   LYSR_ECOLI              Reviewed;         311 AA.
AC   P03030; Q2M9Z8; Q46936;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   25-MAY-2022, entry version 157.
DE   RecName: Full=Transcriptional activator protein LysR;
GN   Name=lysR; OrderedLocusNames=b2839, JW2807;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6350602; DOI=10.1016/s0022-2836(83)80022-9;
RA   Stragier P., Patte J.-C.;
RT   "Regulation of diaminopimelate decarboxylase synthesis in Escherichia coli.
RT   III. Nucleotide sequence and regulation of the lysR gene.";
RL   J. Mol. Biol. 168:333-350(1983).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 244-311.
RC   STRAIN=K12 / JM2433;
RX   PubMed=2836407; DOI=10.1016/s0021-9258(18)68433-9;
RA   Maiden M.C.J., Jones-Mortimer M.C., Henderson P.J.F.;
RT   "The cloning, DNA sequence, and overexpression of the gene araE coding for
RT   arabinose-proton symport in Escherichia coli K12.";
RL   J. Biol. Chem. 263:8003-8010(1988).
CC   -!- FUNCTION: This protein activates the transcription of the lysA gene
CC       encoding diaminopimelate decarboxylase. LysR is also a negative
CC       regulator of its own expression.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the LysR transcriptional regulatory family.
CC       {ECO:0000305}.
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DR   EMBL; J01614; AAA83862.1; -; Genomic_DNA.
DR   EMBL; U29581; AAB40486.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC75878.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE76908.1; -; Genomic_DNA.
DR   EMBL; J03732; AAA23471.1; -; Genomic_DNA.
DR   PIR; A03565; RGECK.
DR   RefSeq; NP_417316.1; NC_000913.3.
DR   RefSeq; WP_000741825.1; NZ_LN832404.1.
DR   AlphaFoldDB; P03030; -.
DR   SMR; P03030; -.
DR   BioGRID; 4262309; 10.
DR   BioGRID; 851638; 2.
DR   DIP; DIP-10135N; -.
DR   IntAct; P03030; 6.
DR   STRING; 511145.b2839; -.
DR   PaxDb; P03030; -.
DR   PRIDE; P03030; -.
DR   EnsemblBacteria; AAC75878; AAC75878; b2839.
DR   EnsemblBacteria; BAE76908; BAE76908; BAE76908.
DR   GeneID; 947311; -.
DR   KEGG; ecj:JW2807; -.
DR   KEGG; eco:b2839; -.
DR   PATRIC; fig|1411691.4.peg.3895; -.
DR   EchoBASE; EB0546; -.
DR   eggNOG; COG0583; Bacteria.
DR   HOGENOM; CLU_039613_6_3_6; -.
DR   InParanoid; P03030; -.
DR   OMA; FHAVMTT; -.
DR   PhylomeDB; P03030; -.
DR   BioCyc; EcoCyc:PD00360; -.
DR   PRO; PR:P03030; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0009089; P:lysine biosynthetic process via diaminopimelate; IDA:EcoCyc.
DR   GO; GO:0010628; P:positive regulation of gene expression; IMP:EcoCyc.
DR   CDD; cd08456; PBP2_LysR; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR037414; LysR_PBP2.
DR   InterPro; IPR005119; LysR_subst-bd.
DR   InterPro; IPR000847; Tscrpt_reg_HTH_LysR.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF00126; HTH_1; 1.
DR   Pfam; PF03466; LysR_substrate; 1.
DR   PRINTS; PR00039; HTHLYSR.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS50931; HTH_LYSR; 1.
PE   3: Inferred from homology;
KW   Activator; Cytoplasm; DNA-binding; Reference proteome; Repressor;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..311
FT                   /note="Transcriptional activator protein LysR"
FT                   /id="PRO_0000105669"
FT   DOMAIN          1..61
FT                   /note="HTH lysR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
FT   DNA_BIND        21..40
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
SQ   SEQUENCE   311 AA;  34365 MW;  3E1913BB6A2CF8F9 CRC64;
     MAAVNLRHIE IFHAVMTAGS LTEAAHLLHT SQPTVSRELA RFEKVIGLKL FERVRGRLHP
     TVQGLRLFEE VQRSWYGLDR IVSAAESLRE FRQGELSIAC LPVFSQSFLP QLLQPFLARY
     PDVSLNIVPQ ESPLLEEWLS AQRHDLGLTE TLHTPAGTER TELLSLDEVC VLPPGHPLAV
     KKVLTPDDFQ GENYISLSRT DSYRQLLDQL FTEHQVKRRM IVETHSAASV CAMVRAGVGI
     SVVNPLTALD YAASGLVVRR FSIAVPFTVS LIRPLHRPSS ALVQAFSGHL QAGLPKLVTS
     LDAILSSATT A