LYST_DROME
ID LYST_DROME Reviewed; 3535 AA.
AC Q9W060;
DT 17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT 26-JUN-2013, sequence version 2.
DT 03-AUG-2022, entry version 175.
DE RecName: Full=Lysosomal-trafficking regulator {ECO:0000305};
DE AltName: Full=Mauve protein {ECO:0000303|PubMed:22934826, ECO:0000312|FlyBase:FBgn0265988};
GN Name=mv {ECO:0000303|PubMed:22934826, ECO:0000312|FlyBase:FBgn0265988};
GN Synonyms=CG11814 {ECO:0000312|FlyBase:FBgn0265988},
GN CG5691 {ECO:0000312|FlyBase:FBgn0265988};
GN ORFNames=CG42863 {ECO:0000312|FlyBase:FBgn0265988};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000305}
RP FUNCTION, AND MUTAGENESIS OF 2772-TRP--LYS-3535 AND 3160-TRP--LYS-3535.
RX PubMed=22934826; DOI=10.1111/tra.12005;
RA Rahman M., Haberman A., Tracy C., Ray S., Kraemer H.;
RT "Drosophila mauve mutants reveal a role of LYST homologs late in the
RT maturation of phagosomes and autophagosomes.";
RL Traffic 13:1680-1692(2012).
RN [4]
RP FUNCTION, INTERACTION WITH RAB5 AND MSPS, SUBCELLULAR LOCATION, AND
RP DEVELOPMENTAL STAGE.
RX PubMed=33725482; DOI=10.1016/j.devcel.2021.02.019;
RA Lattao R., Rangone H., Llamazares S., Glover D.M.;
RT "Mauve/LYST limits fusion of lysosome-related organelles and promotes
RT centrosomal recruitment of microtubule nucleating proteins.";
RL Dev. Cell 56:1000-1013.e6(2021).
CC -!- FUNCTION: Adapter protein that regulates intracellular membrane fusion
CC reactions (By similarity) (PubMed:33725482). Regulates the fusion of
CC lysosome-related organelles (PubMed:33725482). Promotes microtubules
CC nucleation and centrosomal recruitment of microtubule nucleating
CC proteins such as msps (PubMed:33725482). In syncytial embryos, during
CC the formation of yolk granules, suppresses vesicle fusion events with
CC lipid droplets, possibly via interaction with Rab5 (PubMed:33725482).
CC In the eye, regulates pigment granules size (PubMed:22934826). In
CC hemocytes, required for the late steps of bacteria phagocytosis
CC (PubMed:22934826). In fat body, required for autophagosome maturation
CC (PubMed:22934826). {ECO:0000250|UniProtKB:Q99698,
CC ECO:0000269|PubMed:22934826, ECO:0000269|PubMed:33725482}.
CC -!- SUBUNIT: Interacts with Rab5; the interaction is independent of GDP or
CC GTP (PubMed:33725482). Interacts with msps (PubMed:33725482).
CC {ECO:0000269|PubMed:33725482}.
CC -!- SUBCELLULAR LOCATION: Vesicle {ECO:0000269|PubMed:33725482}. Cytoplasm,
CC cytoskeleton, spindle {ECO:0000269|PubMed:33725482}. Cytoplasm,
CC cytoskeleton, spindle pole {ECO:0000269|PubMed:33725482}.
CC Note=Associated to yolk granules during vitellogenesis.
CC {ECO:0000269|PubMed:33725482}.
CC -!- DEVELOPMENTAL STAGE: Expressed in follicular epithelial cells on the
CC side facing the developing oocyte where yolk components are secreted
CC before uptake into the oocyte (at protein level) (PubMed:33725482).
CC Expressed in syncytial embryos (at protein level) (PubMed:33725482).
CC {ECO:0000269|PubMed:33725482}.
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DR EMBL; AE014296; AAF47598.2; -; Genomic_DNA.
DR RefSeq; NP_647681.2; NM_139424.2.
DR SMR; Q9W060; -.
DR IntAct; Q9W060; 9.
DR STRING; 7227.FBpp0293022; -.
DR PaxDb; Q9W060; -.
DR PRIDE; Q9W060; -.
DR EnsemblMetazoa; FBtr0304060; FBpp0293022; FBgn0265988.
DR GeneID; 38259; -.
DR KEGG; dme:Dmel_CG42863; -.
DR UCSC; CG11814-RA; d. melanogaster.
DR CTD; 104144; -.
DR FlyBase; FBgn0265988; mv.
DR VEuPathDB; VectorBase:FBgn0265988; -.
DR eggNOG; KOG1786; Eukaryota.
DR HOGENOM; CLU_000276_0_0_1; -.
DR InParanoid; Q9W060; -.
DR OMA; ANWKPNY; -.
DR OrthoDB; 101142at2759; -.
DR PhylomeDB; Q9W060; -.
DR SignaLink; Q9W060; -.
DR BioGRID-ORCS; 38259; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 38259; -.
DR PRO; PR:Q9W060; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0265988; Expressed in adult Malpighian tubule (Drosophila) and 24 other tissues.
DR ExpressionAtlas; Q9W060; baseline and differential.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005819; C:spindle; IDA:UniProtKB.
DR GO; GO:0000922; C:spindle pole; IDA:UniProtKB.
DR GO; GO:0031982; C:vesicle; IDA:UniProtKB.
DR GO; GO:0019901; F:protein kinase binding; IBA:GO_Central.
DR GO; GO:0007040; P:lysosome organization; IEA:InterPro.
DR GO; GO:0045771; P:negative regulation of autophagosome size; IMP:FlyBase.
DR GO; GO:0031339; P:negative regulation of vesicle fusion; IMP:UniProtKB.
DR GO; GO:0006909; P:phagocytosis; IMP:FlyBase.
DR GO; GO:0090063; P:positive regulation of microtubule nucleation; IMP:UniProtKB.
DR GO; GO:0008104; P:protein localization; IBA:GO_Central.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR CDD; cd06071; Beach; 1.
DR Gene3D; 1.10.1540.10; -; 1.
DR Gene3D; 2.130.10.10; -; 1.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR000409; BEACH_dom.
DR InterPro; IPR036372; BEACH_dom_sf.
DR InterPro; IPR030464; LYST.
DR InterPro; IPR023362; PH-BEACH_dom.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR13743:SF86; PTHR13743:SF86; 1.
DR Pfam; PF02138; Beach; 1.
DR SMART; SM01026; Beach; 1.
DR SMART; SM00320; WD40; 4.
DR SUPFAM; SSF50978; SSF50978; 1.
DR SUPFAM; SSF81837; SSF81837; 1.
DR PROSITE; PS50197; BEACH; 1.
DR PROSITE; PS51783; PH_BEACH; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Cytoskeleton; Phagocytosis; Protein transport;
KW Reference proteome; Repeat; Transport; WD repeat.
FT CHAIN 1..3535
FT /note="Lysosomal-trafficking regulator"
FT /evidence="ECO:0000305"
FT /id="PRO_0000450208"
FT REPEAT 689..736
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT DOMAIN 2686..2784
FT /note="BEACH-type PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01119"
FT DOMAIN 2784..3081
FT /note="BEACH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00026"
FT REPEAT 3307..3346
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 3442..3486
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 3489..3527
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REGION 412..436
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1066..1132
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1592..1613
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3254..3287
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1066..1100
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1597..1613
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3267..3287
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 2772..3535
FT /note="Missing: Results in eye color defects caused by
FT oversized pigment granules; when associated with 3160-W--K-
FT 3535."
FT /evidence="ECO:0000269|PubMed:22934826"
FT MUTAGEN 3160..3535
FT /note="Missing: Results in eye color defects caused by
FT oversized pigment granules; when associated with 2772-W--K-
FT 3535."
FT /evidence="ECO:0000269|PubMed:22934826"
SQ SEQUENCE 3535 AA; 399853 MW; 8FB99F87D3BA461F CRC64;
MAGMEVKEKE EQLLLDTLIG LWSQYLQASE LEDASVKDHW LWLLLYNLQF LDDKNLQDAW
LNSHFNLMPE ELCSYLLEQV YQIISEAKRS QESSPTHEPE QQDKCIKQLR KQHNLAQLIL
STPSDCNKIL ALRTFLTDKL GHHLLTFLLR VDIKLIASQK SLCHLCINLF PNCKWSASNE
QLIPMTSIAQ FIGSFYLNSQ SKKLRRQQQP KSQLPNHISN PIKSSFEDFR GTTRSSDELA
LLLIQLLTRC VDAEQESQLS VTVHNFALGH LCSSSEQDAS GNNDELIKFE LLQLIAHCVN
NFYVLEQQQP NVHDFNSNFG QLLYALTANR RSPLLAHGVL YIMFGTLHNL VDNGVRELNQ
IDVRNFDACF EVLQEAAKST NFSTAIFLHI YKMLLRLTDN LSSQEQHLQS LMESSASTAM
PKQQQHPRHK RQRSSQLHCL GATSLSCYFQ GKLYHLLPSL EAELQEHSVR SLLRTGSCCC
HYNVRNYATC LQLATQLSSS YQKCAYKFLH YNVLHTIFVK RNPQGCPGCE DKLKSPVFHM
DLLNIYKDNY KALLNTPAML LFLKHLKHIA YLLPYDLASG ILAEVALPIF RRYKELIESG
ESKRKISKVT PLQLPSKLEN YRALHECLSI FVMYLSDIRL VKAFYNEENI RYMQDLLIIP
ELQRGVCDLI KVGIDNIAFL GENSQEQITL SRRLIQLQLN SSDRASQLFQ ALLYKCSKHS
RAKFWLDESS TPAKLEGHKP ADILYITALQ WTLNFELLKT SQLFYNEFAK IYSIPVEMLD
DEDEDEVETP TSELLPGEKK LRHGDKNIFD ILKLNYNALG CFLMLPKATM GPTTSSTATT
PTAGICGSSS LETLVSQLPL AGSTSVSVTT AASSDYADST LDYASVIGNY DSYSRDMATP
SFLQQLLACE PDESTVLFDI RGSQSSKDNP SNSLILPSEE EGATADGIIN KLFSIVGSLF
GGSPGSGGSA NSPTRANIDA DFFCLYEPNG ECKKLLLKLF EATMAICIKG FQNEEVEKMQ
KHLRKLRAII LSHATDNWSE HGEQHARDNA VIQTLQTLLK IAELSSTHQE PTGVVNSPSG
DSQQPRPRAR SFNSGSVELP RLQTVKLPAK NSLATPPTPR RRPNSSEANA GVDADYFSTR
ASLSCAADSE LELSENEHEF YLTADEGYEA DGEIADLSES ECELNGGVLA ANESTWTPFQ
PSSRYRSHIM HQGLSQLVVQ MLAELSLLCI KQPNGWTESL TQLANRLFVI RDYLGGPLCL
LKGFAPILSC SDPRLRELQQ SILELVTHLN TPEVLQCYYS ILASKQPPVD LLMRHMHHIS
SGSLRKAQPC MELDFPIITD GKIVVTSEPL VSEQIERVRL HHQQCQTNTL FTRAACIFPV
TQTRLWQPDG LTLSLWLELR GQQMHRSSMQ FNDDETRYSG EDLTKLHLLS LGTNQAMVSI
YISNNMQLIF ELVKPNEQLA PVRVEEQIEA NADTASVISA NTQAANGGTI RQALKQTKLA
LLNSFGQMHL LNGHQASADS TGGYFESSAV QLQHLRLPRH KWMHLVFGLQ QQADSVEISI
FLDGLEQHTM RLPFRNLRQL TRVHSFQLLA LGEGQPTGRS PGSSSSSRST LDGSTPRYAL
SNVILFKRRL VDPLLMMNLT AMGPDFTEFT QCQVANWKPN YGFVSLGKLS SSNFGNHLDC
MRQLRQARVL VYTAQQPDLV MSYDASQELD MACYGQPHGH MLYGELQQHQ AQTLQAATSL
SGGLSTLLYL FARIVELTGN ASTQALALDL LLQVAHSDAQ LYTEFQRQDY LSLIGYVIKS
EKCSKDVQLL RSIVNNACSQ VLITRKGDSI NVNDNTMATL VYPRLMLTVL QRYSDWHRSG
ATHSDVLDML FRCILALTRD KHPQRDFNME QLQKCGLLGA LLNLCKVYVI ESPSPVQISP
YAAECFVQIL AVFSGSPPDS SMLDEIMKLL LLLHKPSDCY VTHDRTHFYF LLTAQQPTKE
RSLVAANLSR VTTSLRRQLH PQPQELDPER AERIKKLRRL HASTSGYRKA VNEFEEHLDQ
MADCSNLNKS ALRLLSPVEA TRWRLKFKRR HPTSANSPKR SPLKIARRRI HPHLHLGKIW
QPSGHSTPSS GQSSLPNRKT DFYDQLGIIR LQQRLLILLK DFLCLLPDSS VDVVTRHYVK
LELLLVLANQ RSCAVRQAII QLVDVLTKRL PSGELNAATK LMHPLHLANQ LTIHGCDVGM
FEACLAWING MHCSLTDIQS CDAPVRIRQR FGLPSLLAIA EGTEPQWVFK ALLRLYLQNP
DDQTCLIEAG LLQCSVKALY KIYSLRVGQS NADESVVELL ATIGERAMRS VGQINLVWDI
LNLLSFYQDK QPQLIMRSFR TAQAQLQLEW LTKFFEPNSF RVAVTNDSSL SHSELRTRVE
LLIDRCTQFF TVGVGQTTGP NYVASSQELA LFQLLVSYGI SSNQRCNNFI AWGLQPSRPR
DLRSYIVDAL WRSQQDEFQR AIICDAKMIK ALLWLSLLED MPEPIENLKP LCDALGIKEN
DSSWNLVHEL DRLDQNRNNM AAKQKTLLEK TVYRFEPLVQ QCVESSMVTT RKVAELQNAE
RKALMCHMKV YDDTYTYTKW LEIIRRMTHE GAPWHSAERA ECSWELDDTE GPSRVHTRLR
RCHLDIDRRF FMNEYRPGQG HREDLEPYVR PLDYLIASYD QQLNISLNSQ ILYNFAAKFL
PVDGEIEGEI IITDLKLYFL ATYRCRYFNV NCDIANITEI WLKRYQHQET AFEILLDTNK
SLFFSLQNAD DWKIMREVFC DKIVATPDQS KVIAITQQWR EGLLTNWEYL MTLNQISGRT
YNDLMQYPVF PWVLANYNSE VLDLREGHNF RRLGRPIAVQ VEENEKHYIS NYTYIDSTNT
TMGSLILKPY HYSSHYSNSG TVLHFLVRVP PFTSYFLRYQ DNDFDLPDRT FHALNTTWLL
ASRDSPTDVK ELIPEFFCLP EMFENFERFK FGCRQNGERV EDVSLPPWSQ RDSRLFVLIH
RQALESELVR NQIHNWIDLI FGYKQSGESA VEAINVFHPA TYAVFLDSEI SDPIEREAVK
TMVKTYGQMP RQLFKSPHPA SKPLDYSLVG KPIVSTVRGL RWGVYVGSPQ LKAPSWANIH
KIPGTEHLVS FCNTNVVYAL PGRASVMQGA EPDTYNVISW GYDDRIVRIQ PLNKPQAKPK
NLLHNGTFDD ITACGCDVNS NQLWFGHKSG RISVYKCTGG LDSQQRASAK SRQSYARGFS
LSYNSAFRKM TNKGIGGGGS ERVDEAGNLH PTSSASSVNS SSISSGGDAF QRDGADLNWL
GPTLLVRHTD EITCITLSVE FKIAVTAGRD GIAVIWDLND WSYVRTIARP AEIHQSPITH
VAISPTLGDI VTVHTLPQSC NTSTDPKPTA VATRPNSLNV ADECFEVTEE NLDDFVNVNV
NPNGKSILRL HSVNARYVQH LVHEDRILAV CYSYIKEGVG VNVIATAVEG GFVRFWSSWN
LSFVSELTTG TSPIRSICYS THQHLVVLTR ESHIQVWESE GLYGNAPKFP QIVYK
