LYST_MOUSE
ID LYST_MOUSE Reviewed; 3788 AA.
AC P97412; Q62403; Q8VBS6;
DT 15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=Lysosomal-trafficking regulator;
DE AltName: Full=Beige protein;
DE AltName: Full=CHS1 homolog;
GN Name=Lyst; Synonyms=Bg, Chs1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND DISEASE.
RX PubMed=9215680; DOI=10.1093/hmg/6.7.1091;
RA Barbosa M.D.F.S., Barrat F.J., Tchernev V.T., Nguyen Q.A., Mishra V.S.,
RA Colman S.D., Pastural E., Dufourcq-Lagelouse R., Fischer A., Holcombe R.F.,
RA Wallace M.R., Brandt S.J., De Saint Basile G., Kingsmore S.F.;
RT "Identification of mutations in two major mRNA isoforms of the Chediak-
RT Higashi syndrome gene in human and mouse.";
RL Hum. Mol. Genet. 6:1091-1098(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J;
RX PubMed=8717042; DOI=10.1038/382262a0;
RA Barbosa M.D.F.S., Nguyen Q.A., Tchernev V.T., Ashley J.A., Detter J.C.,
RA Blaydes S.M., Brandt S.J., Chotai D., Hodgman C., Solari R.C.E.S.,
RA Lovett M., Kingsmore S.F.;
RT "Identification of the homologous beige and Chediak-Higashi syndrome
RT genes.";
RL Nature 382:262-265(1996).
RN [3]
RP ERRATUM OF PUBMED:8717042.
RA Barbosa M.D.F.S., Nguyen Q.A., Tchernev V.T., Ashley J.A., Detter J.C.,
RA Blaydes S.M., Brandt S.J., Chotai D., Hodgman C., Solari R.C.E.S.,
RA Lovett M., Kingsmore S.F.;
RL Nature 385:97-97(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1428-3788 (ISOFORM 1).
RC STRAIN=C57BL/6J;
RX PubMed=8673129; DOI=10.1038/ng0796-303;
RA Perou C.M., Moore K.J., Nagle D.L., Misumi D.J., Woolf E.A., McGrail S.H.,
RA Holmgren L., Brody T.B., Dussault B.J., Monroe C.A., Duyk G.M., Pryor R.J.,
RA Li L., Justice M.J., Kaplan J.;
RT "Identification of the murine beige gene by YAC complementation and
RT positional cloning.";
RL Nat. Genet. 13:303-308(1996).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=16452087; DOI=10.1074/mcp.t500041-mcp200;
RA Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.;
RT "Comprehensive identification of phosphorylation sites in postsynaptic
RT density preparations.";
RL Mol. Cell. Proteomics 5:914-922(2006).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2203, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryonic fibroblast;
RX PubMed=19131326; DOI=10.1074/mcp.m800451-mcp200;
RA Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.;
RT "Large scale localization of protein phosphorylation by use of electron
RT capture dissociation mass spectrometry.";
RL Mol. Cell. Proteomics 8:904-912(2009).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-164; THR-165; SER-166;
RP SER-1503; SER-1504; SER-2099; SER-2118; SER-2203; SER-2207 AND SER-2254,
RP AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Kidney, Liver, Lung, Pancreas, Spleen,
RC and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [9]
RP FUNCTION, TISSUE SPECIFICITY, AND VARIANT BG 3741-ILE--GLY-3788.
RX PubMed=27881733; DOI=10.1084/jem.20141461;
RA Westphal A., Cheng W., Yu J., Grassl G., Krautkraemer M., Holst O.,
RA Foeger N., Lee K.H.;
RT "Lysosomal trafficking regulator Lyst links membrane trafficking to toll-
RT like receptor-mediated inflammatory responses.";
RL J. Exp. Med. 214:227-244(2017).
CC -!- FUNCTION: Adapter protein that regulates and/or fission of
CC intracellular vesicles such as lysosomes (By similarity). Might
CC regulate trafficking of effectors involved in exocytosis (By
CC similarity). In cytotoxic T-cells and natural killer (NK) cells, has
CC role in the regulation of size, number and exocytosis of lytic granules
CC (By similarity). In macrophages and dendritic cells, regulates
CC phagosome maturation by controlling the conversion of early phagosomal
CC compartments into late phagosomes (PubMed:27881733). In macrophages and
CC dendritic cells, specifically involved in TLR3- and TLR4-induced
CC production of pro-inflammatory cytokines by regulating the endosomal
CC TLR3- TICAM1/TRIF and TLR4- TICAM1/TRIF signaling pathways
CC (PubMed:27881733). {ECO:0000250|UniProtKB:Q99698,
CC ECO:0000269|PubMed:27881733}.
CC -!- SUBUNIT: Interacts with CENPJ, LIP8 and ZNF521.
CC {ECO:0000250|UniProtKB:Q99698}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Comment=Additional isoforms seem to exist.;
CC Name=1;
CC IsoId=P97412-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P97412-2; Sequence=VSP_006783, VSP_006784;
CC -!- TISSUE SPECIFICITY: Expressed in the heart, lung, liver, spleen, brain
CC and in different immune cell types (purified B and T lymphocytes, bone
CC marrow-derived macrophages and dendritic cells).
CC {ECO:0000269|PubMed:27881733}.
CC -!- DISEASE: Note=Defects in Lyst are the cause of beige (bg), an autosomal
CC recessive disorder characterized by hypopigmentation, bleeding, immune
CC cell dysfunction, abnormal intracellular transport to and from the
CC lysosome, and giant inclusion bodies in a variety of cell types.
CC {ECO:0000269|PubMed:27881733, ECO:0000269|PubMed:9215680}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U70015; AAC53011.1; -; mRNA.
DR EMBL; L77884; AAL40134.1; -; mRNA.
DR EMBL; U52461; AAB60778.1; -; mRNA.
DR PIR; T30851; T30851.
DR RefSeq; NP_034878.2; NM_010748.2.
DR SMR; P97412; -.
DR BioGRID; 201257; 1.
DR STRING; 10090.ENSMUSP00000106188; -.
DR iPTMnet; P97412; -.
DR PhosphoSitePlus; P97412; -.
DR SwissPalm; P97412; -.
DR EPD; P97412; -.
DR jPOST; P97412; -.
DR MaxQB; P97412; -.
DR PaxDb; P97412; -.
DR PeptideAtlas; P97412; -.
DR PRIDE; P97412; -.
DR ProteomicsDB; 290203; -. [P97412-1]
DR ProteomicsDB; 290204; -. [P97412-2]
DR DNASU; 17101; -.
DR GeneID; 17101; -.
DR KEGG; mmu:17101; -.
DR CTD; 1130; -.
DR MGI; MGI:107448; Lyst.
DR eggNOG; KOG1786; Eukaryota.
DR InParanoid; P97412; -.
DR OrthoDB; 101142at2759; -.
DR PhylomeDB; P97412; -.
DR BioGRID-ORCS; 17101; 4 hits in 74 CRISPR screens.
DR ChiTaRS; Lyst; mouse.
DR PRO; PR:P97412; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; P97412; protein.
DR GO; GO:0005829; C:cytosol; IDA:MGI.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0015630; C:microtubule cytoskeleton; ISO:MGI.
DR GO; GO:0019901; F:protein kinase binding; IBA:GO_Central.
DR GO; GO:0007596; P:blood coagulation; IMP:MGI.
DR GO; GO:0042742; P:defense response to bacterium; IMP:MGI.
DR GO; GO:0042832; P:defense response to protozoan; IMP:MGI.
DR GO; GO:0051607; P:defense response to virus; IMP:MGI.
DR GO; GO:0032510; P:endosome to lysosome transport via multivesicular body sorting pathway; ISO:MGI.
DR GO; GO:0071621; P:granulocyte chemotaxis; IMP:MGI.
DR GO; GO:0007040; P:lysosome organization; IMP:MGI.
DR GO; GO:0033364; P:mast cell secretory granule organization; IMP:MGI.
DR GO; GO:0032438; P:melanosome organization; IMP:MGI.
DR GO; GO:0007017; P:microtubule-based process; IMP:MGI.
DR GO; GO:0042267; P:natural killer cell mediated cytotoxicity; ISO:MGI.
DR GO; GO:0002446; P:neutrophil mediated immunity; IMP:MGI.
DR GO; GO:0006909; P:phagocytosis; IEA:UniProtKB-KW.
DR GO; GO:0055091; P:phospholipid homeostasis; IMP:MGI.
DR GO; GO:0006644; P:phospholipid metabolic process; IMP:MGI.
DR GO; GO:0048753; P:pigment granule organization; IMP:MGI.
DR GO; GO:0043473; P:pigmentation; IMP:MGI.
DR GO; GO:0032816; P:positive regulation of natural killer cell activation; IMP:MGI.
DR GO; GO:0008104; P:protein localization; IBA:GO_Central.
DR GO; GO:0009410; P:response to xenobiotic stimulus; IMP:MGI.
DR GO; GO:0033299; P:secretion of lysosomal enzymes; IMP:MGI.
DR GO; GO:0002456; P:T cell mediated immunity; IMP:MGI.
DR CDD; cd06071; Beach; 1.
DR CDD; cd01201; PH_BEACH; 1.
DR Gene3D; 1.10.1540.10; -; 1.
DR Gene3D; 2.130.10.10; -; 2.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000409; BEACH_dom.
DR InterPro; IPR036372; BEACH_dom_sf.
DR InterPro; IPR030464; LYST.
DR InterPro; IPR023362; PH-BEACH_dom.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR13743:SF86; PTHR13743:SF86; 1.
DR Pfam; PF02138; Beach; 1.
DR Pfam; PF14844; PH_BEACH; 1.
DR Pfam; PF00400; WD40; 1.
DR SMART; SM01026; Beach; 1.
DR SMART; SM00320; WD40; 4.
DR SUPFAM; SSF48371; SSF48371; 1.
DR SUPFAM; SSF50978; SSF50978; 1.
DR SUPFAM; SSF81837; SSF81837; 1.
DR PROSITE; PS50197; BEACH; 1.
DR PROSITE; PS51783; PH_BEACH; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Disease variant; Phagocytosis;
KW Phosphoprotein; Protein transport; Reference proteome; Repeat; Transport;
KW WD repeat.
FT CHAIN 1..3788
FT /note="Lysosomal-trafficking regulator"
FT /id="PRO_0000051072"
FT REPEAT 662..700
FT /note="WD 1"
FT REPEAT 1576..1620
FT /note="WD 2"
FT DOMAIN 2996..3102
FT /note="BEACH-type PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01119"
FT DOMAIN 3126..3409
FT /note="BEACH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00026"
FT REPEAT 3550..3589
FT /note="WD 3"
FT REPEAT 3601..3640
FT /note="WD 4"
FT REPEAT 3643..3686
FT /note="WD 5"
FT REPEAT 3687..3731
FT /note="WD 6"
FT REPEAT 3736..3775
FT /note="WD 7"
FT REGION 148..180
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 198..217
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1169..1196
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1213..1240
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1482..1519
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2177..2221
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2556..2581
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2659..2681
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 159..173
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1214..1233
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1483..1518
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2187..2217
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 164
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 165
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 166
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 1503
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 1504
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 2099
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 2118
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 2203
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 2207
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 2254
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 1509..1545
FT /note="EGDRPEVTESINPGDRLIEDGCIHLISLGSKALMIQV -> GMMAGSDLYTK
FT ILQIAACLSFKHIWQYFNVFFKCYSP (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:8717042"
FT /id="VSP_006783"
FT VAR_SEQ 1546..3788
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:8717042"
FT /id="VSP_006784"
FT VARIANT 3741..3788
FT /note="Missing (in bg; macrophages and dendritic cells show
FT impaired pro-inflammatory cytokine production in response
FT to stimulation of TLR3 and TLR4 receptors; shows impaired
FT inflammatory responses to lipopolysaccharides; following
FT bacteria infections, shows defective phagosomal
FT maturation)"
FT /evidence="ECO:0000269|PubMed:27881733"
SQ SEQUENCE 3788 AA; 425287 MW; F01BE837C676A750 CRC64;
MSTDSNSLAR EFLIDVNQLC NAVVQRAEAR EEEEEETHMA TLGQYLVHGR GFLLLTKLNS
IIDQALTCRE ELLTLLLSLL PLVWKIPVQE QQATDFNLPL SSDIILTKEK NSSLQKSTQG
KLYLEGSAPS GQVSAKVNLF RKIRRQRKST HRYSVRDARK TQLSTSDSEG NSDEKSTVVS
KHRRLHALPR FLTQSPKEGH LVAKPDPSAT KEQVLSDTMS VENSREVILR QDSNGDILSE
PAALSILSNM NNSPFDLCHV LLSLLEKVCK FDIALNHNSS LALSVVPTLT EFLAGFGDCC
NQSDTLEGQL VSAGWTEEPV ALVQRMLFRT VLHLMSVDVS TAEAMPESLR KNLTELLRAA
LKIRACLEKQ PEPFSPRQKK TLQEVQEGFV FSKYRHRALL LPELLEGVLQ LLISCLQSAA
SNPFYFSQAM DLVQEFIQHQ GFNLFGTAVL QMEWLLTRDG VPSEAAEHLK ALINSVIKIM
STVKKVKSEQ LHHSMCTRKR HRRCEYSHFM QHHRDLSGLL VSAFKNQLSK SPFEETAEGD
VQYPERCCCI AVCAHQCLRL LQQVSLSTTC VQILSGVHSV GICCCMDPKS VIAPLLHAFK
LPALKAFQQH ILNVLSKLLV DQLGGAELSP RIKKAACNIC TVDSDQLAKL GETLQGTLCG
AGPTSGLPSP SYRFQGILPS SGSEDLLWKW DALEAYQSFV FQEDRLHNIQ IANHICNLLQ
KGNVVVQWKL YNYIFNPVLQ RGVELVHHCQ QLSIPSAQTH MCSQLKQYLP QEVLQIYLKT
LPVLLKSRVI RDLFLSCNGV NHIIELNYLD GIRSHSLKAF ETLIVSLGEQ QKDAAVLDVD
GLDIQQELPS LSVGPSLHKQ QASSDSPCSL RKFYASLREP DPKKRKTIHQ DVHINTINLF
LCVAFLCVSK EADSDRESAN ESEDTSGYDS PPSEPLSHML PCLSLEDVVL PSPECLHHAA
DIWSMCRWIY MLNSVFQKQF HRLGGFQVCH ELIFMIIQKL FRSHTEDQGR RQGEMSRNEN
QELIRISYPE LTLKGDVSSA TAPDLGFLRK SADSVRGFQS QPVLPTSAEQ IVATESVPGE
RKAFMSQQSE TSLQSIRLLE SLLDICLHSA RACQQKMELE LPSQGLSVEN ILCELREHLS
QSKVAETELA KPLFDALLRV ALGNHSADLG PGDAVTEKSH PSEEELLSQP GDFSEEAEDS
QCCSLKLLGE EEGYEADSES NPEDVDTQDD GVELNPEAEG FSGSIVSNNL LENLTHGEII
YPEICMLGLN LLSASKAKLD VLAHVFESFL KIVRQKEKNI SLLIQQGTVK ILLGGFLNIL
TQTNSDFQAC QRVLVDLLVS LMSSRTCSED LTLLWRIFLE KSPCTEILLL GIHKIVESDF
TMSPSQCLTF PFLHTPSLSN GVLSQKPPGI LNSKALGLLR RARISRGKKE ADRESFPYRL
LSSWHIAPIH LPLLGQNCWP HLSEGFSVSL VGLMWNTSNE SESAAERGKR VKKRNKPSVL
EDSSFEGAEG DRPEVTESIN PGDRLIEDGC IHLISLGSKA LMIQVWADPH SGTFIFRVCM
DSNDDTKAVS LAQVESQENI FFPSKWQHLV LTYIQHPQGK KNVHGEISIW VSGQRKTDVI
LDFVLPRKTS LSSDSNKTFC MIGHCLTSQE ESLQLAGKWD LGNLLLFNGA KIGSQEAFFL
YACGPNYTSI MPCKYGQPVI DYSKYINKDI LRCDEIRDLF MTKKEVDVGL LIESLSVVYT
TCCPAQYTIY EPVIRLKGQV KTQPSQRPFS SKEAQSILLE PSQLKGLQPT ECKAIQGILH
EIGGAGTFVF LFARVVELSS CEETQALALR VILSLIKYSQ QRTQELENCN GLSMIHQVLV
KQKCIVGFHI LKTLLEGCCG EEVIHVSEHG EFKLDVESHA IIQDVKLLQE LLLDWKIWNK
AEQGVWETLL AALEVLIRVE HHQQQFNIKQ LLNAHVVHHF LLTCQVLQEH REGQLTSMPR
EVCRSFVKII AEVLGSPPDL ELLTVIFNFL LAVHPPTNTY VCHNPTNFYF SLHIDGKIFQ
EKVQSLAYLR HSSSGGQAFP SPGFLVISPS AFTAAPPEGT SSSNIVPQRM AAQMVRSRSL
PAFPTYLPLI RAQKLAASLG FSVDKLQNIA DANPEKQNLL GRPYALKTSK EEAFISSCES
AKTVCEMEAL LGAHASANGV SRGSPRFPRA RVDHKDVGTE PRSDDDSPGD ESYPRRPDNL
KGLASFQRSQ STVASLGLAF PSQNGSAVAS RWPSLVDRNA DDWENFTFSP AYEASYNRAT
STHSVIEDCL IPICCGLYEL LSGVLLVLPD AMLEDVMDRI IQADILLVLV NHPSPAIQQG
VIKLLHAYIN RASKEQKDKF LKNRGFSLLA NQLYLHRGTQ ELLECFVEMF FGRPIGLDEE
FDLEEVKHME LFQKWSVIPV LGLIETSLYD NVLLHNALLL LLQVLNSCSK VADMLLDNGL
LYVLCNTVAA LNGLEKNIPV NEYKLLACDI QQLFIAVTIH ACSSSGTQYF RVIEDLIVLL
GYLHNSKNKR TQNMALALQL RVLQAALEFI RSTANHDSES PVHSPSAHRH SVPPKRRSIA
GSRKFPLAQT ESLLMKMRSV ASDELHSMMQ RRMSQEHPSQ ASEAELAQRL QRLTILAVNR
IIYQELNSDI IDILRTPENT SQSKTSVSQT EISEEDMHHE QPSVYNPFQK EMLTYLLDGF
KVCIGSSKTS VSKQQWTKIL GSCKETLRDQ LGRLLAHILS PTHTVQERKQ ILEIVHEPAH
QDILRDCLSP SPQHGAKLVL YLSELIHNHQ DELSEEEMDT AELLMNALKL CGHKCIPPSA
PSKPELIKII REEQKKYESE ESVSKGSWQK TVNNNQQSLF QRLDFKSKDI SKIAADITQA
VSLSQGIERK KVIQHIRGMY KVDLSASRHW QECIQQLTHD RAVWYDPIYY PTSWQLDPTE
GPNRERRRLQ RCYLTIPNKY LLRDRQKSEG VLRPPLSYLF EDKTHSSFSS TVKDKAASES
IRVNRRCISV APSRETAGEL LLGKCGMYFV EDNASDAVES SSLQGELEPA SFSWTYEEIK
EVHRRWWQLR DNAVEIFLTN GRTLLLAFDN NKVRDDVYQS ILTNNLPNLL EYGNITALTN
LWYSGQITNF EYLTHLNKHA GRSFNDLMQY PVFPFILSDY VSETLDLNDP SIYRNLSKPI
AVQYKEKEDR YVDTYKYLEE EYRKGAREDD PMPPVQPYHY GSHYSNSGTV LHFLVRMPPF
TKMFLAYQDQ SFDIPDRTFH STNTTWRLSS FESMTDVKEL IPEFFYLPEF LVNREGFDFG
VRQNGERVNH VNLPPWARND PRLFILIHRQ ALESDHVSQN ICHWIDLVFG YKQKGKASVQ
AINVFHPATY FGMDVSAVED PVQRRALETM IKTYGQTPRQ LFHTAHASRP GAKLNIEGEL
PAAVGLLVQF AFRETREPVK EVTHPSPLSW IKGLKWGEYV GSPSAPVPVV CFSQPHGERF
GSLQALPTRA ICGLSRNFCL LMTYNKEQGV RSMNNTNIQW SAILSWGYAD NILRLKSKQS
EPPINFIQSS QQHQVTSCAW VPDSCQLFTG SKCGVITAYT NRLTSSTPSE IEMESQMHLY
GHTEEITGLC VCKPYSVMIS VSRDGTCIVW DLNRLCYVQS LAGHKSPVTA VSASETSGDI
ATVCDSAGGG SDLRLWTVNG DLVGHVHCRE IICSVAFSNQ PEGVSINVIA GGLENGIVRL
WSTWDLKPVR EITFPKSNKP IISLTFSCDG HHLYTANSEG TVIAWCRKDQ QRVKLPMFYS
FLSSYAAG
