ID   LYS_BPS13               Reviewed;          91 AA.
AC   P07930;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=Lysis protein;
DE            Short=E protein;
DE   AltName: Full=GPE;
GN   Name=E;
OS   Enterobacteria phage S13 (Bacteriophage S13).
OC   Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes;
OC   Petitvirales; Microviridae; Bullavirinae; Sinsheimervirus;
OC   unclassified Sinsheimervirus.
OX   NCBI_TaxID=10844;
OH   NCBI_TaxID=590; Salmonella.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3007293; DOI=10.1016/0378-1119(85)90050-2;
RA   Lau P.C.K., Spencer J.H.;
RT   "Nucleotide sequence and genome organization of bacteriophage S13 DNA.";
RL   Gene 40:273-284(1985).
CC   -!- FUNCTION: Induces host cell lysis. Inhibits the host translocase MraY
CC       activity that catalyzes the synthesis of lipid I, a necessary step for
CC       the host cell wall biosynthesis. {ECO:0000250|UniProtKB:P03639}.
CC   -!- SUBUNIT: Oligomerizes. Interacts (via N-terminal) with host SlyD; this
CC       interaction supposely protects E from proteolysis and therefore allows
CC       E to interact with and inhibit host MraY at the host membrane.
CC       Interacts with host MraY; this interaction inhibits MraY translocase
CC       activity. {ECO:0000250|UniProtKB:P03639}.
CC   -!- SUBCELLULAR LOCATION: Host cell membrane {ECO:0000255}; Single-pass
CC       type I membrane protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the microvirus E protein family. {ECO:0000305}.
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DR   EMBL; M14428; AAA32588.1; -; Genomic_DNA.
DR   PIR; JS0455; JS0455.
DR   Proteomes; UP000002129; Genome.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004857; F:enzyme inhibitor activity; IEA:InterPro.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0019054; P:modulation by virus of host cellular process; IEA:InterPro.
DR   InterPro; IPR007605; Micrvir_lysisE.
DR   Pfam; PF04517; Microvir_lysis; 1.
PE   3: Inferred from homology;
KW   Cytolysis; Host cell lysis by virus; Host cell membrane; Host membrane;
KW   Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW   Viral release from host cell.
FT   CHAIN           1..91
FT                   /note="Lysis protein"
FT                   /id="PRO_0000164886"
FT   TOPO_DOM        1..8
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        9..29
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        30..91
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   91 AA;  10574 MW;  F44DB400197D829A CRC64;
     MVRWTLWDTL AFLLLLSLLL PSLLIMFIPS TFKRPVSSWK ALNLRKTLLM ASSVRLKPLN
     CSRLPCVYAQ ETLTFLLTQK KTCVKNYVQK E