LYT1_HOGCA
ID LYT1_HOGCA Reviewed; 25 AA.
AC P61507;
DT 24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2004, sequence version 1.
DT 03-AUG-2022, entry version 49.
DE RecName: Full=M-lycotoxin-Hc1a;
DE Short=M-LCTX-Hc1a;
DE AltName: Full=Lycotoxin I {ECO:0000303|PubMed:18098329};
DE AltName: Full=Lycotoxin-1;
OS Hogna carolinensis (Carolina wolf spider) (Lycosa carolinensis).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC Araneomorphae; Entelegynae; Lycosoidea; Lycosidae; Hogna.
OX NCBI_TaxID=278031;
RN [1]
RP PROTEIN SEQUENCE, FUNCTION, SYNTHESIS, MASS SPECTROMETRY, SUBCELLULAR
RP LOCATION, AND AMIDATION AT LEU-25.
RC TISSUE=Venom;
RX PubMed=9442044; DOI=10.1074/jbc.273.4.2059;
RA Yan L., Adams M.E.;
RT "Lycotoxins, antimicrobial peptides from venom of the wolf spider Lycosa
RT carolinensis.";
RL J. Biol. Chem. 273:2059-2066(1998).
RN [2]
RP FUNCTION, CIRCULAR DICHROISM, AND MEMBRANE INTERACTION OF SHORT LYCOTOXIN-1
RP ANALOG.
RX PubMed=18098329; DOI=10.1002/psc.993;
RA Adao R., Seixas R., Gomes P., Pessoa J.C., Bastos M.;
RT "Membrane structure and interactions of a short Lycotoxin I analogue.";
RL J. Pept. Sci. 14:528-534(2008).
RN [3]
RP MUTAGENESIS OF PHE-8; LEU-9; GLY-10; GLN-20; GLN-21 AND 24-LYS-LEU-25.
RX PubMed=18465835; DOI=10.1002/psc.1040;
RA Hughes S.R., Dowd P.F., Hector R.E., Panavas T., Sterner D.E., Qureshi N.,
RA Bischoff K.M., Bang S.S., Mertens J.A., Johnson E.T., Li X.-L.,
RA Jackson J.S., Caughey R.J., Riedmuller S.B., Bartolett S., Liu S.,
RA Rich J.O., Farrelly P.J., Butt T.R., Labaer J., Cotta M.A.;
RT "Lycotoxin-1 insecticidal peptide optimized by amino acid scanning
RT mutagenesis and expressed as a coproduct in an ethanologenic Saccharomyces
RT cerevisiae strain.";
RL J. Pept. Sci. 14:1039-1050(2008).
CC -!- FUNCTION: Forms pore that permeabilize the cell membrane. Promotes
CC efflux of calcium from synaptosomes, causes hemolysis, and dissipates
CC voltage gradients across muscle membrane. Potently inhibits the growth
CC of bacteria, yeast and Leishmania. Is lethal to lepidopteran larvae.
CC May function both in the prey capture strategy as well as protection
CC from infectious organisms arising from prey ingestion.
CC {ECO:0000269|PubMed:18098329, ECO:0000269|PubMed:9442044}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:9442044}. Target
CC cell membrane {ECO:0000269|PubMed:18098329}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:9442044}.
CC -!- MASS SPECTROMETRY: Mass=2843.4; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:9442044};
CC -!- MISCELLANEOUS: Mutated toxins are expressed as transgene in yeasts.
CC Yeasts containing transgenes cause lethality when feed to lepidopteran
CC larvae (S.frugiperda). {ECO:0000269|PubMed:18465835}.
CC -!- SIMILARITY: Belongs to the cationic peptide 04 (cupiennin) family. 05
CC subfamily. {ECO:0000305}.
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DR AlphaFoldDB; P61507; -.
DR SMR; P61507; -.
DR TCDB; 8.B.10.2.1; the psalmotoxin-1 (pctx1) family.
DR ArachnoServer; AS000065; M-lycotoxin-Hc1a.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Amidation; Antibiotic; Antimicrobial; Cytolysis; Direct protein sequencing;
KW Hemolysis; Ion transport; Membrane; Neurotoxin; Secreted;
KW Target cell membrane; Target membrane; Toxin; Transmembrane; Transport.
FT PEPTIDE 1..25
FT /note="M-lycotoxin-Hc1a"
FT /evidence="ECO:0000269|PubMed:9442044"
FT /id="PRO_0000044558"
FT MOD_RES 25
FT /note="Leucine amide"
FT /evidence="ECO:0000269|PubMed:9442044"
FT MUTAGEN 8
FT /note="F->H: In A6; 13% increase in lepidopteran larvae
FT mortality at day 1, and 74% at day 2."
FT /evidence="ECO:0000269|PubMed:18465835"
FT MUTAGEN 9
FT /note="L->S: In C6; 4% increase in lepidopteran larvae
FT mortality at day 1, and 100% at day 2."
FT /evidence="ECO:0000269|PubMed:18465835"
FT MUTAGEN 10
FT /note="G->Q: In B9; No increase in lepidopteran larvae
FT mortality at day 1, and 97% at day 2."
FT /evidence="ECO:0000269|PubMed:18465835"
FT MUTAGEN 20
FT /note="Q->H: In B9; No increase in lepidopteran larvae
FT mortality at day 1, and 97% at day 2."
FT /evidence="ECO:0000269|PubMed:18465835"
FT MUTAGEN 21
FT /note="Q->S: In B9; No increase in lepidopteran larvae
FT mortality at day 1, and 97% at day 2."
FT /evidence="ECO:0000269|PubMed:18465835"
FT MUTAGEN 24..25
FT /note="KL->PW: 3% increase in lepidopteran larvae mortality
FT at day 1, and 71% at day 2. In A6; 13% increase in
FT lepidopteran larvae mortality at day 1, and 74% at day 2.
FT In B9; No increase in lepidopteran larvae mortality at day
FT 1, and 97% at day 2. In C6; 4% increase in lepidopteran
FT larvae mortality at day 1, and 100% at day 2."
FT /evidence="ECO:0000269|PubMed:18465835"
SQ SEQUENCE 25 AA; 2844 MW; 0BFFF1756EB3EA09 CRC64;
IWLTALKFLG KHAAKHLAKQ QLSKL
