ID   LYTH_STAA8              Reviewed;         291 AA.
AC   Q2FXU3;
DT   11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Probable cell wall amidase LytH {ECO:0000250|UniProtKB:O32421};
DE            EC=3.5.1.- {ECO:0000250|UniProtKB:O32421};
DE   Flags: Precursor;
GN   Name=lytH; OrderedLocusNames=SAOUHSC_01739;
OS   Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCTC 8325 / PS 47;
RA   Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT   "The Staphylococcus aureus NCTC 8325 genome.";
RL   (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL   Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL   D.C. (2006).
RN   [2]
RP   PRELIMINARY FUNCTION.
RC   STRAIN=ATCC 35556 / SA113;
RX   PubMed=25690309; DOI=10.1007/s00253-015-6443-2;
RA   Osipovitch D.C., Therrien S., Griswold K.E.;
RT   "Discovery of novel S. aureus autolysins and molecular engineering to
RT   enhance bacteriolytic activity.";
RL   Appl. Microbiol. Biotechnol. 99:6315-6326(2015).
CC   -!- FUNCTION: Probably involved in cell-wall metabolism (By similarity).
CC       May have autolysin activity (PubMed:25690309).
CC       {ECO:0000250|UniProtKB:O32421, ECO:0000305|PubMed:25690309}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family.
CC       {ECO:0000305}.
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DR   EMBL; CP000253; ABD30811.1; -; Genomic_DNA.
DR   RefSeq; WP_000717800.1; NZ_LS483365.1.
DR   RefSeq; YP_500247.1; NC_007795.1.
DR   AlphaFoldDB; Q2FXU3; -.
DR   SMR; Q2FXU3; -.
DR   STRING; 1280.SAXN108_1659; -.
DR   EnsemblBacteria; ABD30811; ABD30811; SAOUHSC_01739.
DR   GeneID; 3921088; -.
DR   KEGG; sao:SAOUHSC_01739; -.
DR   PATRIC; fig|93061.5.peg.1585; -.
DR   eggNOG; COG0860; Bacteria.
DR   HOGENOM; CLU_014322_1_1_9; -.
DR   OMA; WHTNLNI; -.
DR   PRO; PR:Q2FXU3; -.
DR   Proteomes; UP000008816; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030288; C:outer membrane-bounded periplasmic space; IBA:GO_Central.
DR   GO; GO:0008745; F:N-acetylmuramoyl-L-alanine amidase activity; IBA:GO_Central.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0009253; P:peptidoglycan catabolic process; IEA:InterPro.
DR   CDD; cd02696; MurNAc-LAA; 1.
DR   InterPro; IPR017273; LytH.
DR   InterPro; IPR002508; MurNAc-LAA_cat.
DR   InterPro; IPR003646; SH3-like_bac-type.
DR   Pfam; PF01520; Amidase_3; 1.
DR   Pfam; PF08239; SH3_3; 1.
DR   PIRSF; PIRSF037730; CWA_LytH_prd; 1.
DR   SMART; SM00646; Ami_3; 1.
DR   SMART; SM00287; SH3b; 1.
DR   PROSITE; PS51781; SH3B; 1.
PE   3: Inferred from homology;
KW   Cell wall biogenesis/degradation; Hydrolase; Reference proteome; Secreted;
KW   Signal.
FT   SIGNAL          1..40
FT                   /evidence="ECO:0000255"
FT   CHAIN           41..291
FT                   /note="Probable cell wall amidase LytH"
FT                   /id="PRO_0000245426"
FT   DOMAIN          41..105
FT                   /note="SH3b"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01117"
FT   DOMAIN          122..286
FT                   /note="MurNAc-LAA"
FT                   /evidence="ECO:0000305|PubMed:25690309"
FT   REGION          118..140
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   291 AA;  32694 MW;  C296BB1178D37E1B CRC64;
     MKKIEAWLSK KGLKNKRTLI VVIAFVLFII FLFLLLNSNS EDSGNITITE NAELRTGPNA
     AYPVIYKVEK GDHFKKIGKV GKWIEVEDTS SNEKGWIAGW HTNLDIVADN TKEKNPLQGK
     TIVLDPGHGG SDQGASSNTK YKSLEKDYTL KTAKELQRTL EKEGATVKMT RTDDTYVSLE
     NRDIKGDAYL SIHNDALESS NANGMTVYWY HDNQRALADT LDATIQKKGL LSNRGSRQEN
     YQVLRQTKVP AVLLELGYIS NPTDETMIKD QLHRQILEQA IVDGLKIYFS A