ID   LYTS_BACAN              Reviewed;         589 AA.
AC   Q81JL2; Q6HQ55; Q6KJJ7;
DT   15-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 134.
DE   RecName: Full=Sensor protein LytS;
DE            EC=2.7.13.3;
GN   Name=lytS; OrderedLocusNames=BA_5692, GBAA_5692, BAS5296;
OS   Bacillus anthracis.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=1392;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Ames / isolate Porton;
RX   PubMed=12721629; DOI=10.1038/nature01586;
RA   Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T.,
RA   Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R.,
RA   Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M.,
RA   Kolonay J.F., Beanan M.J., Dodson R.J., Brinkac L.M., Gwinn M.L.,
RA   DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C.,
RA   Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y.,
RA   Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M.,
RA   Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E.,
RA   White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M.,
RA   Hanna P.C., Kolstoe A.-B., Fraser C.M.;
RT   "The genome sequence of Bacillus anthracis Ames and comparison to closely
RT   related bacteria.";
RL   Nature 423:81-86(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Ames ancestor;
RX   PubMed=18952800; DOI=10.1128/jb.01347-08;
RA   Ravel J., Jiang L., Stanley S.T., Wilson M.R., Decker R.S., Read T.D.,
RA   Worsham P., Keim P.S., Salzberg S.L., Fraser-Liggett C.M., Rasko D.A.;
RT   "The complete genome sequence of Bacillus anthracis Ames 'Ancestor'.";
RL   J. Bacteriol. 191:445-446(2009).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Sterne;
RA   Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K.,
RA   Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R.,
RA   Richardson P., Rubin E., Tice H.;
RT   "Complete genome sequence of Bacillus anthracis Sterne.";
RL   Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Member of the two-component regulatory system LytS/LytT that
CC       probably regulates genes involved in cell wall metabolism.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC         histidine.; EC=2.7.13.3;
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
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DR   EMBL; AE016879; AAP29324.1; -; Genomic_DNA.
DR   EMBL; AE017334; AAT34850.1; -; Genomic_DNA.
DR   EMBL; AE017225; AAT57583.1; -; Genomic_DNA.
DR   RefSeq; NP_847838.1; NC_003997.3.
DR   RefSeq; WP_000933570.1; NZ_WXXJ01000017.1.
DR   RefSeq; YP_031533.1; NC_005945.1.
DR   AlphaFoldDB; Q81JL2; -.
DR   SMR; Q81JL2; -.
DR   STRING; 260799.BAS5296; -.
DR   TCDB; 9.B.33.1.1; the sensor histidine kinase (shk) family.
DR   DNASU; 1085432; -.
DR   EnsemblBacteria; AAP29324; AAP29324; BA_5692.
DR   EnsemblBacteria; AAT34850; AAT34850; GBAA_5692.
DR   GeneID; 45025267; -.
DR   KEGG; ban:BA_5692; -.
DR   KEGG; bar:GBAA_5692; -.
DR   KEGG; bat:BAS5296; -.
DR   PATRIC; fig|198094.11.peg.5654; -.
DR   eggNOG; COG3275; Bacteria.
DR   HOGENOM; CLU_020473_3_3_9; -.
DR   OMA; SHFFRSN; -.
DR   Proteomes; UP000000427; Chromosome.
DR   Proteomes; UP000000594; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0000155; F:phosphorelay sensor kinase activity; IEA:InterPro.
DR   GO; GO:0071555; P:cell wall organization; IEA:InterPro.
DR   Gene3D; 3.30.450.40; -; 1.
DR   Gene3D; 3.30.565.10; -; 1.
DR   InterPro; IPR003018; GAF.
DR   InterPro; IPR029016; GAF-like_dom_sf.
DR   InterPro; IPR003594; HATPase_C.
DR   InterPro; IPR036890; HATPase_C_sf.
DR   InterPro; IPR005467; His_kinase_dom.
DR   InterPro; IPR010559; Sig_transdc_His_kin_internal.
DR   InterPro; IPR011620; Sig_transdc_His_kinase_LytS_TM.
DR   Pfam; PF07694; 5TM-5TMR_LYT; 1.
DR   Pfam; PF02518; HATPase_c; 1.
DR   Pfam; PF06580; His_kinase; 1.
DR   SMART; SM00065; GAF; 1.
DR   SMART; SM00387; HATPase_c; 1.
DR   SUPFAM; SSF55874; SSF55874; 1.
DR   PROSITE; PS50109; HIS_KIN; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell membrane; Kinase; Membrane; Nucleotide-binding;
KW   Phosphoprotein; Reference proteome; Transferase; Transmembrane;
KW   Transmembrane helix; Two-component regulatory system.
FT   CHAIN           1..589
FT                   /note="Sensor protein LytS"
FT                   /id="PRO_0000074787"
FT   TRANSMEM        4..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        45..67
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        87..109
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        116..138
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        153..172
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        184..206
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          235..360
FT                   /note="GAF"
FT   DOMAIN          360..577
FT                   /note="Histidine kinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT   MOD_RES         387
FT                   /note="Phosphohistidine; by autocatalysis"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
SQ   SEQUENCE   589 AA;  65276 MW;  0D680698090D3379 CRC64;
     MLNLVLMMIE RVGLIVILGF LLSHIKTFRR LLHKQDGYVD KLKLICIFSV FTIVSNYTGI
     EIAGNTIMNE NWLQGVSSSS TIANTRIMGV GISGLLGGPI VGIGVGSIAG IHRYMLGGTT
     ALSCAISSIL AGVITGYIGY IFKKYNRTIT PKFSAVLSVF IVSLEMIMIL LIVEDGISIV
     KTIAIPMILV NSFGSFILLS MIQAILRQEE NAKALQTHKV LRIADKTLPY FRQGLTEESC
     KHVAQIIHRF TGTDAVSLTD TEKILAHVGL ASDHHIPSHS LITGLSKEVL HTGKIMKAKS
     REVINCQHEG CPLQAAIVIP LTSHGNTIGT LKLYFKNPNQ LSRVEEELAE GLAKIFSTQL
     ELGEAELQSK LLQDAEIKAL QAQINPHFLF NAINTVSALC RTDVEKARKL LLQLSVYFRC
     NLQGARQLLI PLEQELNHVQ AYLSLEQARF PNKYEVKMYI EDELKTTLVP PFVLQLLVEN
     ALRHAFPKKQ PVCEVEVHVF EKEGMVHFEV KDNGQGIEEE RLEQLGKMVV SSKKGTGTAL
     YNINERLIGL FGKETMLHIE SEVNEGTEIT FVIPKKVGEE EQIVKSISS