ID   LYTS_STAS1              Reviewed;         591 AA.
AC   Q4A009;
DT   26-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   25-MAY-2022, entry version 115.
DE   RecName: Full=Sensor protein LytS;
DE            EC=2.7.13.3;
DE   AltName: Full=Autolysin sensor kinase;
GN   Name=lytS; OrderedLocusNames=SSP0464;
OS   Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM
OS   20229 / NCIMB 8711 / NCTC 7292 / S-41).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=342451;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15305 / DSM 20229 / NCIMB 8711 / NCTC 7292 / S-41;
RX   PubMed=16135568; DOI=10.1073/pnas.0502950102;
RA   Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M.,
RA   Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T.;
RT   "Whole genome sequence of Staphylococcus saprophyticus reveals the
RT   pathogenesis of uncomplicated urinary tract infection.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005).
CC   -!- FUNCTION: Member of the two-component regulatory system LytR/LytS that
CC       probably regulates genes involved in cell wall metabolism.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC         histidine.; EC=2.7.13.3;
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC       protein {ECO:0000250}.
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DR   EMBL; AP008934; BAE17609.1; -; Genomic_DNA.
DR   RefSeq; WP_011302423.1; NZ_MTGA01000036.1.
DR   AlphaFoldDB; Q4A009; -.
DR   SMR; Q4A009; -.
DR   STRING; 342451.SSP0464; -.
DR   EnsemblBacteria; BAE17609; BAE17609; SSP0464.
DR   KEGG; ssp:SSP0464; -.
DR   PATRIC; fig|342451.11.peg.469; -.
DR   eggNOG; COG3275; Bacteria.
DR   HOGENOM; CLU_020473_3_3_9; -.
DR   OMA; SHFFRSN; -.
DR   OrthoDB; 1031920at2; -.
DR   Proteomes; UP000006371; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0000155; F:phosphorelay sensor kinase activity; IEA:InterPro.
DR   GO; GO:0071555; P:cell wall organization; IEA:InterPro.
DR   Gene3D; 3.30.450.40; -; 1.
DR   Gene3D; 3.30.565.10; -; 1.
DR   InterPro; IPR003018; GAF.
DR   InterPro; IPR029016; GAF-like_dom_sf.
DR   InterPro; IPR003594; HATPase_C.
DR   InterPro; IPR036890; HATPase_C_sf.
DR   InterPro; IPR010559; Sig_transdc_His_kin_internal.
DR   InterPro; IPR011620; Sig_transdc_His_kinase_LytS_TM.
DR   Pfam; PF07694; 5TM-5TMR_LYT; 1.
DR   Pfam; PF13492; GAF_3; 1.
DR   Pfam; PF02518; HATPase_c; 1.
DR   Pfam; PF06580; His_kinase; 1.
DR   SMART; SM00065; GAF; 1.
DR   SMART; SM00387; HATPase_c; 1.
DR   SUPFAM; SSF55874; SSF55874; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell membrane; Kinase; Membrane; Nucleotide-binding;
KW   Phosphoprotein; Reference proteome; Transferase; Transmembrane;
KW   Transmembrane helix; Two-component regulatory system.
FT   CHAIN           1..591
FT                   /note="Sensor protein LytS"
FT                   /id="PRO_0000292217"
FT   TRANSMEM        2..22
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        42..62
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        88..108
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        114..134
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        151..171
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        185..205
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          379..461
FT                   /note="Histidine kinase"
FT   MOD_RES         390
FT                   /note="Phosphohistidine; by autocatalysis"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   591 AA;  65698 MW;  16A8475212D0CE68 CRC64;
     MLNLFILLLE RVGLIILIAY ILMNINHFKT MMNEREKRRS QWQLIIIFGC FSMISNFTGI
     QIRGDEIING TVYNHLDPDA SLANTRVLTI GVSGLIGGPF VALAVAVISG MYRVYIGGAD
     AYIYLISSIF IALISGYFGY KAMRANRYPT IVKGACIGGT TEIIQMLCIL LFSDNTEHAW
     TLVKLIAIPM ISINSIGTAI FLSIILSTIK QEEETRAIQT HDVLQLANQT LPYFRSGLNE
     QSAKKAAEII LNLMRVSAVA ITNRKDILTH VGVASDHHVA QKAIITNLSK RAIQSGTLKE
     AYSSEEIGCN HPGCPLEAAI VVPLRVKNDV VGTLKLYFTN KYDVNYSDKQ LATGLAEIFS
     SQLELGQAET QSALIRDAEI KSLQAQVNPH FFFNAINTIS ALIRIDSEKA RELLLQLSQF
     FRSNLQGARN NTISLEKELQ QVESYLSLEQ ARYPDRFNVS FDIDRTCYGA LVPPFAIQIL
     VENAIKHAFK NRKYNNEIIV KAHKAQTGLV ISVSDNGHGI PYEKLDKIGK TSVHSESGTG
     SALENLNRRL DGLFGYEASL QIHSDHQGTQ VSCTIPYHNL EEEKIESNHC R