ID   LYZL4_BOVIN             Reviewed;         145 AA.
AC   Q2T9N7;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=Lysozyme-like protein 4;
DE            Short=Lysozyme-4;
DE   Flags: Precursor;
GN   Name=LYZL4;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Liver;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: May be involved in fertilization (By similarity). Has no
CC       detectable bacteriolytic and lysozyme activities in vitro (By
CC       similarity). {ECO:0000250|UniProtKB:D4ABW7,
CC       ECO:0000250|UniProtKB:Q9D925}.
CC   -!- SUBUNIT: Monomer. {ECO:0000250|UniProtKB:Q9D925}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q9D925}.
CC       Cytoplasmic vesicle, secretory vesicle, acrosome
CC       {ECO:0000250|UniProtKB:Q9D925}. Cell projection, cilium, flagellum
CC       {ECO:0000250|UniProtKB:Q9D925}. Note=Found in the principal piece of
CC       sperm tail. {ECO:0000250|UniProtKB:Q9D925}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 22 family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00680}.
CC   -!- CAUTION: Although it belongs to the glycosyl hydrolase 22 family, Gly-
CC       72 is present instead of the conserved Asp which is an active site
CC       residue. It is therefore expected that this protein lacks hydrolase
CC       activity. {ECO:0000305}.
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DR   EMBL; BC111339; AAI11340.1; -; mRNA.
DR   RefSeq; NP_001071427.1; NM_001077959.1.
DR   AlphaFoldDB; Q2T9N7; -.
DR   SMR; Q2T9N7; -.
DR   STRING; 9913.ENSBTAP00000024756; -.
DR   CAZy; GH22; Glycoside Hydrolase Family 22.
DR   PaxDb; Q2T9N7; -.
DR   Ensembl; ENSBTAT00000024756; ENSBTAP00000024756; ENSBTAG00000018601.
DR   GeneID; 523887; -.
DR   KEGG; bta:523887; -.
DR   CTD; 131375; -.
DR   VEuPathDB; HostDB:ENSBTAG00000018601; -.
DR   VGNC; VGNC:31117; LYZL4.
DR   eggNOG; ENOG502SSER; Eukaryota.
DR   GeneTree; ENSGT00940000162293; -.
DR   InParanoid; Q2T9N7; -.
DR   OMA; AWPSWSL; -.
DR   OrthoDB; 1551203at2759; -.
DR   Proteomes; UP000009136; Chromosome 22.
DR   Bgee; ENSBTAG00000018601; Expressed in semen and 15 other tissues.
DR   ExpressionAtlas; Q2T9N7; baseline.
DR   GO; GO:0001669; C:acrosomal vesicle; ISS:UniProtKB.
DR   GO; GO:0005615; C:extracellular space; ISS:UniProtKB.
DR   GO; GO:0036126; C:sperm flagellum; ISS:UniProtKB.
DR   GO; GO:0003796; F:lysozyme activity; IEA:InterPro.
DR   GO; GO:0009566; P:fertilization; ISS:UniProtKB.
DR   GO; GO:0007342; P:fusion of sperm to egg plasma membrane involved in single fertilization; IBA:GO_Central.
DR   InterPro; IPR001916; Glyco_hydro_22.
DR   InterPro; IPR019799; Glyco_hydro_22_CS.
DR   InterPro; IPR000974; Glyco_hydro_22_lys.
DR   InterPro; IPR023346; Lysozyme-like_dom_sf.
DR   InterPro; IPR030062; LYZL4.
DR   PANTHER; PTHR11407; PTHR11407; 1.
DR   PANTHER; PTHR11407:SF21; PTHR11407:SF21; 1.
DR   Pfam; PF00062; Lys; 1.
DR   PRINTS; PR00137; LYSOZYME.
DR   PRINTS; PR00135; LYZLACT.
DR   SMART; SM00263; LYZ1; 1.
DR   SUPFAM; SSF53955; SSF53955; 1.
DR   PROSITE; PS00128; GLYCOSYL_HYDROL_F22_1; 1.
DR   PROSITE; PS51348; GLYCOSYL_HYDROL_F22_2; 1.
PE   2: Evidence at transcript level;
KW   Cell projection; Cilium; Cytoplasmic vesicle; Disulfide bond;
KW   Fertilization; Flagellum; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..145
FT                   /note="Lysozyme-like protein 4"
FT                   /id="PRO_0000287124"
FT   DOMAIN          20..145
FT                   /note="C-type lysozyme"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00680"
FT   ACT_SITE        54
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00680"
FT   DISULFID        25..143
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00680"
FT   DISULFID        49..130
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00680"
FT   DISULFID        84..95
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00680"
FT   DISULFID        91..109
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00680"
SQ   SEQUENCE   145 AA;  16293 MW;  6E7AAFBF8448A44F CRC64;
     MKASVVLSLI GYLVVPSDTA VLGRCVVAKK LHEGGLSDFE GYSLENWVCL AYFESKFNPM
     AVYENSRSDF IGYGLFQIRN HDWCDHGRNR CHMSCSALLN PDLKKTIECA KTIVKGKRGM
     GAWPSWTLNC QHSDTLARWL DGCKL