ID   LYZL6_BOVIN             Reviewed;         148 AA.
AC   Q29RT1;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   04-APR-2006, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=Lysozyme-like protein 6;
DE            EC=3.2.1.17;
DE   Flags: Precursor;
GN   Name=LYZL6;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Testis;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: May be involved sperm-egg plasma membrane adhesion and fusion
CC       during fertilization. Exhibits bacteriolytic activity in vitro against
CC       Micrococcus luteus and Staphylococcus aureus. Shows weak bacteriolytic
CC       activity against Gram-positive bacteria at physiological pH.
CC       Bacteriolytic activity is pH-dependent, with a maximum at around pH 5.6
CC       (By similarity). {ECO:0000250|UniProtKB:O75951}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic
CC         acid and N-acetyl-D-glucosamine residues in a peptidoglycan and
CC         between N-acetyl-D-glucosamine residues in chitodextrins.;
CC         EC=3.2.1.17;
CC   -!- SUBUNIT: Monomer. {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:O75951}. Cell
CC       surface {ECO:0000250|UniProtKB:O75951}. Cell projection, cilium,
CC       flagellum {ECO:0000250|UniProtKB:Q9DA11}. Note=Detected in the post-
CC       acrosomal area and midpiece of mature spermatozoa.
CC       {ECO:0000250|UniProtKB:Q9DA11}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 22 family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00680}.
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DR   EMBL; BC114039; AAI14040.1; -; mRNA.
DR   RefSeq; NP_001039931.1; NM_001046466.1.
DR   AlphaFoldDB; Q29RT1; -.
DR   SMR; Q29RT1; -.
DR   STRING; 9913.ENSBTAP00000000032; -.
DR   CAZy; GH22; Glycoside Hydrolase Family 22.
DR   PaxDb; Q29RT1; -.
DR   GeneID; 540048; -.
DR   KEGG; bta:540048; -.
DR   CTD; 57151; -.
DR   eggNOG; ENOG502SCGK; Eukaryota.
DR   InParanoid; Q29RT1; -.
DR   OrthoDB; 1551203at2759; -.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0001669; C:acrosomal vesicle; IBA:GO_Central.
DR   GO; GO:0009986; C:cell surface; IEA:UniProtKB-SubCell.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0036126; C:sperm flagellum; IBA:GO_Central.
DR   GO; GO:0097225; C:sperm midpiece; ISS:UniProtKB.
DR   GO; GO:0097524; C:sperm plasma membrane; ISS:UniProtKB.
DR   GO; GO:0003796; F:lysozyme activity; IBA:GO_Central.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; ISS:UniProtKB.
DR   GO; GO:0009566; P:fertilization; ISS:UniProtKB.
DR   GO; GO:0007342; P:fusion of sperm to egg plasma membrane involved in single fertilization; ISS:UniProtKB.
DR   GO; GO:0008152; P:metabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR001916; Glyco_hydro_22.
DR   InterPro; IPR019799; Glyco_hydro_22_CS.
DR   InterPro; IPR000974; Glyco_hydro_22_lys.
DR   InterPro; IPR023346; Lysozyme-like_dom_sf.
DR   InterPro; IPR030063; LYZL6.
DR   PANTHER; PTHR11407; PTHR11407; 1.
DR   PANTHER; PTHR11407:SF9; PTHR11407:SF9; 1.
DR   Pfam; PF00062; Lys; 1.
DR   PRINTS; PR00137; LYSOZYME.
DR   PRINTS; PR00135; LYZLACT.
DR   SMART; SM00263; LYZ1; 1.
DR   SUPFAM; SSF53955; SSF53955; 1.
DR   PROSITE; PS00128; GLYCOSYL_HYDROL_F22_1; 1.
DR   PROSITE; PS51348; GLYCOSYL_HYDROL_F22_2; 1.
PE   2: Evidence at transcript level;
KW   Antimicrobial; Bacteriolytic enzyme; Cell projection; Cilium;
KW   Disulfide bond; Fertilization; Flagellum; Glycosidase; Hydrolase;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..148
FT                   /note="Lysozyme-like protein 6"
FT                   /id="PRO_0000287125"
FT   DOMAIN          20..148
FT                   /note="C-type lysozyme"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00680"
FT   ACT_SITE        54
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00680"
FT   ACT_SITE        71
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00680"
FT   DISULFID        25..145
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00680"
FT   DISULFID        49..133
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00680"
FT   DISULFID        83..98
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00680"
FT   DISULFID        94..112
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00680"
SQ   SEQUENCE   148 AA;  16559 MW;  DCE7222314F37402 CRC64;
     MTSPLLISLA SCLVAVNQAS LIGRCDLAKV LHQEDLDGFE GYSLTDWLCL AFVESDFNIT
     KVNENTDGSF DYGIFQINSH YWCNDYQSRT ENNCQVDCQE LLSPNLLAII NCAKKIVSGA
     GGMKNWVKWR LHCAGRPLSY WMTGCHLA