ID   L_BDV1                  Reviewed;        1711 AA.
AC   Q8JMN0; Q88627;
DT   08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=RNA-directed RNA polymerase L;
DE            Short=Protein L;
DE            EC=2.7.7.48;
DE   AltName: Full=Large structural protein;
DE   AltName: Full=Replicase;
DE   AltName: Full=Transcriptase;
GN   Name=L;
OS   Borna disease virus 1 (BoDV-1).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC   Monjiviricetes; Mononegavirales; Bornaviridae; Orthobornavirus.
OX   NCBI_TaxID=1714621;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
OH   NCBI_TaxID=9352; Bradypodidae (three-fingered sloths).
OH   NCBI_TaxID=9925; Capra hircus (Goat).
OH   NCBI_TaxID=9850; Cervidae (deer).
OH   NCBI_TaxID=109474; Crocidura leucodon (Bicoloured white-toothed shrew) (Celebes shrew).
OH   NCBI_TaxID=9788; Equidae (horses).
OH   NCBI_TaxID=9685; Felis catus (Cat) (Felis silvestris catus).
OH   NCBI_TaxID=56798; Hexaprotodon liberiensis (Pygmy hippopotamus) (Choeropsis liberiensis).
OH   NCBI_TaxID=9844; Lama glama (Llama).
OH   NCBI_TaxID=9986; Oryctolagus cuniculus (Rabbit).
OH   NCBI_TaxID=9940; Ovis aries (Sheep).
OH   NCBI_TaxID=8801; Struthio camelus (Common ostrich).
OH   NCBI_TaxID=9455; Varecia variegata (Black-and-white ruffed lemur) (Lemur variegatus).
OH   NCBI_TaxID=30538; Vicugna pacos (Alpaca) (Lama pacos).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=CRP3A;
RX   PubMed=12163584; DOI=10.1128/jvi.76.17.8650-8658.2002;
RA   Nishino Y., Kobasa D., Rubin S.A., Pletnikov M.V., Carbone K.M.;
RT   "Enhanced neurovirulence of borna disease virus variants associated with
RT   nucleotide changes in the glycoprotein and L polymerase genes.";
RL   J. Virol. 76:8650-8658(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=7906311; DOI=10.1128/jvi.68.3.1382-1396.1994;
RA   Cubitt B., Oldstone C., de la Torre J.C.;
RT   "Sequence and genome organization of Borna disease virus.";
RL   J. Virol. 68:1382-1396(1994).
RN   [3]
RP   REVIEW.
RX   PubMed=11815287; DOI=10.2741/a789;
RA   Ikuta K., Ibrahim M.S., Kobayashi T., Tomonaga K.;
RT   "Borna disease virus and infection in humans.";
RL   Front. Biosci. 7:470-495(2002).
CC   -!- FUNCTION: Displays RNA-directed RNA polymerase activity. The viral mRNA
CC       guanylyl transferase displays a different biochemical reaction than the
CC       cellular enzyme. The template is composed of the viral RNA tightly
CC       encapsidated by the nucleoprotein (N). Functions either as
CC       transcriptase or as replicase. The transcriptase synthesizes
CC       subsequently three subgenomic RNAs, assuring their capping and
CC       polyadenylation by a stuttering mechanism. In replicase mode, the
CC       polymerase replicates the whole viral genome without recognizing the
CC       transcriptional signals. {ECO:0000250|UniProtKB:P52639}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- SUBUNIT: Interacts with the P protein. {ECO:0000250|UniProtKB:P52639}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Host nucleus.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=3;
CC       Name=Large structural protein;
CC         IsoId=Q8JMN0-1; Sequence=Displayed;
CC       Name=Matrix protein;
CC         IsoId=P0C794-1; Sequence=External;
CC       Name=Envelope glycoprotein p57 precursor;
CC         IsoId=Q8BB27-1; Sequence=External;
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA20667.2; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AY114161; AAM68139.1; -; Genomic_RNA.
DR   EMBL; L27077; AAA20667.2; ALT_INIT; Genomic_RNA.
DR   SMR; Q8JMN0; -.
DR   PRIDE; Q8JMN0; -.
DR   Proteomes; UP000117149; Genome.
DR   Proteomes; UP000185272; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004482; F:mRNA (guanine-N7-)-methyltransferase activity; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   InterPro; IPR026890; Mononeg_mRNAcap.
DR   InterPro; IPR014023; Mononeg_RNA_pol_cat.
DR   Pfam; PF14318; Mononeg_mRNAcap; 1.
DR   Pfam; PF00946; Mononeg_RNA_pol; 1.
DR   PROSITE; PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PE   3: Inferred from homology;
KW   Alternative splicing; ATP-binding; Host nucleus; Methyltransferase;
KW   mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotide-binding;
KW   Nucleotidyltransferase; RNA-directed RNA polymerase;
KW   S-adenosyl-L-methionine; Transferase; Viral RNA replication; Virion.
FT   CHAIN           1..1711
FT                   /note="RNA-directed RNA polymerase L"
FT                   /id="PRO_0000405349"
FT   DOMAIN          543..715
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT   MOTIF           844..852
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000250|UniProtKB:P52639"
FT   CONFLICT        1..5
FT                   /note="MSFHA -> LCFLT (in Ref. 2; AAA20667)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        902
FT                   /note="P -> A (in Ref. 2; AAA20667)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1069
FT                   /note="K -> T (in Ref. 2; AAA20667)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1222
FT                   /note="F -> V (in Ref. 2; AAA20667)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1364
FT                   /note="S -> Y (in Ref. 2; AAA20667)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1711 AA;  191646 MW;  10338AFE9A9CAD24 CRC64;
     MSFHASLLRE EETPRPVAGI NRTDQSLKNP LLGTEVSFCL KSSSLPHHVR ALGQIKAKNL
     ASCDYYLLFR QVVLPPEVYP IGVLIRAAEA ILTVIVSAWK LDHMTKTLYS SVRYALTNPR
     VRAQLELHIA YQRIVGQVSY SREADIGPKR LGNMSLQFIQ SLVIATIDTT SCLMTYNHFL
     AAADTAKSRC HLLIASVVQG ALWEQGSFLD HIINMIDTID SINLPHDDYF TIIKSISPYS
     QGLVMGRHNV SVSSDFASVF TIPESCPQLD SLLKKLLQLD PVLLLMVSSV QKSWYFPEIR
     MVDGSREQLH KMRVELETPQ ALLSYGHTLL SIFRAEFIKG YVSKNAKWPP VHLLPGCDKS
     IKNARELGRW SPVFDRRWQL FAKVVILRIA DLDMDPDFND IVSDKAIISS RRDWVFEYNA
     AAFWKKYSER LERPPARSGP SRLVNALIDG RLDNIPALLE PFYRGAVEFE DRLTVLVPKE
     KELKVKGRFF SKQTLAIRIY QVVAEAALKN EVMPYLKTHS MTMSSTALTH LLNRLSHTIT
     KGDSFVINLD YSSWCNGFRP ELQAPLCRQL DQMFNCGYFF RTGCTLPCFT TFIIQDRFNP
     PYSFRGEPVE DGVTCAVGTK TMGEGMRQKL WTILTSCWEI IALREINVTF NILGQGDNQT
     IIVHKSASQN NQLLAERALG ALYKHARLAG HNLKVEECWV SDCLYEYGKK LFFRGVPVPG
     CLKQLSRVTD STGELFPNLY SKLACLTSSC LSAAMADTSP WVALATGVCL YLIELYVELP
     PAIMQDESLL TTLCLVGPSI GGLPTPATLP SVFFRGMSDP LPFQLALLQT LIKTTGVTCS
     LVNRVVKLRI APYPDWLSLV TDPTSLNIAQ VYRPERQIRR WIEEAIATSS HSSRIATFFQ
     QPLTEMAQLL ARDLSTMMPL RPRDMSALFA LSNVAYGLSI IDLFQKSSTV VSASQAVHIE
     DVALESVRYK ESIIQGLLDT TEGYNMQPYL EGCTYLAAKQ LRRLTWGRDL VGVTMPFVAE
     QFHPHSSVGA KAELYLDAII YCPQETLRSH HLTTRGDQPL YLGSNTAVKV QRGEITGLTK
     SRAANLVKDT LVLHQWYKVR KVTDPHLNTL MARFLLEKGY TSDARPSIQG GTLTHRLPSR
     GDSRQGLTGY VNILSTWLRF SSDYLHSFSK SSDDYTIHFQ HVFTYGCLYA DSVIRSGGVI
     STPYLLSASC KTCFEKIDSE EFVLACEPQY RGAEWLISKP VTVPEQIIDA EVEFDPCVSA
     SYCLGILIGK SFLVDIRASG HDIMEQRTWA NLERFSVSDM QKLPWSIVIR SLWRFLIGAR
     LLQFEKAGLI RMLYAATGPT FSFLMKVFQD SALLMDCAPL DRLSPRINFH SRGDLVAKLV
     LLPFINPGIV EIEVSRINSK YHAVSEANMD LYIAAAKSVG VKPTQFVEET NDFTARGHHH
     GCYSLSWSKS RNQSQVLKMV VRKLKLCVLY IYPTVDPAVA LDLCHLPALT IILVLGGDPA
     YYERLLEMDL CGAVSSRVDI PHSLAARTHR GFTIGPDAGP GVIRLDKLES VCYAHPCLEE
     LEFNAYLDSE LVDISDMCCL PLATPCKALF RPVYRSLQSF RLALMDNYSF VMDLITIRGV
     DIRPHLEEFD ELLVVGQHIL GQPVLVEVVY YVGVVGKRPV LARHPWSADL KRITVGGRAP
     CPSAAGLRDE DCRGSLLVGL PAGLTQLLVV D