L_BDVV
ID L_BDVV Reviewed; 1711 AA.
AC P52639; Q912Z6;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 2.
DT 23-FEB-2022, entry version 110.
DE RecName: Full=RNA-directed RNA polymerase L;
DE Short=Protein L;
DE EC=2.7.7.48;
DE AltName: Full=Large structural protein;
DE AltName: Full=Replicase;
DE AltName: Full=Transcriptase;
GN Name=L;
OS Borna disease virus (strain V) (BDV).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC Monjiviricetes; Mononegavirales; Bornaviridae; unclassified Bornaviridae.
OX NCBI_TaxID=928296;
OH NCBI_TaxID=9913; Bos taurus (Bovine).
OH NCBI_TaxID=9352; Bradypodidae (three-fingered sloths).
OH NCBI_TaxID=9925; Capra hircus (Goat).
OH NCBI_TaxID=9850; Cervidae (deer).
OH NCBI_TaxID=109474; Crocidura leucodon (Bicoloured white-toothed shrew) (Celebes shrew).
OH NCBI_TaxID=9788; Equidae (horses).
OH NCBI_TaxID=9685; Felis catus (Cat) (Felis silvestris catus).
OH NCBI_TaxID=56798; Hexaprotodon liberiensis (Pygmy hippopotamus) (Choeropsis liberiensis).
OH NCBI_TaxID=9844; Lama glama (Llama).
OH NCBI_TaxID=9986; Oryctolagus cuniculus (Rabbit).
OH NCBI_TaxID=9940; Ovis aries (Sheep).
OH NCBI_TaxID=8801; Struthio camelus (Common ostrich).
OH NCBI_TaxID=9455; Varecia variegata (Black-and-white ruffed lemur) (Lemur variegatus).
OH NCBI_TaxID=30538; Vicugna pacos (Alpaca) (Lama pacos).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=8183914; DOI=10.1073/pnas.91.10.4362;
RA Briese T., Schneemann A., Lewis A.J., Park Y.-S., Kim S., Ludwig H.,
RA Lipkin W.I.;
RT "Genomic organization of Borna disease virus.";
RL Proc. Natl. Acad. Sci. U.S.A. 91:4362-4366(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=V/FR;
RX PubMed=11602780; DOI=10.1099/0022-1317-82-11-2681;
RA Pleschka S., Staeheli P., Kolodziejek J., Richt J.A., Nowotny N.,
RA Schwemmle M.;
RT "Conservation of coding potential and terminal sequences in four different
RT isolates of Borna disease virus.";
RL J. Gen. Virol. 82:2681-2690(2001).
RN [3]
RP INTERACTION WITH P PROTEIN.
RX PubMed=10756058; DOI=10.1128/jvi.74.9.4425-4428.2000;
RA Walker M.P., Jordan I., Briese T., Fischer N., Lipkin W.I.;
RT "Expression and characterization of the Borna disease virus polymerase.";
RL J. Virol. 74:4425-4428(2000).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=12134049; DOI=10.1128/jvi.76.16.8460-8467.2002;
RA Walker M.P., Lipkin W.I.;
RT "Characterization of the nuclear localization signal of the borna disease
RT virus polymerase.";
RL J. Virol. 76:8460-8467(2002).
RN [5]
RP REVIEW.
RX PubMed=11815287; DOI=10.2741/a789;
RA Ikuta K., Ibrahim M.S., Kobayashi T., Tomonaga K.;
RT "Borna disease virus and infection in humans.";
RL Front. Biosci. 7:470-495(2002).
RN [6]
RP FUNCTION.
RX PubMed=14557662; DOI=10.1128/jvi.77.21.11781-11789.2003;
RA Schneider U., Naegele M., Staeheli P., Schwemmle M.;
RT "Active borna disease virus polymerase complex requires a distinct
RT nucleoprotein-to-phosphoprotein ratio but no viral X protein.";
RL J. Virol. 77:11781-11789(2003).
RN [7]
RP FUNCTION.
RX PubMed=18653450; DOI=10.1128/jvi.00639-08;
RA Poenisch M., Wille S., Staeheli P., Schneider U.;
RT "Polymerase read-through at the first transcription termination site
RT contributes to regulation of borna disease virus gene expression.";
RL J. Virol. 82:9537-9545(2008).
RN [8]
RP REVIEW.
RX PubMed=22138959; DOI=10.1038/nrmicro2675;
RA Decroly E., Ferron F., Lescar J., Canard B.;
RT "Conventional and unconventional mechanisms for capping viral mRNA.";
RL Nat. Rev. Microbiol. 10:51-65(2011).
CC -!- FUNCTION: Displays RNA-directed RNA polymerase activity. The mRNA
CC guanylyl transferase and mRNA (guanine-N(7)-)-methyltransferase
CC activities are supposedly provided by cellular enzymes
CC (PubMed:22138959). The template is composed of the viral RNA tightly
CC encapsidated by the nucleoprotein (N). Functions either as
CC transcriptase or as replicase. The transcriptase synthesizes
CC subsequently three subgenomic RNAs, assuring their capping and
CC polyadenylation by a stuttering mechanism. In replicase mode, the
CC polymerase replicates the whole viral genome without recognizing the
CC transcriptional signals. {ECO:0000303|PubMed:22138959,
CC ECO:0000305|PubMed:14557662, ECO:0000305|PubMed:18653450}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC -!- SUBUNIT: Interacts with the P protein. {ECO:0000269|PubMed:10756058}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Host nucleus
CC {ECO:0000269|PubMed:12134049}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=Large structural protein;
CC IsoId=P52639-1; Sequence=Displayed;
CC Name=Matrix protein;
CC IsoId=P0C795-1; Sequence=External;
CC Name=Envelope glycoprotein p57 precursor;
CC IsoId=P52638-1; Sequence=External;
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA20228.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; U04608; AAA20228.1; ALT_INIT; Genomic_RNA.
DR EMBL; AJ311521; CAC70639.1; -; Genomic_RNA.
DR RefSeq; NP_042024.3; NC_001607.1.
DR SMR; P52639; -.
DR GeneID; 26799167; -.
DR KEGG; vg:26799167; -.
DR Proteomes; UP000007804; Genome.
DR Proteomes; UP000124375; Genome.
DR GO; GO:0042025; C:host cell nucleus; IDA:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004482; F:mRNA (guanine-N7-)-methyltransferase activity; IEA:InterPro.
DR GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR InterPro; IPR026890; Mononeg_mRNAcap.
DR InterPro; IPR014023; Mononeg_RNA_pol_cat.
DR Pfam; PF14318; Mononeg_mRNAcap; 1.
DR Pfam; PF00946; Mononeg_RNA_pol; 1.
DR PROSITE; PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; ATP-binding; Host nucleus; mRNA capping;
KW mRNA processing; Multifunctional enzyme; Nucleotide-binding;
KW Nucleotidyltransferase; Reference proteome; RNA-directed RNA polymerase;
KW S-adenosyl-L-methionine; Transferase; Viral RNA replication; Virion.
FT CHAIN 1..1711
FT /note="RNA-directed RNA polymerase L"
FT /id="PRO_0000079207"
FT DOMAIN 543..715
FT /note="RdRp catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT MOTIF 844..852
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000269|PubMed:12134049"
FT VARIANT 1526
FT /note="A -> G"
SQ SEQUENCE 1711 AA; 191734 MW; 554DF018E0E17877 CRC64;
MSFHASLLRE EETPRPVAGI NRTDQSLKNP LLGTEVSFCL KSSSLPHHVR ALGQIKARNL
ASCDYYLLFR QVVLPPEVYP IGVLIRAAEA ILTVIVSAWK LDHMTKTLYS SVRYALTNPR
VRAQLELHIA YQRIVGQVSY SREADIGPKR LGNMSLQFIQ SLVIATIDTT SCLMTYNHFL
AAADTAKSRC HLLIASVVQG ALWEQGSFLD HIINMIDIID SINLPHDDYF TIIKSIFPYS
QGLVMGRHNV SVSSDFASVF AIPELCPQLD SLLKKLLQLD PVLLLMVSSV QKSWYFPEIR
MVDGSREQLH KMRVELETPQ ALLSYGHTLL SIFRAEFIKG YVSKNAKWPP VHLLPGCDKS
IKNARELGRW SPAFDRRWQL FEKVVILRIA DLDMDPDFND IVSDKAIISS RRDWVFEYNA
AAFWKKYGER LERPPARSGP SRLVNALIDG RLDNIPALLE PFYRGAVEFE DRLTVLVPKE
KELKVKGRFF SKQTLAIRIY QVVAEAALKN EVMPYLKTHS MTMSSTALTH LLNRLSHTIT
KGDSFVINLD YSSWCNGFRP ELQAPICRQL DQMFNCGYFF RTGCTLPCFT TFIIQDRFNP
PYSLSGEPVE DGVTCAVGTK TMGEGMRQKL WTILTSCWEI IALREINVTF NILGQGDNQT
IIIHKSASQN NQLLAERALG ALYKHARLAG HNLKVEECWV SDCLYEYGKK LFFRGVPVPG
CLKQLSRVTD STGELFPNLY SKLACLTSSC LSAAMADTSP WVALATGVCL YLIELYVELP
PAIIQDESLL TTLCLVGPSI GGLPTPATLP SVFFRGMSDP LPFQLALLQT LIKTTGVTCS
LVNRVVKLRI APYPDWLSLV TDPTSLNIAQ VYRPERQIRR WIEEAIATSS HSSRIATFFQ
QPLTEMAQLL ARDLSTMMPL RPRDMSALFA LSNVAYGLSI IDLFQKSSTV VSASQAVHIE
DVALESVRYK ESIIQGLLDT TEGYNMQPYL EGCTYLAAKQ LRRLTWGRDL VGVTMPFVAE
QFHPHSSVGA KAELYLDAII YCPQETLRSH HLTTRGDQPL YLGSNTAVKV QRGEITGLAK
SRAANLVKDT LVLHQWYKVR KVTDPHLNTL MARFLLEKGY TSDARPSIQG GTLTHRLPSR
GDSRQGLTGY VNILSTWLRF SSDYLHSFSK SSDDYTIHFQ HVFTYGCLYA DSVIRSGGVI
STPYLLSASC KTCFEKIDSE EFVLACEPQY RGAEWLISKP VTVPEQITDA EVEFDPCVSA
SYCLGILIGK SFLVDIRASG HDIMEQRTWA NLERFSVSDM QKLPWSIVIR SLWRFLIGAR
LLQFEKAGLI RMLYAATGPT FSFLMKVFQD SALLMDCAPL DRLSPRINFH SRGDLVAKLV
LLPFINPGIV EIEVSGINSK YHAVSEANMD LYIAAAKSVG VKPTQFVEET NDFTARGHHH
GCYSLSWSKS RNQSQVLKMV VRKLKLCVLY IYPTVDPAVA LDLCHLPALT IILVLGGDPA
YYERLLEMDL CGAVSSRVDI PHSLAARTHR GFAVGPDAGP GVIRLDRLES VCYAHPCLEE
LEFNAYLDSE LVDISDMCCL PLATPCKALF RPIYRSLQSF RLALMDNYSF VMDLIMIRGL
DIRPHLEEFD ELLVVGQHIL GQPVLVEVVY YVGVVRKRPV LARHPWSADL KRITVGGRAP
CPSAARLRDE DCQGSLLVGL PAGLTQLLII D
