L_CPSVP
ID L_CPSVP Reviewed; 2416 AA.
AC Q6DN67;
DT 09-FEB-2010, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 1.
DT 29-SEP-2021, entry version 59.
DE RecName: Full=RNA-directed RNA polymerase L;
DE Short=Protein L;
DE AltName: Full=Large structural protein;
DE AltName: Full=Replicase;
DE AltName: Full=Transcriptase;
DE Includes:
DE RecName: Full=RNA-directed RNA polymerase;
DE EC=2.7.7.48;
DE Includes:
DE RecName: Full=mRNA (guanine-N(7))-methyltransferase;
DE EC=2.1.1.56;
DE Includes:
DE RecName: Full=mRNA guanylyltransferase;
DE EC=2.7.7.-;
GN Name=L;
OS Citrus psorosis virus (isolate Spain/P-121) (CPsV) (Citrus ringspot virus).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC Milneviricetes; Serpentovirales; Aspiviridae; Ophiovirus.
OX NCBI_TaxID=652963;
OH NCBI_TaxID=159033; Citrus aurantiifolia (Key lime) (Limonia aurantifolia).
OH NCBI_TaxID=2708; Citrus limon (Lemon) (Citrus medica var. limon).
OH NCBI_TaxID=37656; Citrus paradisi (Grapefruit).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=15449144; DOI=10.1007/s00705-004-0403-y;
RA Martin S., Lopez C., Garcia M.L., Naum-Ongania G., Grau O., Flores R.,
RA Moreno P., Guerri J.;
RT "The complete nucleotide sequence of a Spanish isolate of Citrus psorosis
RT virus: comparative analysis with other ophioviruses.";
RL Arch. Virol. 150:167-176(2005).
CC -!- FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl
CC transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A)
CC synthetase activities. The viral mRNA guanylyl transferase displays a
CC different biochemical reaction than the cellular enzyme. The template
CC is composed of the viral RNA tightly encapsidated by the nucleoprotein
CC (N). Functions either as transcriptase or as replicase (By similarity).
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 5'-end (5'-triphosphoguanosine)-(ribonucleoside) in mRNA +
CC S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-
CC triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:67008, Rhea:RHEA-COMP:17166, Rhea:RHEA-
CC COMP:17167, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:156461,
CC ChEBI:CHEBI:167617; EC=2.1.1.56;
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DR EMBL; AY654892; AAT72907.1; -; Genomic_DNA.
DR RefSeq; YP_089661.1; NC_006314.1.
DR PRIDE; Q6DN67; -.
DR GeneID; 3077255; -.
DR KEGG; vg:3077255; -.
DR Proteomes; UP000009269; Genome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004482; F:mRNA (guanine-N7-)-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR InterPro; IPR014023; Mononeg_RNA_pol_cat.
DR PROSITE; PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PE 3: Inferred from homology;
KW ATP-binding; Coiled coil; Methyltransferase; mRNA capping; mRNA processing;
KW Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase;
KW Reference proteome; RNA-directed RNA polymerase; S-adenosyl-L-methionine;
KW Transferase; Viral RNA replication.
FT CHAIN 1..2416
FT /note="RNA-directed RNA polymerase L"
FT /id="PRO_0000391479"
FT DOMAIN 698..864
FT /note="RdRp catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT COILED 27..59
FT /evidence="ECO:0000255"
FT COILED 1082..1115
FT /evidence="ECO:0000255"
SQ SEQUENCE 2416 AA; 280374 MW; F740508DB16D85D7 CRC64;
MEWSDEKASS KITLEDEIEI ITSDIVASED TIIELAKKEE EAKKALEARK GYAEEYIKLI
EEMKNYPSAY SILSGKKEEY IMLRTAQDHE KSLEPKKEKI KYTSMIKYWI KWDHLAYIGT
YVDRPALISR LDSPYKTITN ETIEDFLYRE DYPEKFGVRA KIDLFRLREF VGQEKWSSKI
KQGNQKETEI WVSYLKETKN KSKKLETLLE YGKNMADEEL LSNREKITGK MQKTYKEAWK
IEPSEDTQTS LWFINLLEII IFKIKMTYSQ LLNRIESVSE SMEREGRKII TKVNLDGSST
TTLVVNNAQT NEKIKTEIFI SNTFHAIKYN GVILIGDNTL LKFVLSTEEN ELNMNIVKDY
NFMKGEKSDN FFIDFMMRLK KVPQPLRTLI AATYETVCLM MADHKCSSST LPIIDAVIEL
MKLDKKIGEE LLSICMTSDG VXCIKASTLA KMNTYAEVNE LEGLRKYIQR TNRNHDVSKE
SIERLRCMMR MKIITNYIKK FGVVPQMTCN SGLLEQELNL MASGGSYNNH IIRNTSHYKD
VKLGKMLQPG NEVNISSRVI DKACTKDEYD FSGNSVKELV YYITKNDLVD LIDEIEIGKV
YEENKNQDRE TKCFFRKDKE LARMQKYKIC RLIEKEKELK TEGRFYGVAS FKLKIYISII
MEMIKRAMKL IPGQMMTMTE DERRTVMHRM SVMLEEKDAY TLFLDYSGHN TSQRPENNLF
ILEEIADMYG FEENSIERKR LTQVVYLFNE LEILYEHLFS DMVVWSRRQK GAIEGWFGPL
WGIQSQLMLD DMMVSMGIEK YIGTTYSDDS CGVFIKKNLD QEELNRMIEY IQSYSLKMGL
LVKLSQTQIT NGRCSMLKNH YYFDKPIDTS YKRIMSISPN SDILWGNDSE RVSVIDSAYT
SSVLRADDNY IQTVIRNFRI MIEIEKDVIR WALFYDLELD PRFINLRGSL STLGKIYRKI
VDEASELREL KEIENSMPNE DEIVTQFFLF HIKNRKLLKL TLALMYLPST CYGHALTSMV
DSFISGYSYS NVKRLSYVES LFNDKKDKVF IYSLVRLSEN AISYVGEPFP MTGGRYDTKT
ILKDELKQLL KHKVQNNELK EMLNSFSEDK EHLFKAVLVH TFEKCFSHRI ASKFYECSIF
NYINELYAKI NNSTTMSYLV GKKKMNKLWN KAWKVNHKIE YKFQGNIINE IKSDPSYFGL
IMGRNKVDKR IVQNNEMKYF RMNFLNIEEL PIIGKMSADN LTGQLRCIIK PMRVYSKIKG
LRLPGPVRTA INITKFDRDL EIEGMFQNKL IFMAYELVRY VKWMIFDMEK YNKQDNTQAI
KSLRKLTDIT IGTFSNAKIN DIEEAVVCPK GGRYFHRALS GGFNPKTGDL SSNMLSSRVE
VTGLDRLTNE TGGVDNNINL QLVITALKVQ LALIGYTNNE TISIGLDSDV KYFARDVTFM
LDQIKSEKEL ECDLGLSHRF DSMTLDKLNE KRTLYHNFSH FVSVNEDIAG KFIDHKTILP
SSKIEEISSF QSLNKYMQDM EILVPTQIPE NIMKELVPNL NSYGSIDRYL EEFYKFYKGL
NIIGNESFTK AVVRSIINKE LFDRKNIEGK DWLKEIELTG FSYGYRKSLL KIFIVSCCMV
FRVEEPTGNT LKITIFEDKT KINCLNNIKR IKNSEAHLHI NDKRISDLIN MAFPLVGYQL
KDVFVAVREI MEEHNGKIID GTKVIQYFNV ENTSYASQLE EENCGRISYS NIVLKPSDLS
DEKQFIASIK AFEMIATLNC KPQHVSSPTK SDVYPSAKAL LDYLINSNVV SVDDKIADIF
AGRGDFHLIM DNMGITHTSI SRNDGYNLIN RIPGMKEIKA NIDMTYSENY GQYLDHDVFI
LDISHFTGDP NNIIRMIDDI LAVGKKVIIR WNSIAPMCTK LLYNFVIKRH HKIDILMPTI
ESPGYIYLLF NGGFYEPKEI LEGENSKKEK KTYNENIITS NMVLELNRIR KTSILNCSGI
KVSDHTQELI SDDKLISILE ENESKSYISN DIEMRIEEGE KLEELLMVLN PSDKLLGLEN
IIRQEIRVEE AERGAKSLKY FKRLIAETKP ISGMKKIKLD KNFLKWMIRE NKMKLENDKL
IIVEGVKNGH CKEIKKTIDL IINDKNTSKM NQEAWTCVKA GINQFISVEG EETECDMKVI
LMMIQGSTEY KSRFFSYDRI LRLAHMASDA IKTGTMAESI LILSGLRSGV LSSLKKNKGK
ALRYDCLNIK LIMNRMLVLS EERNIIIQSS NLRLSEIVKD ILLFRKIGQG DKRVKKSIRG
KFKRGEKNNK HIISWFDEDE ELELKCTELL EQREIEEEIL KLREFDRLED YLESQGINEL
FSAITDELSK EGVRMEMTEE HLAQGIDEQA NILSEGFKSF LGVQDEEVEK NRNMTVEEMR
KLVDDDDDCE YLSDEE
