ID   L_EMARV                 Reviewed;        2293 AA.
AC   Q6Q305;
DT   13-JUL-2010, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 2.
DT   23-FEB-2022, entry version 66.
DE   RecName: Full=RNA-directed RNA polymerase L;
DE            Short=Protein L;
DE            EC=2.7.7.48;
DE   AltName: Full=Large structural protein;
DE   AltName: Full=Replicase;
DE   AltName: Full=Transcriptase;
GN   Name=RdRp;
OS   European mountain ash ringspot-associated virus (isolate Sorbus aucuparia)
OS   (EMARAV).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC   Ellioviricetes; Bunyavirales; Fimoviridae; Emaravirus.
OX   NCBI_TaxID=1980426;
OH   NCBI_TaxID=483436; Eriophyes pyri (pearleaf blister mite).
OH   NCBI_TaxID=36599; Sorbus aucuparia (European mountain ash) (Rowan).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=15449143; DOI=10.1007/s00705-004-0397-5;
RA   Benthack W., Mielke N., Buttner C., Muhlbach H.P.;
RT   "Double-stranded RNA pattern and partial sequence data indicate plant virus
RT   infection associated with the ringspot disease of European mountain ash
RT   (Sorbus aucuparia L.).";
RL   Arch. Virol. 150:37-52(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=17374780; DOI=10.1099/vir.0.82715-0;
RA   Mielke N., Muehlbach H.P.;
RT   "A novel, multipartite, negative-strand RNA virus is associated with the
RT   ringspot disease of European mountain ash (Sorbus aucuparia L.).";
RL   J. Gen. Virol. 88:1337-1346(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RA   Mielke N., Muehlbach H.-P.;
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: RNA-dependent RNA polymerase which is responsible for
CC       replication and transcription of the viral RNA genome. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AY563040; AAS73287.2; -; mRNA.
DR   RefSeq; YP_003104764.1; NC_013105.1.
DR   GeneID; 8317725; -.
DR   KEGG; vg:8317725; -.
DR   Proteomes; UP000006676; Genome.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   InterPro; IPR007099; RNA-dir_pol_NSvirus.
DR   InterPro; IPR007322; RNA_pol_bunyavir.
DR   Pfam; PF04196; Bunya_RdRp; 1.
DR   PROSITE; PS50525; RDRP_SSRNA_NEG_SEG; 1.
PE   2: Evidence at transcript level;
KW   Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW   RNA-directed RNA polymerase; Transferase; Viral RNA replication.
FT   CHAIN           1..2293
FT                   /note="RNA-directed RNA polymerase L"
FT                   /id="PRO_0000395604"
FT   DOMAIN          1111..1297
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
SQ   SEQUENCE   2293 AA;  265654 MW;  AC3F60877F5E2769 CRC64;
     MENIRKLEAQ RRKEYLRAET EIRNNNVFEK DILQKFLHIV GKKQRHYTIS SKKKEVQDIF
     NKCSTNPGFT KDVIDLAERI LYAPPNLNQI DFAMTIVSLI EMQRHDDLIH KLNELLVRSG
     MKVISFEFKL CDHFPFTNSI LTPDILFEDP YGSRYILEVK VRNKHTDLEH YYMRYKKVVG
     VHAKVGVFNL SQSGYMQHGD YKLSEKINLE SDDFDDILLC VELAGKIREK YIQYPQYFLY
     TLQSEVTNPD SFLDGFKTRL SDLSMFEEIK SGFGDYWNDI TYHMDNYSLI DNHDEVTEDL
     LNSTDDLTQY CNDLYDEFLD HSQSYSRQGK YGKTILRNSG LDNIIDKKNR SKYTITSKLK
     PSVYIPITKT IKLDTYGGSR LKFYKDAFIN IKCTGDSYSR SAYNLVDNVF NTSSIDLLMT
     KDDKIDPSLY YEVLDPGFIA HLHDDQVKYK KIAKVTNITS DMTILANNSF SIHCHTDQRL
     KDNICGYDKK HYDSTQKAKE CLDFSNSSLL LPSLGVHLSA IFKSEHHAGV YWNDLVTLGT
     DNLNHMTSDI PEEAQTKFLE HLYNSHIIFK AIISLNTINS HKFRLLQSPD PGTIIILLPN
     SDGLKGAPLR YFVVSILQKQ DNDSIEANKL LGIYHSHTES KKYKIMLSKV ISLDITRLKL
     LSNSFVKYSL LISYYSQFKK SLKFDTHMLS WMLSQVTTIA SLSITDVYKN FIMAIYSDYS
     NIDDLINDKL ECRPRTLGHV FVMKHLFQGI TSAVEQLGKI NKNKLIADVN DEGELISTGF
     DPNLRLKLPI SQLSTNNPKE IIHESFILFY LGNKGLHGSP QELLNLYYTP MQFESEYTKM
     MTDCGLYCQE LGNNGNLSFS FQAMFLTSKV AYAKLLNNTD EIRRSLVKEM KMDEPIMSIK
     QFSSTKSMVS NSVPDMSIKD CNLNKNIDVI QLERYIDTSL ISDPMKYITL MNNSIESINQ
     ERLLIYKDKL NKGIVLLPKL ILTTFKGKSF IGLENHYYTK LVSGDYIKQT NTKVFDEFYR
     LTDEIQEEKL RGFYKGYITE GDLLVRIFNK DQRTTDDREI YTGNAQVRLC LYPLEMTFKS
     ICKKIPEEAI TISGDQKQRK LLEQRLALIK TKRQFNKSGY KTEIYSVSSD ASKWSARDLL
     PKFIISIATN PYLTSDEKYF LVYLLVRYYD KKIVLTDSAF SNALRFSRED INGKYEEMTN
     NFTQNWFNVR SNWLQGNLNM TSSFVHHCST IMTDTLLSIS AKHNGFEAVM TSMVHSDDST
     YDFLIAKNSK TSSYINNEAN MGRFIISLIT YSNKKHCITL NEKKTYISTF YKEFLSTTIV
     SNELFFFYMA DLMPISSDTS YKSPLEDLAS YTGYINNSFS HACPIQILKC AITLLNHLTL
     STYNMQYTSE KNPRCNIPNS TDLPIQIYPR YKLPLSLAGC IPYYSSDAYN ILDDIIKTLE
     KNKVIKNSLL EDVIDDETLD EYITLVNKQK PEYAKYIQAC LLTMDYTQYE RDDEDPYNIV
     DYDLSQKSII NVASINKGSR IKKTYTYKKY LENETDIRLT SCVNPMWCIS KPKDEVLIKN
     PILANYMNPN FKDSLIFSKS ALDYGRRIIG SNKSMDTLSS HAFEKEKKQG IKTIYKKLDD
     KISTVEISKQ SLQRFLECIY SVIKKSLVAL QVYYSKVQVL VKTRPEFTKV IMPRSVYAEE
     YGKNSNTSMV ENLLVEQYCE IEQVDSKVEK FISFCKHVLQ RCGDIKIYRD PEDIDDDFRK
     YIEFKYTLKD ATMGLIQPHQ HLAEYAFDVY NNKLIFQGLM VRYYIDICET ISNPSYNIPS
     YTSPNSIIMT LDSLMKRDEI SSKIYISHIR TNRFDEYWLS RFGMYVYENY FVKYKLGYRI
     KIAANEKLMP TMKKVRNLRE PFKFICSLVA NDPSLFIQMT ESPDFQISGW KYSDIIAEMK
     STTDFSYNLF LYMMNEINFQ TLMRVMNLNR RVWNHWLMKT DSEPSDPNAS IALYMYQSTV
     VKVQTKTIGG GVTFSMLLLR HGMQHRQAFD EISKKIASDY APQLRIANIT PQTSFGRLQF
     CVNEYGRTVK PGSYRSSCIC NVNIAALTDL KPDIAYKENT INQIVTIISP TFEGEFVFKL
     NTYCDSEYYT CVMLENLDLN RVMILDHLCR GKYLIENPEY FTEISDQITP GACLALFSNN
     VNNKLWSNTI DTSKFAKLVH IGNYLKTEHE ASIVTKLCDS LVAICALNGI DHTLSLKPDN
     FIKSLRQYKL SYGFHEEFYN NYKKNEREPY TELIMAIAST AGDPFQKVIL AIITIFKAYT
     DLFISYKTDE VEF