M1AP_MOUSE
ID M1AP_MOUSE Reviewed; 529 AA.
AC Q9Z0E1; Q3V2A0;
DT 02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 02-SEP-2008, sequence version 2.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Meiosis 1 arrest protein;
DE AltName: Full=Meiosis 1-arresting protein;
DE AltName: Full=Meiosis 1-associated protein;
DE AltName: Full=Spermatogenesis-associated protein 37;
GN Name=M1ap; Synonyms=D6Mm5e, Spata37;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=129/SvJ, and C57BL/6J;
RX PubMed=9927484;
RA Jang W., Hua A., Spilson S.V., Miller W., Roe B.A., Meisler M.H.;
RT "Comparative sequence of human and mouse BAC clones from the mnd2 region of
RT chromosome 2p13.";
RL Genome Res. 9:53-61(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryonic stem cell;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP SYNERGY DURING ACUTE MYELOID LEUKEMIA.
RX PubMed=15044690; DOI=10.1073/pnas.0400930101;
RA Castilla L.H., Perrat P., Martinez N.J., Landrette S.F., Keys R.,
RA Oikemus S., Flanegan J., Heilman S., Garrett L., Dutra A., Anderson S.,
RA Pihan G.A., Wolff L., Liu P.P.;
RT "Identification of genes that synergize with Cbfb-MYH11 in the pathogenesis
RT of acute myeloid leukemia.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:4924-4929(2004).
RN [5]
RP ALTERNATIVE SPLICING, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=16881047; DOI=10.1002/dvdy.20907;
RA Arango N.A., Huang T.T., Fujino A., Pieretti-Vanmarcke R., Donahoe P.K.;
RT "Expression analysis and evolutionary conservation of the mouse germ cell-
RT specific D6Mm5e gene.";
RL Dev. Dyn. 235:2613-2619(2006).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-516, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [7]
RP FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY,
RP AND DEVELOPMENTAL STAGE.
RX PubMed=23269666; DOI=10.1095/biolreprod.111.098673;
RA Arango N.A., Li L., Dabir D., Nicolau F., Pieretti-Vanmarcke R.,
RA Koehler C., McCarrey J.R., Lu N., Donahoe P.K.;
RT "Meiosis I arrest abnormalities lead to severe oligozoospermia in meiosis 1
RT arresting protein (M1ap)-deficient mice.";
RL Biol. Reprod. 88:76-76(2013).
CC -!- FUNCTION: Required for meiosis I progression during spermatogenesis.
CC {ECO:0000269|PubMed:23269666}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:23269666}.
CC -!- TISSUE SPECIFICITY: Expressed in germ cells of the testis. Expressed
CC from spermatogonia to spermatids. Expressed at very low levels in lung,
CC stomach, thymus. Not detected in Sertoli cells.
CC {ECO:0000269|PubMed:16881047, ECO:0000269|PubMed:23269666,
CC ECO:0000269|PubMed:9927484}.
CC -!- DEVELOPMENTAL STAGE: Germ cell-specific, but may also be expressed in
CC cells that require the presence of germ cells. Cranial to caudal
CC punctate expression is seen in the developing ovary starting at 13.5
CC dpc. At 13.5 dpc. expressed in the cranial part of the XX gonad. At
CC 14.5 dpc, expression extends to almost two-thirds of the XX gonad, and
CC by 15.5 dpc expression is seen throughout the entire organ. By 16.5
CC dpc, expression is only detected in the most caudal tip of the XX
CC gonad. Expression switches from the embryonic ovary to the postnatal
CC testis. {ECO:0000269|PubMed:16881047, ECO:0000269|PubMed:23269666}.
CC -!- DISRUPTION PHENOTYPE: Males show reduced testicular size and a tubular
CC defects, which led to severe oligozoospermia and infertility.
CC Spermatocytes are eliminated via apoptosis either at the pachytene
CC checkpoint, or later at the spindle checkpoint during metaphase I.
CC {ECO:0000269|PubMed:23269666}.
CC -!- MISCELLANEOUS: Seems to synergize with Cbfb and Myh11 during the onset
CC of acute myeloid leukemia.
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DR EMBL; AF084363; AAC95337.1; -; Genomic_DNA.
DR EMBL; AF084364; AAC95350.1; -; mRNA.
DR EMBL; AK131954; BAE20898.1; -; mRNA.
DR EMBL; BC119803; AAI19804.1; -; mRNA.
DR EMBL; BC122521; AAI22522.1; -; mRNA.
DR CCDS; CCDS39525.1; -.
DR RefSeq; NP_149070.2; NM_033079.2.
DR AlphaFoldDB; Q9Z0E1; -.
DR STRING; 10090.ENSMUSP00000109613; -.
DR iPTMnet; Q9Z0E1; -.
DR PhosphoSitePlus; Q9Z0E1; -.
DR PaxDb; Q9Z0E1; -.
DR PRIDE; Q9Z0E1; -.
DR ProteomicsDB; 292066; -.
DR Antibodypedia; 49889; 38 antibodies from 13 providers.
DR Ensembl; ENSMUST00000113980; ENSMUSP00000109613; ENSMUSG00000030041.
DR GeneID; 110958; -.
DR KEGG; mmu:110958; -.
DR UCSC; uc009clp.2; mouse.
DR CTD; 130951; -.
DR MGI; MGI:1315200; M1ap.
DR VEuPathDB; HostDB:ENSMUSG00000030041; -.
DR eggNOG; ENOG502QT91; Eukaryota.
DR GeneTree; ENSGT00390000005656; -.
DR HOGENOM; CLU_038694_0_0_1; -.
DR InParanoid; Q9Z0E1; -.
DR OMA; STYNPLQ; -.
DR OrthoDB; 1323138at2759; -.
DR PhylomeDB; Q9Z0E1; -.
DR TreeFam; TF329502; -.
DR BioGRID-ORCS; 110958; 1 hit in 73 CRISPR screens.
DR ChiTaRS; M1ap; mouse.
DR PRO; PR:Q9Z0E1; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q9Z0E1; protein.
DR Bgee; ENSMUSG00000030041; Expressed in seminiferous tubule of testis and 39 other tissues.
DR ExpressionAtlas; Q9Z0E1; baseline and differential.
DR Genevisible; Q9Z0E1; MM.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0031497; P:chromatin assembly; NAS:UniProtKB.
DR GO; GO:0007292; P:female gamete generation; IEP:UniProtKB.
DR GO; GO:0051308; P:male meiosis chromosome separation; IMP:UniProtKB.
DR GO; GO:0007127; P:meiosis I; IEA:InterPro.
DR GO; GO:0006396; P:RNA processing; NAS:UniProtKB.
DR GO; GO:0007283; P:spermatogenesis; IMP:UniProtKB.
DR InterPro; IPR033587; M1AP.
DR PANTHER; PTHR28642; PTHR28642; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Differentiation; Phosphoprotein; Reference proteome;
KW Spermatogenesis.
FT CHAIN 1..529
FT /note="Meiosis 1 arrest protein"
FT /id="PRO_0000349269"
FT REGION 180..201
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 504..529
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 181..198
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 516
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CONFLICT 373
FT /note="H -> P (in Ref. 1; AAC95337/AAC95350)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 529 AA; 58618 MW; 0B7A5F3ED9489253 CRC64;
MNRRKTTSRG TSAAMKISHQ PPRLLIVNIA VPSWVDICPN LCEALQNFFS IACSLMGPSR
MSLFSLYTVQ NQHECVLPFV QVRGNFIRLQ ACISELRMLQ VEGCHRPPHA LLPLAIEDGL
QQFKQYSSHM ASSAAQPWTS LEITVLTSRP GKEVVKELEE GLKDINLLSV RRLQVAEVTK
GIQERSDSPS PTEEPSNDES SILEADIVLE TLDNDVVSME VFFKAWLHNS ETDQENIHLL
LTPQSLPPPS RAKDHPICLK CDLQERFLSP SLLPGTADGV SRIDDPKGDI STLYQMASLA
SASPYKLQVV KALKSSGICE SLTYGLPFIL RPTSCWQLDW DELETNQQHF HALCHCLLKR
DWLLLARGEP LIHKHNQSLP ACSFYVITPS HSLTLLVKLV ATRELMLPGF FPLLSEDPPE
DSLKIIESTL DSLDLGLTYN PLHVGSHLYS HLSSAHAKPQ GRLYTSCASR GLRKGGQLQT
NRVRAAVVPL PVAPAPRRAL KMTAASKASS AAFLPSDSEE GEEERPSHT
