ID   M22_HRSVA               Reviewed;          90 AA.
AC   P88812;
DT   16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   23-FEB-2022, entry version 71.
DE   RecName: Full=Protein M2-2;
GN   Name=M2-2;
OS   Human respiratory syncytial virus A (strain A2).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC   Monjiviricetes; Mononegavirales; Pneumoviridae; Orthopneumovirus.
OX   NCBI_TaxID=11259;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=4009789; DOI=10.1128/jvi.55.1.101-110.1985;
RA   Elango N., Satake M., Venkatesan S.;
RT   "mRNA sequence of three respiratory syncytial virus genes encoding two
RT   nonstructural proteins and a 22K structural protein.";
RL   J. Virol. 55:101-110(1985).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Cold-passage attenuated;
RX   PubMed=8918930; DOI=10.1006/viro.1996.0618;
RA   Firestone C.Y., Whitehead S.S., Collins P.L., Murphy B.R., Crowe J.E. Jr.;
RT   "Nucleotide sequence analysis of the respiratory syncytial virus subgroup A
RT   cold-passaged (cp) temperature sensitive (ts) cpts-248/404 live attenuated
RT   virus vaccine candidate.";
RL   Virology 225:419-422(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Cold-passage attenuated;
RX   PubMed=9035372; DOI=10.1007/bf00366988;
RA   Crowe J.E. Jr., Firestone C.Y., Whitehead S.S., Collins P.L., Murphy B.R.;
RT   "Acquisition of the ts phenotype by a chemically mutagenized cold-passaged
RT   human respiratory syncytial virus vaccine candidate results from the
RT   acquisition of a single mutation in the polymerase (L) gene.";
RL   Virus Genes 13:269-273(1996).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Cold-passage attenuated;
RX   PubMed=7747420; DOI=10.1006/viro.1995.1178;
RA   Connors M., Crowe J.E. Jr., Firestone C.Y., Murphy B.R., Collins P.L.;
RT   "A cold-passaged, attenuated strain of human respiratory syncytial virus
RT   contains mutations in the F and L genes.";
RL   Virology 208:478-484(1995).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Cold-passage attenuated;
RX   PubMed=9557743; DOI=10.1128/jvi.72.5.4467-4471.1998;
RA   Whitehead S.S., Juhasz K., Firestone C.Y., Collins P.L., Murphy B.R.;
RT   "Recombinant respiratory syncytial virus (RSV) bearing a set of mutations
RT   from cold-passaged RSV is attenuated in chimpanzees.";
RL   J. Virol. 72:4467-4471(1998).
RN   [6]
RP   FUNCTION.
RX   PubMed=10500164; DOI=10.1073/pnas.96.20.11259;
RA   Bermingham A., Collins P.L.;
RT   "The M2-2 protein of human respiratory syncytial virus is a regulatory
RT   factor involved in the balance between RNA replication and transcription.";
RL   Proc. Natl. Acad. Sci. U.S.A. 96:11259-11264(1999).
RN   [7]
RP   SUBCELLULAR LOCATION.
RX   PubMed=10466798; DOI=10.1099/0022-1317-80-8-2011;
RA   Ahmadian G., Chambers P., Easton A.J.;
RT   "Detection and characterization of proteins encoded by the second ORF of
RT   the M2 gene of pneumoviruses.";
RL   J. Gen. Virol. 80:2011-2016(1999).
RN   [8]
RP   FUNCTION.
RX   PubMed=10590093; DOI=10.1128/jvi.74.1.74-82.2000;
RA   Jin H., Cheng X., Zhou H.Z., Li S., Seddiqui A.;
RT   "Respiratory syncytial virus that lacks open reading frame 2 of the M2 gene
RT   (M2-2) has altered growth characteristics and is attenuated in rodents.";
RL   J. Virol. 74:74-82(2000).
RN   [9]
RP   FUNCTION.
RX   PubMed=16254330; DOI=10.1128/jvi.79.22.13943-13952.2005;
RA   Cheng X., Park H., Zhou H., Jin H.;
RT   "Overexpression of the M2-2 protein of respiratory syncytial virus inhibits
RT   viral replication.";
RL   J. Virol. 79:13943-13952(2005).
RN   [10]
RP   FUNCTION.
RX   PubMed=26474524; DOI=10.1016/j.virusres.2015.10.011;
RA   Asenjo A., Villanueva N.;
RT   "Phosphorylation of the human respiratory syncytial virus P protein
RT   mediates M2-2 regulation of viral RNA synthesis, a process that involves
RT   two P proteins.";
RL   Virus Res. 211:117-125(2016).
CC   -!- FUNCTION: Mediates the regulatory switch from transcription to RNA
CC       replication (PubMed:10500164) (Probable). Acts late in infection by
CC       inhibiting viral transcription and up-regulating RNA replication
CC       (PubMed:16254330, PubMed:26474524). Inhibition of transcription by
CC       protein M2-2 requires phosphorylation of the phosphoprotein
CC       (PubMed:26474524). {ECO:0000269|PubMed:10500164,
CC       ECO:0000269|PubMed:16254330, ECO:0000269|PubMed:26474524,
CC       ECO:0000305|PubMed:10590093}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000269|PubMed:10466798}.
CC   -!- SIMILARITY: Belongs to the orthopneumovirus M2-2 protein family.
CC       {ECO:0000305}.
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DR   EMBL; M11486; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; U50362; AAB86666.1; -; Genomic_RNA.
DR   EMBL; U50363; AAB86678.1; -; Genomic_RNA.
DR   EMBL; AF035006; AAC14904.1; -; Genomic_RNA.
DR   EMBL; U63644; AAC55972.1; -; Genomic_RNA.
DR   Proteomes; UP000007678; Genome.
DR   Proteomes; UP000134464; Genome.
DR   Proteomes; UP000181145; Genome.
DR   Proteomes; UP000181262; Genome.
DR   Proteomes; UP000181559; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   InterPro; IPR009969; Pneumo_M2-2.
DR   Pfam; PF07380; Pneumo_M2; 1.
PE   3: Inferred from homology;
KW   Host cytoplasm; Reference proteome; Viral RNA replication.
FT   CHAIN           1..90
FT                   /note="Protein M2-2"
FT                   /id="PRO_0000356857"
SQ   SEQUENCE   90 AA;  10674 MW;  9B7C5F1E4352ECF2 CRC64;
     MTMPKIMILP DKYPCSITSI LITSRCRVTM YNQKNTLYFN QNNPNNHMYS PNQTFNEIHW
     TSQELIDTIQ NFLQHLGIIE DIYTIYILVS