ID   M22_STRPY               Reviewed;         372 AA.
AC   P50469;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=M protein, serotype 2.2;
DE   Flags: Precursor;
GN   Name=emmL2.2;
OS   Streptococcus pyogenes.
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=1314;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=T2/44/RB4/119;
RX   PubMed=1370269; DOI=10.1128/iai.60.1.124-135.1992;
RA   Bessen D.E., Fischetti V.A.;
RT   "Nucleotide sequences of two adjacent M or M-like protein genes of group A
RT   streptococci: different RNA transcript levels and identification of a
RT   unique immunoglobulin A-binding protein.";
RL   Infect. Immun. 60:124-135(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Fischetti V.A., Bessen D.E.;
RT   "Immunoglobulin A binding protein.";
RL   Patent number US5556944, 17-SEP-1996.
CC   -!- FUNCTION: This protein is one of the different antigenic serotypes of
CC       protein M. Protein M is closely associated with virulence of the
CC       bacterium and can render the organism resistant to phagocytosis.
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000255|PROSITE-
CC       ProRule:PRU00477}; Peptidoglycan-anchor {ECO:0000255|PROSITE-
CC       ProRule:PRU00477}.
CC   -!- SIMILARITY: Belongs to the M protein family. {ECO:0000305}.
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DR   EMBL; X61276; CAA43582.1; -; Genomic_DNA.
DR   EMBL; I26204; -; NOT_ANNOTATED_CDS; Unassigned_DNA.
DR   PIR; S23326; S23326.
DR   AlphaFoldDB; P50469; -.
DR   SMR; P50469; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0006909; P:phagocytosis; IEA:UniProtKB-KW.
DR   InterPro; IPR019931; LPXTG_anchor.
DR   InterPro; IPR019950; M_anchor.
DR   InterPro; IPR003345; M_repeat.
DR   InterPro; IPR005877; YSIRK_signal_dom.
DR   Pfam; PF00746; Gram_pos_anchor; 1.
DR   Pfam; PF02370; M; 3.
DR   Pfam; PF04650; YSIRK_signal; 1.
DR   PRINTS; PR00015; GPOSANCHOR.
DR   TIGRFAMs; TIGR01168; YSIRK_signal; 1.
DR   PROSITE; PS50847; GRAM_POS_ANCHORING; 1.
PE   3: Inferred from homology;
KW   Cell wall; Coiled coil; Peptidoglycan-anchor; Phagocytosis; Repeat;
KW   Secreted; Signal; Virulence.
FT   SIGNAL          1..41
FT                   /evidence="ECO:0000255"
FT   CHAIN           42..342
FT                   /note="M protein, serotype 2.2"
FT                   /id="PRO_0000005613"
FT   PROPEP          343..372
FT                   /note="Removed by sortase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT                   /id="PRO_0000005614"
FT   REPEAT          131..153
FT                   /note="C-1"
FT   REPEAT          173..195
FT                   /note="C-2"
FT   REPEAT          222..244
FT                   /note="C-3"
FT   REGION          125..191
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          131..244
FT                   /note="3 X repeats, type C"
FT   REGION          211..274
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          295..344
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           339..343
FT                   /note="LPXTG sorting signal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT   COMPBIAS        125..169
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        211..243
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        260..274
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        318..344
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         342
FT                   /note="Pentaglycyl murein peptidoglycan amidated threonine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
SQ   SEQUENCE   372 AA;  41149 MW;  E8FD5D0920C95C74 CRC64;
     MARQQTKKNY SLRKLKTGTA SVAVALTVLG AGFANQTEVR ADEAKKMEVK ESEKESQYKT
     LALRGENADL RNVNAKYLEK INAEEEKNKK LEAINKELNE NYYKLQDGID ALEKEKEDLK
     TTLAKTTKEN EISEASRKGL SRDLEASRTA KKELEAKHQK LEAENKKLTE GNQVSEASRK
     GLSNDLEASR AAKKELEAKY QKLETDHQAL EAKHQKLEAD YQVSETSRKG LSRDLEASRE
     ANKKVTSELT QAKAQLSALE ESKKLSEKEK AELQAKLDAQ GKALKEQLAK QTEELAKLRA
     EKAAGSKTPA TKPANKERSG RAAQTATRPS QNKGMRSQLP STGEAANPFF TAAAATVMVS
     AGMLALKRKE EN